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EFFECTS OF DIFFERENT CONCENTRATIONS OF DEXTROSE AND YEAST EXTRACT ON THE DEVELOPMENT OF THE ENTOMOPATHOGENIC FUNGUS LECANICILLIUM LECANII ON LIQUID FERMENTATION

ABSTRACT

The strain JAB 02 of the entomopathogenic fungus Lecanicillium lecanii was cultivated in liquid media with different concentrations of dextrose (D) and yeast extract (YE) sources (60g D – 60 g YE, 60 g D – 40 g YE, 60 g D – 20 g YE, 60 g D – 10 g YE, 40 g D – 40 g YE, 40 g D – 20 g YE, 40 g D – 10 g YE, 20 g D – 60 g YE, 20 g D – 40g YE). The dextrose was used as carbon source and the yeast extract as a nitrogen source. The objective of this research was to evaluate the dry biomass and the sporulation of this strain. The fungus was cultivated for ten days after inoculation, with evaluations each 2 days. The combinations of dextrose and yeast extract tending to wand equals parts of each element were more efficient in regard to dry biomass. For sporulation, 8 days of growth are sufficient for greatest production of conidia. This strain is demands in carbon since the combinations 60 g D – 60 g YE, 60 g D – 40 g YE, 60 g D – 20 g YE, 60 g D – 10 g YE, 40 g D – 40 g YE did not differ statistically.

KEY WORDS
Lecanicillium lecanii ; liquid fermentation; biological control

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