Propriedades da transcarbamilase de ornitina parcialmente purificada de folhas de feijoeiro (Phaseolus vulgaris L.)

Gutierrez, L.E.; Crocomo, O.J.

doi:10.1590/S0071-12761989000100008

Resumos

Transcarbamilase de ornitina (E.C. 2.1.3.3) extraída de folhas de feijoeiro (Phaseolus vulgaris L. cv. carioca) com 31 dias de maturidade foi parcialmente purificada com sulfato de amônio em pH 7,2 e 7,8. A enzima apresentou as seguintes propriedades: pH ótimo 7,8, temperatura ótima 40°C, Constante de Michaelis para ornitina de 3,18mM e para carbamil-fosfato de 3,93mM.

transcarbamilase de ornitina; feijoeiro; Phaseolus vulgaris

Ornithine transcarbamylase (E.C. 2.1.3.3) was extracted from leaves of bean plants (Phaseolus vulgaris L. cv. carioca) and partially purified by ammonium sulfate precipitation at pH 7,2 and 7.8. The enzyme preparation showed an optimum pH of 7.8 and Km of 3.18mM for ornithine and of 3.93mM for carbamil-phosphate.

ornithine transcarbamylase; bean; Phaseolus vulgaris

ARTIGOS

Propriedades da transcarbamilase de ornitina parcialmente purificada de folhas de feijoeiro (Phaseolus vulgaris L.)

Partial purification and properties of ornithine transcarbamylase from bean leaves (Phaseolus vulgaris L.)

L.E. Gutierrez; O.J. Crocomo

Departamento de Química da E.S.A."Luiz de Queiroz" da Universidade de São Paulo, 13.400 - Piracicaba, SP. e CEBTEC/FEALQ

RESUMO

Transcarbamilase de ornitina (E.C. 2.1.3.3) extraída de folhas de feijoeiro (Phaseolus vulgaris L. cv. carioca) com 31 dias de maturidade foi parcialmente purificada com sulfato de amônio em pH 7,2 e 7,8. A enzima apresentou as seguintes propriedades: pH ótimo 7,8, temperatura ótima 40°C, Constante de Michaelis para ornitina de 3,18mM e para carbamil-fosfato de 3,93mM.

Termos para indexação: transcarbamilase de ornitina, feijoeiro, Phaseolus vulgaris.

ABSTRACT

Ornithine transcarbamylase (E.C. 2.1.3.3) was extracted from leaves of bean plants (Phaseolus vulgaris L. cv. carioca) and partially purified by ammonium sulfate precipitation at pH 7,2 and 7.8. The enzyme preparation showed an optimum pH of 7.8 and Km of 3.18mM for ornithine and of 3.93mM for carbamil-phosphate.

Index terms: ornithine transcarbamylase, bean,Phaseolus vulgaris.

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Recebido para publicação em: 29/03/89

Aprovado para publicação em: 12/06/89

ARCHIBALD, E.M. Determination of citruline and allantoin and demonstration of citrulline in blood plasma. Journal of Biological Chemistry, Baltimore, 156: 121-42, 1944.
BOGGESS, S.F. & NAYLOR, A.W. Partial purification and properties of ornithine transcarbamylase from Nostoc muscorum. Plant Physiology, Lancaster, 56:640-4, 1975.
BONE, D.H. Metabolism of citrulline and ornithine in mung bean mitochondria. Plant Physiology, Lancaster, 34:171-5, 1959.
BROWN, G.W. & COHEN, P.P. Comparative biochemistry of urea synthesis. I. Methods for the quantitative assay of urea cycle enzymes in liver. Journal of Biological Chemistry, Baltimore, 234:1769-74, 1959.
CARAVACA, J. & GRISOLIA, S. Synthesis of citrulline with animal and bacterial enzymes. Journal of Biological Chemistry, Baltimore, 235:684-93, 1960.
DIXON, M. & WEBB, E.C. Enzymes. 2 .ed. New York, Academic Press, 1964. 950p.
EID, S.; WALY, Y.; ABDELAL, A.T. Separation and properties of two ornithine carbamoyl - transferases from Pisum sativum seedlings . Phytochemistry, Oxford, 13:99-102, 1974.
GUTIERREZ, L.E. Atividade de enzimas relacionadas com a assimilação do nitrogênio afetada pela deficiência de potássio e maturidade em feijoeiro (Phaseolus vulgaris L.). São Paulo, 1977. 108p. (Doutorado -Instituto de Química/USP).
JOSEPH, R.L.; BALDWIN, E.; WATTS, D.C. Studies on carbamoyl phosphate-L-ornithine carbamoyl-transferase from of liver. Biochemical Journal, London, 87:409-16, 1963.
KASTING, R. & DELWICHE, C. Ornithine, citrulline and arginine metabolism in water melon seedlings. Plant Physiology, Lancaster, 33:350-4, 1958.
KLECZKOWSKI, K. Biosynthesis of citrulline in bean seedlings. Bulletin ale l'Academie Polonaise des Sciences. Serie des Sciences Chimiques, Varsovie, 5: 83-7, 1957. Apud Chemical Abstracts, Columbus, 54: 1662c, 1960.
KLECZKOWSKI, K. & COHEN, P.P. Purification of ornithine transcarbamylase from pea seedlings. Archives of Biochemistry and Biophysics, New York, 107:271-8, 1964.
KOLLOFFEL, C. & STROABAND, H.W.J. Ornithine carbamoyltransferase activity from the cotyledons of developing and germinating seeds of Vicia faba. Photochemistry, Oxford, 12:2635-8, 1973.
LOWRY, O.H.; ROSEBROUGH,N.J.; FARR, A.L.; RANDALL, R.J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, Baltimore, 193: 265-75, 1951.
RAMOS, F.; STALON, V.; PIERARD, A.; WIAME, J.M. The specialization of the two ornithine carbamoyl-transferases of Pseudomonas. Biochimica et Biophysica Acta, Amsterdam, 139:98-106, 1967.
SPECTOR, L. & JONES, M.E. Acetyl - glutamic acid. Methods in Enzymology, New York, 6:557-62, 1963.
SPENCER, P.W. & TITUS, J.S. The occurrence and nature of ornithine carbamoyl-transferase in senescing apple leaf tissue. Plant Physiology, Lancaster,54: 382-5, 1974.
SPLITTSTOESSER, W.E. Metabolism of arginine by aging in 7 days old punpkin seedlings. Plant Physiology, Lancaster, 44:361-6, 1969.
STALON, V.; RAMOS, P.; PERARD, A.; WIAME, J.M. The occurrence of a carbamoyltransferase in Pseudomonas. Biochimica et Biophysica Acta, Amsterdam, 139:91-7, 1967.
TAM, L.Q. & PATIL, S.S. Mode of action of the toxin from Pseudomonas phaseolicola. II. Mechanism of inhibition of bean ornithine carbamoyltransferase. Plant Physiology, Lancaster, 49:808-12, 1972.
WEISS, R.L. & DAVIS, R.H. Intra-celular localization of enzymes of arginine metabolism in Neurospora. Journal of Biological Chemistry, Baltimore, 248: 5403-8, 1973.

Datas de Publicação

Publicação nesta coleção
06 Maio 2009
Data do Fascículo
1989

Histórico

Aceito
12 Jun 1989
Recebido
29 Mar 1989

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] ARCHIBALD, E.M. Determination of citruline and allantoin and demonstration of citrulline in blood plasma. Journal of Biological Chemistry, Baltimore, 156: 121-42, 1944.

[2] BOGGESS, S.F. & NAYLOR, A.W. Partial purification and properties of ornithine transcarbamylase from Nostoc muscorum. Plant Physiology, Lancaster, 56:640-4, 1975.

[3] BONE, D.H. Metabolism of citrulline and ornithine in mung bean mitochondria. Plant Physiology, Lancaster, 34:171-5, 1959.

[4] BROWN, G.W. & COHEN, P.P. Comparative biochemistry of urea synthesis. I. Methods for the quantitative assay of urea cycle enzymes in liver. Journal of Biological Chemistry, Baltimore, 234:1769-74, 1959.

[5] CARAVACA, J. & GRISOLIA, S. Synthesis of citrulline with animal and bacterial enzymes. Journal of Biological Chemistry, Baltimore, 235:684-93, 1960.

[6] DIXON, M. & WEBB, E.C. Enzymes. 2 .ed. New York, Academic Press, 1964. 950p.

[7] EID, S.; WALY, Y.; ABDELAL, A.T. Separation and properties of two ornithine carbamoyl - transferases from Pisum sativum seedlings . Phytochemistry, Oxford, 13:99-102, 1974.

[8] GUTIERREZ, L.E. Atividade de enzimas relacionadas com a assimilação do nitrogênio afetada pela deficiência de potássio e maturidade em feijoeiro (Phaseolus vulgaris L.). São Paulo, 1977. 108p. (Doutorado -Instituto de Química/USP).

[9] JOSEPH, R.L.; BALDWIN, E.; WATTS, D.C. Studies on carbamoyl phosphate-L-ornithine carbamoyl-transferase from of liver. Biochemical Journal, London, 87:409-16, 1963.

[10] KASTING, R. & DELWICHE, C. Ornithine, citrulline and arginine metabolism in water melon seedlings. Plant Physiology, Lancaster, 33:350-4, 1958.

[11] KLECZKOWSKI, K. Biosynthesis of citrulline in bean seedlings. Bulletin ale l'Academie Polonaise des Sciences. Serie des Sciences Chimiques, Varsovie, 5: 83-7, 1957. Apud Chemical Abstracts, Columbus, 54: 1662c, 1960.

[12] KLECZKOWSKI, K. & COHEN, P.P. Purification of ornithine transcarbamylase from pea seedlings. Archives of Biochemistry and Biophysics, New York, 107:271-8, 1964.

[13] KOLLOFFEL, C. & STROABAND, H.W.J. Ornithine carbamoyltransferase activity from the cotyledons of developing and germinating seeds of Vicia faba. Photochemistry, Oxford, 12:2635-8, 1973.

[14] LOWRY, O.H.; ROSEBROUGH,N.J.; FARR, A.L.; RANDALL, R.J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, Baltimore, 193: 265-75, 1951.

[15] RAMOS, F.; STALON, V.; PIERARD, A.; WIAME, J.M. The specialization of the two ornithine carbamoyl-transferases of Pseudomonas. Biochimica et Biophysica Acta, Amsterdam, 139:98-106, 1967.

[16] SPECTOR, L. & JONES, M.E. Acetyl - glutamic acid. Methods in Enzymology, New York, 6:557-62, 1963.

[17] SPENCER, P.W. & TITUS, J.S. The occurrence and nature of ornithine carbamoyl-transferase in senescing apple leaf tissue. Plant Physiology, Lancaster,54: 382-5, 1974.

[18] SPLITTSTOESSER, W.E. Metabolism of arginine by aging in 7 days old punpkin seedlings. Plant Physiology, Lancaster, 44:361-6, 1969.

[19] STALON, V.; RAMOS, P.; PERARD, A.; WIAME, J.M. The occurrence of a carbamoyltransferase in Pseudomonas. Biochimica et Biophysica Acta, Amsterdam, 139:91-7, 1967.

[20] TAM, L.Q. & PATIL, S.S. Mode of action of the toxin from Pseudomonas phaseolicola. II. Mechanism of inhibition of bean ornithine carbamoyltransferase. Plant Physiology, Lancaster, 49:808-12, 1972.

[21] WEISS, R.L. & DAVIS, R.H. Intra-celular localization of enzymes of arginine metabolism in Neurospora. Journal of Biological Chemistry, Baltimore, 248: 5403-8, 1973.

Brasil

Brasil

Propriedades da transcarbamilase de ornitina parcialmente purificada de folhas de feijoeiro (Phaseolus vulgaris L.)

Partial purification and properties of ornithine transcarbamylase from bean leaves (Phaseolus vulgaris L.)

Resumos

Datas de Publicação

Histórico