CHROMATOGRAPHIC INVESTIGATION OF RUTHENIUM NITROSYL COMPLEX: NO INTERCONVERSION AND REACTIONS WITH BIOLOGICAL REDUCTANTS

Abstract

One experimental strategy to prepare a nitrosyl metal complex is based on the acid-base conversion of NO₂^- into NO⁺. Here, we employed UV-vis absorption and FTIR spectroscopies to investigate the reaction of cis-[Ru(NO₂)(bpy)₂(imN)]PF₆ with H₃O⁺, which produced cis-[Ru(NO)(bpy)₂(imN)](PF₆)₃ complex. Chromatographic studies were carried out and showed that immediately after nitrite complex was dissolved only one species was present with retention time(t_R) of 6.81 minutes. Addition of H₃O⁺ to nitrite complex led to the formation of one major peak with t_R of 3.92 min supporting nitrosyl complex formation. The reaction of nitrosyl complex with cysteine was also monitored by HPLC and it showed clearly the formation and followed decrease of a peak at 3.38 minute with maximum absorption at 380 nm, consistent with an intermediate complex. Later, it was observed the appearance of a peak at 4.15 minute with absorption band at 470 nm. In contrast to the reaction with cysteine, methionine did not show the formation of any intermediate. The use of HPLC was an important tool to support mechanistic assumptions for nitrosyl reactions.

Keywords:
nitrite/nitrosyl interconversion; ruthenium; cysteine and HPLC

INTRODUCTION

For over three decades, nitric oxide effects on biological systems have been studied revealing many important roles and medical implications.^1-6 Due to this, ruthenium nitrosyl complexes have emerged as an efficient system for the design of NO donors with potential pharmacological applications.^7-10 The most common synthetic route to prepare ruthenium nitrosyl complexes is based on the conversion of coordinated NO₂^- into NO⁺.¹⁰ However, as far as we are concerned, a careful monitoring of this reaction using HPLC has never been reported in literature.

The reactivity, photochemical and stereochemical properties of ruthenium bipyridine complexes have been increasingly investigated.^10-20 These studies were carried out mainly using spectroscopic and electrochemical techniques. However, these techniques can have some drawbacks, based on the fact they might not detect small amount of impurities neither better describe composition of a mixture of species. Despite these advantages, there are not many cases reported in the literature where the reactivity of metal complexes was investigated using HPLC. Nevertheless, the study of reactivity of ruthenium bipyridine complexes can be a suitable example for the use of HPLC. Identification of an intermediate along with optimization of new synthetic route were described for cis-[Ru(bpy)₂(L)₂]²⁺ complex,²¹ along with a few other cases.^22-25

However, many other cases have not taken advantage of this technique to better address more complex problems and separations as well. For example, the trans-[Ru(bpy)₂(XY)₂]²⁺ compounds are prepared by photoisomerization of cis-[Ru(bpy)₂(H₂O)₂]²⁺ following binding of X and Y ligands.²⁶ This photoreaction is still quite inefficient and very often difficult to obtain complexes with high purity.

Another case is related to the nitrosyl/nitrite equilibrium in a metal complex, which is dependent on the pH as described below (Equation 1).^27-34 These species exhibit quite distinct overall charge, which can strongly alter interaction with reverse phase column.

(1) ${\left[\mathrm{Ru}\left(\mathrm{NO}\right){\left(\mathrm{bpy}\right)}_{2}L\right]}^n+{2OH}^{-}\rightleftharpoons {\left[\mathrm{Ru}\left({\mathrm{NO}}_2\right){\left(\mathrm{bpy}\right)}_{2}L\right]}^{n-2}+H_{2}O$

Sometimes nitrosyl complexes of the type [Ru(NO)(bpy)₂L]³⁺ can be easily converted into the nitrite form even at low pH.³⁵ Thus it is necessary to work in a highly acidic medium to shift the equilibrium to the nitrosyl form.

The reactions with thiolates are more complicated than with OH^-, because irreversible processes occur with subsequent adduct formation. Some reactions of nucleophiles with nitrosyl complexes have been reviewed,^36-40 leading to the formation of gas (N₂ or N₂O) as result of the adduct decompositions. Intermediate adducts have showed strong absorption around 520 nm as noticed during a fast reactions of nitroprusside and diverse thiolates,^41-43 leading to the reduction of nitric oxide and the oxidation of the thiolates.^44-49

In this work we report the monitoring of the reaction involving the nitrite/nitrosyl interconversion and sulfur-based compounds with cis-[Ru(NO)(bpy)₂(imN)]³⁺. These studies can validate the suitable use of HPLC for better assignment of complex reactions and help in the design of new nitric oxide releasing complexes.

EXPERIMENTAL

Chemicals and reagents

Imidazole (99%, Aldrich) was used without any further treatment. Ruthenium trichloride (RuCl₃·nH₂O) (37 to 42% Ru, Aldrich) was used in the synthesis of cis-[RuCl₂(bpy)₂]. This latter complex was synthesized using a method previously described in literature.^50,51 Other reagents and solvents of analytical grade were used as purchased.

Synthesis of the complex cis-[Ru(NO₂)(bpy)₂(imN)]PF₆ (1)

This compound was prepared dissolving cis-[RuCl₂(bpy)₂] (0.200 g, 0.41 mmol) and imidazole (ImN) (0.033 g, 0.49 mmol) in 20 ml of a solution prepared by the ethanol-water mixture. The reactional system was kept under reflux for 2 h. After this period, sodium nitrite (0.035 g, 0.49 mmol) was added to the solution and allowed to react under reflux for more 2 h. The solid was obtained after addition of saturated ammonium hexafluorophosphate in aqueous solution (1.0 mL). Yield: 0.212 g, 76%. C₂₃H₁₉F₆N₇O₂PRu (672.36): calcd. C 41.06%, H 2.81%, N 14.59%; found C 41.22%, H 2.78%, N 14.49%. These data were obtained in a Perkin-Elmer CHN 2400 analyzer.

Monitoring the nitrite/nitrosyl interconversion by High-Performance Liquid Chromatography

The chromatographic analysis was performed on a Shimadzu liquid chromatograph equipped with a LC-10AD pump and SPD-M10A UV-Visible photodiode-array detector with a CBM-10AD interface. An Alltech ODS reverse-phase column (250 mm x 4.6 mm, 5 µm particle) was used under isocratic elution regime with 15:85 acetonitrile-water solution containing 0.1% trifluoroacetic acid, pH = 3.5. The chromatograms were obtained at a constant flow-rate of 1.0 mL min^-1 after injecting 5 µL of the solution, all monitoring was reported using a wavelength of 300 nm.

The measurements were obtained using a 100 mL of cis-[Ru(NO₂)(bpy)₂(imN)]⁺ aqueous solution at 5 x 10^-5 mol.L^-1 in pH = 8.0 and temperature of 25 ºC. Then pH was changed by addition of trifluoroacetic acid and maintaining the solution under constant agitation for five minutes before each injection.

After the nitrite complex was completely consumed as indicated by the area of the peak at 6.81 minutes, the volume of that solution was reduced to 10 mL and the complex cis-[Ru(NO)(bpy)₂(imN)]³⁺ (2) was isolated and characterized by UV-Vis, Infrared, NMR spectroscopies and electrochemical techniques. The interconversion from Ru-NO to Ru-NO₂^- was also monitored by Uv-Vis spectroscopy as previously described.³³

Electronic spectra were recorded in aqueous solution at room temperature on a UV-Vis, HP-8453 diode-array spectrophotometer. Vibrational spectra in the infrared region were obtained in potassium bromide pellets. The analysis was performed using an infrared spectrophotometer Shimadzu IR Prestige-21. The electrochemical measurements were performed in aqueous solution of NaTFA 0.1 mol L^-1, pH = 3.4, using the Ag/AgCl (Epsilon potentiostat from Bioanalytical Systems) as reference electrode.

Monitoring the reactions of nitrosyl complex with cysteine and methionine

These chromatographic analysis were conducted mixing the complex 1 at 5.1 x 10^-5 mol L^-1 with the sulfur-based reductants, cysteine or methionine at 5.1 x 10^-4 mol L^-1, in an sodium acetate buffer pH 3.4, ionic strength of 0.1 mol L^-1.

A 15-fold ratio of cysteine or methionine over the concentration of the complex was kept to ensure a pseudo first-order condition. This reaction was monitored by HPLC with injections of 5 µL of the mixture every 10 minutes, and temperature kept at 25 ºC.

RESULTS AND DISCUSSION

Monitoring the nitrite/nitrosyl interconversion

The nitrosyl complex, cis-[Ru(NO)(bpy)₂(imN)]³⁺ (compound 2), was obtained by acidification of the precursor cis-[Ru(NO₂)(bpy)₂(imN)]⁺ solution (compound 1). This acid-base reaction is presented in Equation 2.

(2) $\mathrm{cis}-\left[\mathrm{Ru}\left({\mathrm{NO}}_2\right){\left(\mathrm{bpy}\right)}_2\left(\mathrm{imN}\right)\right]^{+}{}_{\left(\mathrm{aq}\right)}+H_2{O}_{\left(l\right)}\underset{k_{-1}}{\overset{k_1}{\rightleftharpoons }}\mathrm{cis}-\left[\mathrm{Ru}\left(\mathrm{NO}\right){\left(\mathrm{bpy}\right)}_2\left(\mathrm{imN}\right)\right]^{3+}{}_{\left(\mathrm{aq}\right)}+{2OH}_{\left(\mathrm{aq}\right)}^{-}$

Electronic absorption study of this reaction showed one single inflection dependent of pH (Figure 1), indicating a equilibrium of interconversion described in Equation 2. As the pH of the solutions containing these complexes is increased, new intense bands with a maximum in the 400-450 nm range are observed. These bands can be assigned to the (bpy)π* ← dπ(Ru) MLCT transition of the nitro species formed according to the chemical equilibrium displayed. The determination of the both species present in equal concentrations for Equation 2 was carried out using the spectrophotometric method (absorbance measurements were performed at 410 nm). The total ionic strength was kept at 0.5 mol L^-1 with sodium trifluoroacetate, according to similar studies reported for the Ru^II33 systems. The pKa for this process was determined as 5.54, which is close to the value measured for other similar nitrosyl complexes having bipyridine and auxiliary nitrogen-bound ligands.³³

Figure 1
Spectrophotometric monitoring of the interconversion of cis-[Ru(NO)(bpy)₂(imN)]³⁺ into cis-[Ru(NO₂)(bpy)₂(imN)]⁺ at different pH values (λ_max = 410 nm; NaTFA aqueous solution, µ = 1.0 mol.L^-1)

The chromatogram of 1 at pH 8.0 showed only one peak with retention time of 6.81 minutes (Figure 2). The absence of any additional peak indicated the stability of the nitrite ion coordinated to the Ru(II) metal in that pH. However, upon a gradual decrease of the pH of the solution until pH 3.5, the chromatographic profile changed dramatically (Figure 2). Already at pH 6.5, the area of the peak corresponding to compound 1 decreased and additional peaks with lower retention time were observed.

Figure 2
HPLC monitoring of the interconversion reaction of nitrite/nitrosyl, at 300 nm. Figure 2a showed the chromatogram of cis-[Ru(NO₂)(bpy)₂(imN)]⁺ (compound 1, t_R = 3.92 min) and cis-[Ru(NO)(bpy)₂(imN)]³⁺ (compound 2, t_R = 6.81 min) complexes ions, with their respective UV-Vis spectra. (b) UV-Vis spectrum of the cis-[Ru(NO₂)(bpy)₂(imN)]⁺ (1) and cis-[Ru(NO)(bpy)₂(imN)]³⁺ (2) as obtained from HPLC. (c) Profile for the reaction of 1 with H⁺ as monitored by HPLC: black squares (relative area for peak of 3.92 minutes) and black circles (relative area for peak of 6.81 minutes)

Of course, the rate of this reaction is extremely pH dependent, and at very low pH (pH < 3.0), k₁ is very high. For the reverse reaction (k_-1) where there is a hydroxide attack, this reaction rate follows the opposite trend, i.e. higher rate constant at high pH values.^27,52,53 The detection of the chemical specie formed in nitrite complex solution when it is submitted to different pH conditions was monitored by HPLC (Figure 2).

Figure 2a showed the chromatogram of cis-[Ru(NO₂)(bpy)₂(imN)]⁺ (compound 1, t_R = 3.92 min) and cis-[Ru(NO)(bpy)₂(imN)]³⁺ (compound 2, t_R = 6.81 min) complexes ions, with their respective UV-Vis spectra. There are strong differences in their electronic spectra and retention times, supporting the appropriated use of HPLC. This is a reasonable behavior since the charge of the nitrosyl complex was higher than the nitrite complex. Thus, it is rational to expect the nitrosyl complex exhibited a more polar character than the nitrite one. So, the former would be less retained on the column, consequently, showing a shorter retention time. Interestingly, in addition to the main peaks corresponding to nitro and nitrite complexes, other peaks with low intensity were observed, which had been assigned to possible intermediates formed during the interconversion process. This behavior was reported by other kinetic studies indicating multiple steps.^{21-24,31-33,35}

There is an efficient consumption of cis-[Ru(NO₂)(bpy)₂(imN)]⁺ with concomitant production of cis-[Ru(NO)(bpy)₂(imN)]³⁺ species, as indicated in the chromatogram along with the identification of these species by their UV-vis spectra (Figure 2b). After addition of H₃O⁺, the major peak was at 3.92 min corresponding to compound 2 as shown by the chromatograms (Figure 2a).

The electrophilic reactions of the NO ligand bound to transition metal centers have long been study and constitute one of the important reactivity properties for these compounds.^29,34 It has been reported that when solutions of {MNO}⁶ complexes are made alkaline,^36,40 a product with absorption band from 300 - 400 nm is observed.^31,52,53 Additionally, this transformation is fully reversible upon addition of acid. Currently, there is no debate regarding the nature of this reaction corresponding to the interconversion of the nitrosyl complex into the nitrite.^31,52,53 This resulted by a nucleophilic attack of the hydroxyl into the NO⁺ group, and a new band observed in 400 nm may be assigned to a metal to ligand charge transfer from Ru(II) to the NO₂^- ligand.^31,52

Based on kinetics studies,^31,52-54 a series of step reactions have been proposed to explain the pathways of the nitrite/nitrosyl interconversion as described below.

(3) $\mathrm{cis}-{\left[\mathrm{Ru}\left({\mathrm{NO}}_2\right){\left(\mathrm{bpy}\right)}_2\left(\mathrm{imN}\right)\right]}_{\left(\mathrm{aq}\right)}^{+}+H_2{O}_{\left(l\right)}\stackrel{k_2}{\rightleftharpoons }\mathrm{cis}-\left[\mathrm{Ru}\left({\mathrm{NO}}_{2}H\right){\left(\mathrm{bpy}\right)}_2\left(\mathrm{imN}\right)\right]^{2+}{}_{\left(\mathrm{aq}\right)}+{\mathrm{OH}}_{\left(\mathrm{aq}\right)}^{-}$

(4) $\mathrm{cis}-{\left[\mathrm{Ru}\left({\mathrm{NO}}_{2}H\right){\left(\mathrm{bpy}\right)}_2\left(\mathrm{imN}\right)\right]}_{\left(\mathrm{aq}\right)}^{2+}\stackrel{k_3}{\rightleftharpoons }\mathrm{cis}-{\left\{\left[\mathrm{Ru}\left(\mathrm{NO}\right){\left(\mathrm{bpy}\right)}_2\left(\mathrm{imN}\right){.OH}^{-}\right]\right\}}_{\left(\mathrm{aq}\right)}^{2+}$

(5) $\mathrm{cis}-{\left\{\left[\mathrm{Ru}\left(\mathrm{NO}\right){\left(\mathrm{bpy}\right)}_2\left(\mathrm{imN}\right)\right]{.OH}^{-}\right\}}_{\left(\mathrm{aq}\right)}^{2+}\stackrel{k_4}{\rightleftharpoons }\mathrm{cis}-\left[\mathrm{Ru}\left(\mathrm{NO}\right){\left(\mathrm{bpy}\right)}_2\left(\mathrm{imN}\right)\right]^{3+}{}_{\left(\mathrm{aq}\right)}+\mathrm{OH}^{-}{}_{\left(\mathrm{aq}\right)}$

Thus, it is reasonable to conclude that the extra small peaks observed during the chromatograms could be due to actual intermediates as proposed in the above mechanism.

Figure 2c shows the equilibrium plot as a function of pH for the reaction of nitrite/nitrosyl interconversion. The graph shows significant correlation between a decrease in peak attributed to the compound 1 and the appearance of the peak referring of the compound 2 as a result of the addition of H₃O⁺. This profile is due to an equilibrium of these species (NO⁺/NO₂^-). The use of HPLC proved to be a great support for monitoring this reaction and identifying species involved in the reaction of nitrite/nitrosyl interconversion. Additionally, it indicated also a great similarity in the pKa value calculate by UV-visible spectroscopy (pKa = 5.54),³³ reinforcing the possibility to determine this parameter using HPLC.

Aiming to assure the nature of the final product after reaching the lowest pH, this compound was isolated by addition of a saturated solution of NH₄PF₆ and characterized by UV-Vis, FTIR and electrochemical techniques. This product showed all features found for cis-[Ru(NO)(bpy)₂(imN)]³⁺ complex, such as electronic spectrum in water showing an intense band at 290 nm (2.45 x 10⁴L mol^-1 cm^-1) attributed to the ligand π*← π (bpy) transition.^55,56 Also, another absorption band at 323 nm (8.92 x 10³ L mol^-1 cm^-1) was observed attributed to MLCT, (bpy) π*←dp(Ru). For this type of complex, one absorption is also expected due to (NO⁺)π*←dp(Ru). Armor and Pell⁵⁷ observed this transition for complexes of the type cis-[Ru(NO)(NH₃)₄X]⁺ⁿ, where X = OH^-, Cl^-, Br^-, or I^- and n = 2; or X = H₂O and n = 3) with ε ~ 50 L. mol^-1 cm^-1. For ruthenium bipyridine complexes, this band is observed with a higher energy than in the case of tetraamines due to the stabilization of the dp(Ru) orbitals caused by the ligands. Thus, in the cis-[Ru(NO)(bpy)₂(imN)](PF₆)₃ this band is probably overlapped by the intense band at 323 nm.

Additionally, it was found a very intense peaks in the FTIR spectrum at 1944 cm^-1 assigned to the νNO⁺ vibrational mode characteristic of similar systems.^10,30,58-61 The cis-[Ru(NO)(bpy)₂(imN)](PF₆)₃ complex cyclic voltammetry obtained in 0.1 mol L^-1 reveals only reversible electrochemical process characteristic of the nitrosyl ligand, also noticed for the isolated compound.

Monitoring the reaction of the nitrosyl complex with cysteine and methionine

The nucleophilic reactions promoted by thiols have been studied for a series of ruthenium nitrosyl complexes.^34,62 The rate of this reaction is also extremely dependent of the concentration of H₃O⁺, whereas at higher pH the reaction occurs faster. Due to this, we decided to work at pH 3.5, since it would slow down the reaction and make possible to follow the products by HPLC. Additionally, at this pH we can assure the complex is in a nitrosyl form.

The chromatogram corresponding to the reaction of the cis-[Ru(NO)(bpy)₂(imN)]³⁺ complex with cysteine can be observed in Figure 3a. In the first chromatogram, before cysteine addition, there was only one major peak at 2.72 minutes retention time whose electronic spectra was also consistent with compound 2. Interestingly, right after the addition of cysteine, it was observed formation of a new species (compound 3) with peak at 3.38 minutes. The electronic spectrum of this compound 3 (Figure 3b) showed band at 380 nm, which is consistent with the same intermediate I₂ (cis-[Ru(N(O)(SR)₂)(bpy)₂(imN)]⁺) described by stopped-flow studies by Silva et al.⁶³

Figure 3
Monitoring the reaction of cis-[Ru(NO)(bpy)₂(imN)]³⁺ with cysteine using HPLC. (a) Chromatograms for the reaction carried out in 0.1 mol. L^-1 acetate buffer pH 3.5. (b) UV-Vis spectra of cis-[Ru(NO)(bpy)₂(imN)]³⁺ (2), peak at retention time 2.72 min, cis-[Ru(N(O)RS)₂(bpy)₂(imN)]⁺ (3) at 3.38 min and cis-[Ru(bpy)₂(imN)(H2O)]²⁺ (4) at 4.15 min. (c) Kinetic profile using peak area for each species, black squares (at 2.72 min), stars (at 3.38 min) and black triangles (at 4.15 min)

Later on, another chromatographic peak at 4.15 minutes was observed, assigned to compound 4. This latter showed a metalto-ligand charge transfer (MLCT) transition band, dp(Ru)← π* (bipyridine), with maximum at 470 nm, which indicated formation of the cis-[Ru(bpy)₂(imN)(H₂O)]²⁺ complex (Figure 3b).³³ The intense electronic transition bands observed upon addition of cysteine to sodium nitroprusside (NP)⁶² and also to the complex investigated here can be described as due to MLCT of the {(X)₅MN(O)SR} adducts. These results corroborated with the proposed kinetic mechanism.⁶³

Based on kinetic studies,^23,35,63 the first step of this reaction is the formation of the intermediate I₁ which occured very fast and we were not able to measure the rate constant.⁶² Therefore, our study was unable to monitor I₁ (cis-[Ru(N(O)(SR))(bpy)₂(imN)]²⁺) formation.

Figure 3c shows the relative kinetic profile of the areas of the peaks of each species generated during this reaction. This result indicates a similar behavior as described by the kinetic study ever conducted for similar systems,^62,63 where there is formed an I₂ intermediate (compound 3) with the peak chromatogram being formed during 30 min and then there is a subsequent decay to the final product (compound 4) and NO⁰ release. The analysis monitored by HPLC there is a separation of the substances formed during the reaction enabling the identification of each species.

Roncaroli and Olabe⁶² proposed the following mechanism for the reaction of complex nitrosyl with cysteine:

$\begin{array}{c}\hfill {\left[{\left(X\right)}_5\mathrm{RuNO}\right]}^n\underset{-\mathrm{RS}-}{\overset{\mathrm{RS}-}{\rightleftharpoons }}{\left[\left.{\left(X\right)}_5\mathrm{RuN}\left(O\right)\mathrm{SR}\right)\right]}^{n-1}\underset{-\mathrm{RS}-}{\overset{\mathrm{RS}-}{\rightleftharpoons }}{\left[\left.{\left(X\right)}_5\mathrm{RuN}\left(O\right){\left(\mathrm{SR}\right)}_2\right)\right]}^{-n2}\\ \hfill \downarrow \\ \hfill {\left[{\left(X\right)}_5\mathrm{Ru}\left(H_{2}O\right)\right]}^{n-1}+\raisebox{1ex}{$1$}\!\left/ \!\raisebox{-1ex}{$2$}\right.N_{2}O+\mathrm{RSSR}\end{array}$

In a similar way, the reaction with the complex studied here can be described as occurring in three steps (Scheme 1) according:

Scheme 1
Reaction scheme of cis-[Ru(NO)(bpy)₂(imN)]³⁺ complex with cysteine monitored by HPLC

Aiming to investigate the role of this deprotonation in the reaction with nitrosyl complex, we decided to use methionine and monitored the reaction by HPLC. The chromatograms showed an initial peak at 2.31 minutes corresponding to the nitrosyl complex (cis-[Ru(NO)(bpy)₂(imN)](PF₆)₃, compound 2) that decreased over time while another peak at 3.67 minutes was formed. This latter peak was assigned to the ruthenium aqua complex (Scheme 1, compound 4), which may be formed due to the release of nitric oxide.

Interestingly, in these experiments, it was not observed the formation of any intermediate, as observed for the reaction with cysteine (Figure 4b). Once methionine presented a methyl group in place of hydrogen, the redox process happens without formation of the adducts (I₁ and compound 3). This result corroborated with kinetic data by stopped-flow,⁶³ which indicated formation of intermediates only using thiols and the nitric oxide accelerates the reaction rate.

Figure 4
Monitoring the reaction of cis-[Ru(NO)(bpy)₂(imN)]³⁺ with methionine using HPLC. (a) Chromatograms for the reaction carried out in 0.1 mol. L^-1 acetate buffer pH 3.5. (b) UV-Vis spectra of the peaks at retention times 2.31 min, cis-[Ru(NO)(bpy)₂(imN)]³⁺ (2) and 3.67 min, cis-[Ru(bpy)₂(imN)(H2O)]⁺ (4). (c) Kinetic profile for the reaction of cis-[Ru(NO)(bpy)₂(imN)]³⁺ complex ion with methionine monitored by HPLC: black squares (relative area for peak of 2.31 minutes) and black triangle (relative area for peak of 3.67 minutes)

CONCLUSIONS

Our results showed that HPLC can be used as a powerful technique to monitor and characterize more complex reactions involving metal complexes such as nitrite/nitrosyl interconversion in ruthenium bipyridine complexes. It was possible to follow and assigned products observed during the reaction of cis-[Ru(NO)(bpy)₂(imN)](PF₆)₃, with cysteine and methionine, and supported by other techniques to better describe a mechanism for these reactions. Nevertheless, HPLC was suitable to identify and monitor the formation and consumption of intermediate species of the reaction of thiols with nitrosyl complexes.

ACKNOWLEDGEMENTS

The authors acknowledge the Brazilian agencies CNPq, CAPES, and FUNCAP for the financial support: L. G. F. Lopes (CNPq 303732/2014-8; PPSUS 12535691-9), E. H. S. Sousa (PVE - 09/ 2014 process 88881.068193/2014-01, CNPq 312030/ 2015-0) and F. O. N. Silva (CNPq: 4425868/2014-2) are supported by the National Council for Scientific and Technological Development of Brazil.

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Publication Dates

• Publication in this collection
  Apr 2018

History

• Received
  22 Sept 2017
• Accepted
  06 Dec 2017