Menezes, Humberto

doi:10.1590/S0037-86821971000400006

Resumos

Drogas imunossupressoras (Azathioprina e Methotrexato) que se mostraram capazes de agravar infecções experimentais por Trypanosoma cruzi virulento (cepa Y), em camundongos, não conseguiram favorecer o aparecimento de qualquer sinal de infecção em animais vacinados com a cepa avirulenta PF do mesmo tripanosoma. Vinblastine, quando dado 3 dias antes da vacina, não favoreceu parasitemias positivas nem mortalidade significativamente aumentada, mas induziu o aparecimento de hemoculturas positivas. Os tripanosomas dessas culturas foram incapazes de infectar camundongos jovens. Quando o Vinblastine foi usado 15 dias após a vacinação dos animais, todos os testes foram negativos, inclusive as hemoculturas

Antiproliferative drugs (Azathioprine and Methotrexate) were able to enhance a T. cruzi virulent infection in mice, but failed to induce evident infection in animais previously vaccinated with the live PF strain of the same parasite. Vinblastine (Vbl) had a similar effect with the virulent Y strain, but promoted the appearance of positive blood eultures in animais vaecinated and treated previously with the drug. Negative results are obtained if the immunosupressive agent is used 15 days after the vaccine. The flagellates isolated from the vaccinated Vbl treated mice remained avirulent for baby mice, after i. p. inoculation. The immunity induced by the PF strain is probably sterile i. e. does not imply in the persistence of a latent infection (premunition).

The avirulence of the cultivated Y strain of Trypanosoma cruzi. IV. The effect of immunosuppressive agents in mice

Humberto Menezes

U.S.P. Departamento de Patologia - Faculdade de Medicina - Ribeirão Prêto, São Paulo. Brasil

ABSTRACT

Antiproliferative drugs (Azathioprine and Methotrexate) were able to enhance a T. cruzi virulent infection in mice, but failed to induce evident infection in animais previously vaccinated with the live PF strain of the same parasite.

Vinblastine (Vbl) had a similar effect with the virulent Y strain, but promoted the appearance of positive blood eultures in animais vaecinated and treated previously with the drug. Negative results are obtained if the immunosupressive agent is used 15 days after the vaccine. The flagellates isolated from the vaccinated Vbl treated mice remained avirulent for baby mice, after i. p. inoculation.

The immunity induced by the PF strain is probably sterile i. e. does not imply in the persistence of a latent infection (premunition).

RESUMO

Drogas imunossupressoras (Azathioprina e Methotrexato) que se mostraram capazes de agravar infecções experimentais por Trypanosoma cruzi virulento (cepa Y), em camundongos, não conseguiram favorecer o aparecimento de qualquer sinal de infecção em animais vacinados com a cepa avirulenta PF do mesmo tripanosoma.

Vinblastine, quando dado 3 dias antes da vacina, não favoreceu parasitemias positivas nem mortalidade significativamente aumentada, mas induziu o aparecimento de hemoculturas positivas.

Os tripanosomas dessas culturas foram incapazes de infectar camundongos jovens. Quando o Vinblastine foi usado 15 dias após a vacinação dos animais, todos os testes foram negativos, inclusive as hemoculturas.

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BIBLIOGRAPHY

Submitted to publication on 10.6.1971.

1. CAMARGO, M. E.; KLOETZEL, J. & BACHELLA, T. - A note on the haversting of large number of T. cruzi blood forms in adults dogs. Rev. Inst. Med. trop. São Paulo, 12: 217-220, 1970.
2. LUCKINS, A. G. - The effect of antilymphocyte serum and cyclophosphamide on the course of infection of T. brucei and T. congolense in rats and mice. Trans. Roy. Soc. Trop. Med. Hyg., 63: 423-424, 1969.
3. MENEZES, H. - Protective effect of an avirulent (cultivated) strain of Trypanosoma cruzi against experimental infection in mice. Rev. Inst. Med. trop. São Paulo, 10: 1-4, 1968.
4. MENEZES, H. - Lesões histológicas em camundongos "vacinados" com uma cepa avirulenta de Trypanosoma cruzi Rev. Bras. Med., 25: 160-165, 1968.
5. MENEZES, H. - Active immunization of mice with the avirulent Y strain of Trypanosoma cruzi against heterologous virulent strains of the same parasite. Rev. Inst. Med. trop. São Paulo, 11: 335-342, 1969.
6. MENEZES, H. - Active immunization of dogs with a non virulent strain of Trypanosoma cruzi Rev. Inst. Med. trop. São Paulo, 11: 258-263, 1969.
7. MENEZES, H. - I - The avirulence of the cultivated Y strain of Trypanosoma cruzi Rev. Inst. Med. trop. São Paulo, 12: 64-68, 1970.
8. MENEZES, H. - II - The avirulence of the cultivated Y strain of Trypanosoma cruzi Rev. Inst. Med. trop. São Paulo, 12: 129-135, 1970.
9. MENEZES, H. - III - The avirulence of the cultivated Y strain of Trypanosoma cruzi Rev. Inst. Med. trop. São Paulo, 13: 14-17, 1971.
10. MENEZES, H. & ALBUQUERQUE, R. - Imunização de camundongos com "vacina" viva avirulenta de Trypanosoma cruzi II - Variação do meio de cultura. Rev. Soc. Brasil. Med. Trop., 4: 69-74, 1970.
11. OKUMURA, M. & DÉCOURT, L. V. - Estudo de efeitos da administração de drogas imunodepressoras sôbre a Moléstia de Chagas experimental. Rev. Hosp. Clin. Fac. Med. S. Paulo, 24: 335-342, 1969.
12. SQUAME, G.; PANE, G; VISCONTI, M. & PIAZZA, M. - Attivitá di un farmaco immunosoppressore (azatioprina) sulla evoluzione delia epatite sperimentale de virus MHV3. Riv. Inst. Sieroter. Ital., 43: 251-256, 1968.
13. TRIPATHY, S. P. & MACKANESS, G. p. - The effect of cytotoxic agents on the primary immune response to Listeria monocytogenes J. Exp. Med., 13: 1-16, 1969.
14. WALKER, P. J. - The virulence of infections of P. berghei and T. rhodesiense in animais whose immune response has been impaired by radiation, drugs Or antilymphocyte serum. J. Protozool. 15: 33-, 1968 - Supl.
15. ZLOTNIK, I.; SMITH, C. E. G.; GRANT, D. P. & PEACOCK, S. - The effect of immuno suppression on viral encephalitis, with special reference to cyclophosphamide. Brit. J. exp. Path., 51: 434-439, 1970.

Datas de Publicação

Publicação nesta coleção
30 Jan 2013
Data do Fascículo
Ago 1971

Histórico

Aceito
10 Jun 1971
Recebido
10 Jun 1971

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[1] 1. CAMARGO, M. E.; KLOETZEL, J. & BACHELLA, T. - A note on the haversting of large number of T. cruzi blood forms in adults dogs. Rev. Inst. Med. trop. São Paulo, 12: 217-220, 1970.

[2] 2. LUCKINS, A. G. - The effect of antilymphocyte serum and cyclophosphamide on the course of infection of T. brucei and T. congolense in rats and mice. Trans. Roy. Soc. Trop. Med. Hyg., 63: 423-424, 1969.

[3] 3. MENEZES, H. - Protective effect of an avirulent (cultivated) strain of Trypanosoma cruzi against experimental infection in mice. Rev. Inst. Med. trop. São Paulo, 10: 1-4, 1968.

[4] 4. MENEZES, H. - Lesões histológicas em camundongos "vacinados" com uma cepa avirulenta de Trypanosoma cruzi Rev. Bras. Med., 25: 160-165, 1968.

[5] 5. MENEZES, H. - Active immunization of mice with the avirulent Y strain of Trypanosoma cruzi against heterologous virulent strains of the same parasite. Rev. Inst. Med. trop. São Paulo, 11: 335-342, 1969.

[6] 6. MENEZES, H. - Active immunization of dogs with a non virulent strain of Trypanosoma cruzi Rev. Inst. Med. trop. São Paulo, 11: 258-263, 1969.

[7] 7. MENEZES, H. - I - The avirulence of the cultivated Y strain of Trypanosoma cruzi Rev. Inst. Med. trop. São Paulo, 12: 64-68, 1970.

[8] 8. MENEZES, H. - II - The avirulence of the cultivated Y strain of Trypanosoma cruzi Rev. Inst. Med. trop. São Paulo, 12: 129-135, 1970.

[9] 9. MENEZES, H. - III - The avirulence of the cultivated Y strain of Trypanosoma cruzi Rev. Inst. Med. trop. São Paulo, 13: 14-17, 1971.

[10] 10. MENEZES, H. & ALBUQUERQUE, R. - Imunização de camundongos com "vacina" viva avirulenta de Trypanosoma cruzi II - Variação do meio de cultura. Rev. Soc. Brasil. Med. Trop., 4: 69-74, 1970.

[11] 11. OKUMURA, M. & DÉCOURT, L. V. - Estudo de efeitos da administração de drogas imunodepressoras sôbre a Moléstia de Chagas experimental. Rev. Hosp. Clin. Fac. Med. S. Paulo, 24: 335-342, 1969.

[12] 12. SQUAME, G.; PANE, G; VISCONTI, M. & PIAZZA, M. - Attivitá di un farmaco immunosoppressore (azatioprina) sulla evoluzione delia epatite sperimentale de virus MHV3. Riv. Inst. Sieroter. Ital., 43: 251-256, 1968.

[13] 13. TRIPATHY, S. P. & MACKANESS, G. p. - The effect of cytotoxic agents on the primary immune response to Listeria monocytogenes J. Exp. Med., 13: 1-16, 1969.

[14] 14. WALKER, P. J. - The virulence of infections of P. berghei and T. rhodesiense in animais whose immune response has been impaired by radiation, drugs Or antilymphocyte serum. J. Protozool. 15: 33-, 1968 - Supl.

[15] 15. ZLOTNIK, I.; SMITH, C. E. G.; GRANT, D. P. & PEACOCK, S. - The effect of immuno suppression on viral encephalitis, with special reference to cyclophosphamide. Brit. J. exp. Path., 51: 434-439, 1970.

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The avirulence of the cultivated Y strain of Trypanosoma cruzi: IV. The effect of immunosuppressive agents in mice

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