Efeito da deficiência de potássio sobre as atividades de glutamato desidrogenase e glutamato sintase em feijoeiro (Phaseolus vulgaris L.)

Gutierrez, L.E.; Crocomo, O.J.; Rossi, C.

doi:10.1590/S0071-12761989000100009

Resumos

Foi estudado o efeito da deficiência de potássio sobre as atividades da glutamato desidrogenase (EC. 1.4.1.2) e glutamato sintase (EC. 2.6.1.53) em folhas de feijoeiro (Phaseolus vulgaris L.). Os resultados mostraram que a atividade específica da glutamato desidrogenase aumentou nas plantas deficientes em potássio nos dois cultivares estudados. Foi detectada redução na atividade de glutamato sintase nas plantas deficientes em potássio.

potássio; glutamato desidrogenase; glutamato sintase; Phaseolus vulgaris

The effect of potassium deficiency on glutamate dehydrogenase(EC. 1.4.1.2) and glutamate synthase (EC. 2.6.1.53) activities in leaves of two cultivars of bean plants (Phaseolus vulgaris L.) was studied. The results showed an increase of glutamate dehydrogenase and a decrease of glutamate synthase activities in potassium deficient plants of the two cultivars studied.

potassium; glutamate dehydrogenase; glutamate synthase; Phaseolus vulgaris

ARTIGOS

Efeito da deficiência de potássio sobre as atividades de glutamato desidrogenase e glutamato sintase em feijoeiro (Phaseolus vulgaris L.)

Effect of potassium deficiency on glutamate dehydrogenase and glutamate synthase activities in bean leaves (Phaseolus vulgaris L.)

L.E. Gutierrez^I; O.J. Crocomo^I; C. Rossi^II

^IDepartamento de Química da E.S.A."Luiz de Queiroz" da Universidade de São Paulo, 13.400 - Piracicaba, SP e CEBTEC/FEALQ

^IIDepartamento de Bioquímica do Instituto Básico de Biologia Médica e Agrícola da UNESP, 18.610 - Botucatu, SP

RESUMO

Foi estudado o efeito da deficiência de potássio sobre as atividades da glutamato desidrogenase (EC. 1.4.1.2) e glutamato sintase (EC. 2.6.1.53) em folhas de feijoeiro (Phaseolus vulgaris L.). Os resultados mostraram que a atividade específica da glutamato desidrogenase aumentou nas plantas deficientes em potássio nos dois cultivares estudados. Foi detectada redução na atividade de glutamato sintase nas plantas deficientes em potássio.

Termos para indexação: potássio, glutamato desidrogenase, glutamato sintase, Phaseolus vulgaris.

ABSTRACT

The effect of potassium deficiency on glutamate dehydrogenase(EC. 1.4.1.2) and glutamate synthase (EC. 2.6.1.53) activities in leaves of two cultivars of bean plants (Phaseolus vulgaris L.) was studied. The results showed an increase of glutamate dehydrogenase and a decrease of glutamate synthase activities in potassium deficient plants of the two cultivars studied.

Index terms: potassium, glutamate dehydrogenase, glutamate synthase, Phaseolus vulgaris.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Recebido para publicação em: 29/03/89

Aprovado para publicação em: 12/06/89

BARASH, I.; MOR, H.; SADON, T. Isoenzymes of glutamate dehydrogenase from oat leaves: properties and light effect on synthesis. Plant and Cell Physiology , Tokyo, 17:493-500, 1976.
BASSO, L.C. Significado bioquímico da deficiência de potássio na formação de aminas em gergelim (Sesamum indicum). Botucatu, 1973. 76p. (Doutorado - Faculdade de Ciências Médicas e Biológicas de Botucatu) .
BOULTER, D. Protein synthesis in plants. Annual Review of Plant Physiology, Stanford, 21:93-114, 1970.
BULEN, W.A. The isolation and characterization of glutamic dehydrogenase from corn leaves. Archives of Biochemistry and Biophysics, New York, 62:173 - 83, 1956.
CHOU, K. & SPLITTSTOESSER, W.E. Glutamate dehydrogenase from pumpkin cotyledons. Characterization and isoenzymes. Plant Physiology, Lancaster, 49: 550-4, 1972.
CR0C0M0, O.J. & BASSO, L.C. Accumulation of putrescine and related amino acids in potassium deficient Sesamum. Phytochemistry, Oxford, 13:2659-65, 1974.
CR0C0M0, O.J.; BASSO, L.C; BRASIL, O.G. Formation of N-carbamylputrescine from citrulline in Sesamum. Phytochemistry, Oxford, 9:537-40, 1970.
DIXON, M. & WEBB, E.C. Enzymes. 2.ed. New York, Academic Press, 1964. 950p.
DOUGALL, D.K. Evidence for the presence of glutamate synthase in extracts of carrot cell cultures. Biochemical and Biophysical Research Communication, New York, 58:639-46, 1974.
DOUGALL, D.K. & BLOCH, J. A survey of the presence of glutamate synthase in plant cell suspension cultures. Canadian Journal of Botany, Ottawa, 54:2924-7, 1976.
EATON, S.W. Effects of potassium deficiency on growth and metabolism of sunflower plants . Botanical Gazette, Chicago, 114:165-80, 1952.
FAHIEN, L.A. & COHEN, P.P. L-glutamate dehydrogenase. Methods in Enzymology, New York, 17A:839-44, 1970.
GUTIERREZ, L.E. Atividade de enzimas relacionadas com a assimilação do nitrogênio afetada pela deficiência de potássio e maturidade em feijoeiro (Phaseolus vulgaris L.). São Paulo, 1977. 108p. (Doutorado -Instituto de Química/USP).
GUTIERREZ, L.E.; CROCOMO, O.J.; BASSO, L.C. Efeito da deficiência de potássio sobre alguns aspectos metabólicos em feijoeiro (Phaseolus vulgaris L.) Revista de Agricultura, Piracicaba, 53(4):209-16, 1978.
HARPER, J.E. & PAULSEN, G.M. Nitrogen assimilation and protein synthesis in wheat seedlings as affected by mineral nutrition.I. Macronutrients. Plant Physiology, Lancaster, 44:69-74, 1969.
KANAMORI, T.; KONISHI, S.; TAKAHASHI, E. Inducible, formation of glutamate dehydrogenase in rice plant roots by the assition of ammonia to the media. Physiologia Plantarum, Kobenhavn, 26:1-6, 1972.
KING, J. & WU, W.Y. Partial purification and kinetic properties of glutamic dehydrogenase from soybean cotyledons. Phytochemistry, Oxford,10:915-28, 1971.
LOWRY, O.H.; ROSEBROUGH, N.J.; FARR, A.L.; RANDALL, R.J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, Baltimore, 193: 265-75, 1951.
MACLEOD, A.B. & SUZUKI, M. Effect of potassium on the content of amino acids₊ in alfafa and orchardgrass grown with NO@ and NH@ nitrogen in nutrient solution cultures. Crop Science, Madison, 7:599-605, 1967.
MIFLIN, B.J. & LEA, P.J. The pathway of nitrogen assimilation in plants. Phytochemistry, Oxford, 15: 873-85, 1976.
PALICH, E. & JOY, K.W. Glutamate dehydrogenase from pea roots: purification and properties of the enzyme. Canadian Journal of Botany, Ottawa, 49:127-38, 1971.
PATNAIK, R.; BARKER, A.V.; MAYNARD, D.N. Effect of ammonium and potassium ions on some physiological and biochemical process of excised cucumber cotyledons. Physiologia Plantarum, Kobenhavn, 27:32-6, 1972.
PIMENTEL GOMES, F. Curso de estatística experimental. São Paulo, Nobel, 1970. 430p.
RHODES, D.; RENDON, G.A.; STEWART, G.R. The regulation of ammonia assimilating enzymes in Lemma minor. Planta, Berlin, 129:203-10, 1976.
ROSSI, C; CROCOMO, O.J.; GUTIERREZ, L.E. Efeito de fontes de nitrogênio na atividade de desidrogenase glutâmica em feijoeiro (Phaseolus vulgaris L.). Acta Biologica Leopoldensia, São Leopoldo, 4(2):105-14, 1982.
ROSSI, C; GUTIERREZ, L.E.; CROCOMO, O.J. Sintase de glutamato (GOGAT) em plântulas de feijoeiro (Phaseolus vulgaris L.). In: REUNIÃO DA SOCIEDADE BRASILEIRA PARA O PROGRESSO DA CIÊNCIA, 32., Rio de Janeiro, 1980. p.855. (Resumos).
SMITH, T.A. Amine levels in mineral deficient Hordewt vulgare leaves. Phytochemistry, Oxford, 12:2093-100, 1973.
WAKIUCHI, N.; MATSUMOTO, H.; TAKAHASHI, E. Changes of some enzymes activities of Cucumber during ammonium toxicity. Physiologia Plantarum, Kobenhavn, 24:248-51, 1971.
WEISSMAN, G.S. Influence of ammonium and nitrate nutrition on enzymatic activity in soybean and sunflower. Plant Physiology, Lancaster, 49:138-41, 1972.
YAMASAKI, K. & SUZUKI, Y. Some properties of glutamate dehydrogenase from pea seedlings. Phytochemistry, Oxford, 8:963-9, 1969.

Datas de Publicação

Publicação nesta coleção
06 Maio 2009
Data do Fascículo
1989

Histórico

Recebido
29 Mar 1989
Aceito
12 Jun 1989

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] BARASH, I.; MOR, H.; SADON, T. Isoenzymes of glutamate dehydrogenase from oat leaves: properties and light effect on synthesis. Plant and Cell Physiology , Tokyo, 17:493-500, 1976.

[2] BASSO, L.C. Significado bioquímico da deficiência de potássio na formação de aminas em gergelim (Sesamum indicum). Botucatu, 1973. 76p. (Doutorado - Faculdade de Ciências Médicas e Biológicas de Botucatu) .

[3] BOULTER, D. Protein synthesis in plants. Annual Review of Plant Physiology, Stanford, 21:93-114, 1970.

[4] BULEN, W.A. The isolation and characterization of glutamic dehydrogenase from corn leaves. Archives of Biochemistry and Biophysics, New York, 62:173 - 83, 1956.

[5] CHOU, K. & SPLITTSTOESSER, W.E. Glutamate dehydrogenase from pumpkin cotyledons. Characterization and isoenzymes. Plant Physiology, Lancaster, 49: 550-4, 1972.

[6] CR0C0M0, O.J. & BASSO, L.C. Accumulation of putrescine and related amino acids in potassium deficient Sesamum. Phytochemistry, Oxford, 13:2659-65, 1974.

[7] CR0C0M0, O.J.; BASSO, L.C; BRASIL, O.G. Formation of N-carbamylputrescine from citrulline in Sesamum. Phytochemistry, Oxford, 9:537-40, 1970.

[8] DIXON, M. & WEBB, E.C. Enzymes. 2.ed. New York, Academic Press, 1964. 950p.

[9] DOUGALL, D.K. Evidence for the presence of glutamate synthase in extracts of carrot cell cultures. Biochemical and Biophysical Research Communication, New York, 58:639-46, 1974.

[10] DOUGALL, D.K. & BLOCH, J. A survey of the presence of glutamate synthase in plant cell suspension cultures. Canadian Journal of Botany, Ottawa, 54:2924-7, 1976.

[11] EATON, S.W. Effects of potassium deficiency on growth and metabolism of sunflower plants . Botanical Gazette, Chicago, 114:165-80, 1952.

[12] FAHIEN, L.A. & COHEN, P.P. L-glutamate dehydrogenase. Methods in Enzymology, New York, 17A:839-44, 1970.

[13] GUTIERREZ, L.E. Atividade de enzimas relacionadas com a assimilação do nitrogênio afetada pela deficiência de potássio e maturidade em feijoeiro (Phaseolus vulgaris L.). São Paulo, 1977. 108p. (Doutorado -Instituto de Química/USP).

[14] GUTIERREZ, L.E.; CROCOMO, O.J.; BASSO, L.C. Efeito da deficiência de potássio sobre alguns aspectos metabólicos em feijoeiro (Phaseolus vulgaris L.) Revista de Agricultura, Piracicaba, 53(4):209-16, 1978.

[15] HARPER, J.E. & PAULSEN, G.M. Nitrogen assimilation and protein synthesis in wheat seedlings as affected by mineral nutrition.I. Macronutrients. Plant Physiology, Lancaster, 44:69-74, 1969.

[16] KANAMORI, T.; KONISHI, S.; TAKAHASHI, E. Inducible, formation of glutamate dehydrogenase in rice plant roots by the assition of ammonia to the media. Physiologia Plantarum, Kobenhavn, 26:1-6, 1972.

[17] KING, J. & WU, W.Y. Partial purification and kinetic properties of glutamic dehydrogenase from soybean cotyledons. Phytochemistry, Oxford,10:915-28, 1971.

[18] LOWRY, O.H.; ROSEBROUGH, N.J.; FARR, A.L.; RANDALL, R.J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, Baltimore, 193: 265-75, 1951.

[19] MACLEOD, A.B. & SUZUKI, M. Effect of potassium on the content of amino acids₊ in alfafa and orchardgrass grown with NO@ and NH@ nitrogen in nutrient solution cultures. Crop Science, Madison, 7:599-605, 1967.

[20] MIFLIN, B.J. & LEA, P.J. The pathway of nitrogen assimilation in plants. Phytochemistry, Oxford, 15: 873-85, 1976.

[21] PALICH, E. & JOY, K.W. Glutamate dehydrogenase from pea roots: purification and properties of the enzyme. Canadian Journal of Botany, Ottawa, 49:127-38, 1971.

[22] PATNAIK, R.; BARKER, A.V.; MAYNARD, D.N. Effect of ammonium and potassium ions on some physiological and biochemical process of excised cucumber cotyledons. Physiologia Plantarum, Kobenhavn, 27:32-6, 1972.

[23] PIMENTEL GOMES, F. Curso de estatística experimental. São Paulo, Nobel, 1970. 430p.

[24] RHODES, D.; RENDON, G.A.; STEWART, G.R. The regulation of ammonia assimilating enzymes in Lemma minor. Planta, Berlin, 129:203-10, 1976.

[25] ROSSI, C; CROCOMO, O.J.; GUTIERREZ, L.E. Efeito de fontes de nitrogênio na atividade de desidrogenase glutâmica em feijoeiro (Phaseolus vulgaris L.). Acta Biologica Leopoldensia, São Leopoldo, 4(2):105-14, 1982.

[26] ROSSI, C; GUTIERREZ, L.E.; CROCOMO, O.J. Sintase de glutamato (GOGAT) em plântulas de feijoeiro (Phaseolus vulgaris L.). In: REUNIÃO DA SOCIEDADE BRASILEIRA PARA O PROGRESSO DA CIÊNCIA, 32., Rio de Janeiro, 1980. p.855. (Resumos).

[27] SMITH, T.A. Amine levels in mineral deficient Hordewt vulgare leaves. Phytochemistry, Oxford, 12:2093-100, 1973.

[28] WAKIUCHI, N.; MATSUMOTO, H.; TAKAHASHI, E. Changes of some enzymes activities of Cucumber during ammonium toxicity. Physiologia Plantarum, Kobenhavn, 24:248-51, 1971.

[29] WEISSMAN, G.S. Influence of ammonium and nitrate nutrition on enzymatic activity in soybean and sunflower. Plant Physiology, Lancaster, 49:138-41, 1972.

[30] YAMASAKI, K. & SUZUKI, Y. Some properties of glutamate dehydrogenase from pea seedlings. Phytochemistry, Oxford, 8:963-9, 1969.

Brasil

Brasil

Efeito da deficiência de potássio sobre as atividades de glutamato desidrogenase e glutamato sintase em feijoeiro (Phaseolus vulgaris L.)

Effect of potassium deficiency on glutamate dehydrogenase and glutamate synthase activities in bean leaves (Phaseolus vulgaris L.)

Resumos

Datas de Publicação

Histórico