Resumo
During the oxidation of the substrate, both Nitrosomonas and Nitrobacter have part of the energy made available as high energy phosphate, mamely ADP and ATP. This chemical energy is used to fix CO2. The nature of the reducing power is unknown at present. Active cells of Nitrobacter were shown to fix CO2 along the same pathway as found in higher plant photosynthesis. Sonic extracts of Nitrosomonas and Nitrobacter when incubated with NaH14CO3 and cofactors showed two ports of entry of CO2 into organic compounds one being, as expected, the carboxidismutase reaction. On protein basis an equivalent amount of CO2 was, however, incorporated via the oxaloacetic carboxylase reation. It is clear then that both micoorganisms possess typical autotrophic and heterotrophic mechanisms for the fixation of CO2 which is required for the primary synthesis of cell material.
ICentro Nacional de Energia Nuclear na Agricultura
IIEscola Superior de Agricultura "Luiz de Queiroz" Universidade de São Paulo. Piracicaba, São Paulo - Brasil
IIIKearney Foundation of Soil Science University of California Davis, California, Est. Unid. Amer. Norte
ABSTRACT
During the oxidation of the substrate, both Nitrosomonas and Nitrobacter have part of the energy made available as high energy phosphate, mamely ADP and ATP. This chemical energy is used to fix CO2. The nature of the reducing power is unknown at present.
Active cells of Nitrobacter were shown to fix CO2 along the same pathway as found in higher plant photosynthesis.
Sonic extracts of Nitrosomonas and Nitrobacter when incubated with NaH14CO3 and cofactors showed two ports of entry of CO2 into organic compounds one being, as expected, the carboxidismutase reaction. On protein basis an equivalent amount of CO2 was, however, incorporated via the oxaloacetic carboxylase reation. It is clear then that both micoorganisms possess typical autotrophic and heterotrophic mechanisms for the fixation of CO2 which is required for the primary synthesis of cell material.
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Fixação do dióxido de carbono por bactérias nitrificantes
Datas de Publicação
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Publicação nesta coleção
27 Jun 2012 -
Data do Fascículo
1964
