Criopreservação de sementes de <i>Adenium obesum</i> em nitrogênio líquido

O objetivo deste trabalho foi avaliar crioprotetores na qualidade fisiológica de sementes de Adenium obesum preservadas em nitrogênio líquido. Foram utilizados os seguintes tratamentos: T1, controle, sem crioprotetor e sem imersão em nitrogênio líquido; T2, com imersão em nitrogênio líquido e sem crioprotetor; T3, com 0,4 mol L^-1 de sacarose; T4, com 0,8 mol L^-1 de sacarose; T5, com 1,0 mol L^-1 de glicerol; T6, com 2,0 mol L^-1 de glicerol com PVS1; T7, com PVS1; T8, com PVS2; T9, com PVS2 + floroglucinol (PVS2 modificado) e T10, com PVS3. As sementes de Adenium obesum apresentam tolerância à imersão e ao armazenamento em nitrogénio líquido, e não necessitam de crioprotectores para manter a qualidade fisiológica quando conservadas em água a 7,5%.

Termos para indexação
Adenium obesum; crioprotetores; tolerância ao congelamento; germinação de sementes

Autoria

Talita Angélica de Oliveira Rosa

Universidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR, BrazilUniversidade Estadual de LondrinaBrazilLondrina, PR, BrazilUniversidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR, Brazil

https://orcid.org/0009-0005-5463-6682

Sérgio Pedro JuniorCorresponding author

https://orcid.org/0000-0003-1355-3168

Débora Perdigão Tejo

https://orcid.org/0000-0002-1998-8413

Jean Carlo Baudraz de Paula

https://orcid.org/0000-0002-3695-3386

Gabriel Danilo Shimizu

https://orcid.org/0000-0001-8524-508X

Ricardo Tadeu de Faria

https://orcid.org/0000-0002-7595-1965

Corresponding author

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Figuras

Figuras (2)

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Figure 1
Germination (A), mean velocity time (MVT) (B), germination velocity index (GVI) (C) of Adenium obesum seeds under the following treatments: T1, free from cryoprotectors and not immersed in liquid nitrogen; T2, free from cryoprotective substances; T3, sucrose 0.4 mol L^-1; T4, sucrose 0.8 mol L^-1; T5, glycerol 1 mol L^-1; T6, glycerol 2 mol L^-1; T7, PVS1 [19% glycerol (v/v), 13% ethylene glycol (v/v), 6% dimethylsulfanoxide (v/v)]; T8, PVS2 [30% glycerol (v/v), 15% ethylene glycol (v/v), 15% dimethylsulfanoxide (v/v)]; T9, PVS2 + 1% floroglucinol; T10, PVS3 [50% glycerol (v/v) and 50% sucrose (v/v)]. Averages followed by the same letter do not differ from each other by Scott-Knott’s test at 5% probability.

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Figure 2
Aerial part length (APL) (A), root length (RL) (B), aerial part dry mass (APDM) (C), and root dry mass (RM) (D) of Adenium obesum seeds under the following treatments: T1, free from cryoprotectors and not immersed in liquid nitrogen; T2, free from cryoprotective substances; T3, sucrose 0.4 mol L^-1; T4, sucrose 0.8 mol L^-1; T5, glycerol 1 mol L^-1; T6, glycerol 2 mol L^-1; T7, PVS1 [19% glycerol (v/v), 13% ethylene glycol (v/v), 6% dimethylsulfanoxide (v/v)]; T8, PVS2 [30% glycerol (v/v), 15% ethylene glycol (v/v), 15% dimethylsulfanoxide (v/v)]; T9, PVS2 + 1% floroglucinol; T10, PVS3 [50% glycerol (v/v) and 50% sucrose (v/v)]. Note: Except for T1, in all other treatments, the seeds were immersed in liquid nitrogen. Averages followed by the same letter do not differ from each other by the Scott-Knott’s test at 5% probability.

Figure 1 Germination (A), mean velocity time (MVT) (B), germination velocity index (GVI) (C) of Adenium obesum seeds under the following treatments: T1, free from cryoprotectors and not immersed in liquid nitrogen; T2, free from cryoprotective substances; T3, sucrose 0.4 mol L^-1; T4, sucrose 0.8 mol L^-1; T5, glycerol 1 mol L^-1; T6, glycerol 2 mol L^-1; T7, PVS1 [19% glycerol (v/v), 13% ethylene glycol (v/v), 6% dimethylsulfanoxide (v/v)]; T8, PVS2 [30% glycerol (v/v), 15% ethylene glycol (v/v), 15% dimethylsulfanoxide (v/v)]; T9, PVS2 + 1% floroglucinol; T10, PVS3 [50% glycerol (v/v) and 50% sucrose (v/v)]. Averages followed by the same letter do not differ from each other by Scott-Knott’s test at 5% probability.

Figure 2 Aerial part length (APL) (A), root length (RL) (B), aerial part dry mass (APDM) (C), and root dry mass (RM) (D) of Adenium obesum seeds under the following treatments: T1, free from cryoprotectors and not immersed in liquid nitrogen; T2, free from cryoprotective substances; T3, sucrose 0.4 mol L^-1; T4, sucrose 0.8 mol L^-1; T5, glycerol 1 mol L^-1; T6, glycerol 2 mol L^-1; T7, PVS1 [19% glycerol (v/v), 13% ethylene glycol (v/v), 6% dimethylsulfanoxide (v/v)]; T8, PVS2 [30% glycerol (v/v), 15% ethylene glycol (v/v), 15% dimethylsulfanoxide (v/v)]; T9, PVS2 + 1% floroglucinol; T10, PVS3 [50% glycerol (v/v) and 50% sucrose (v/v)]. Note: Except for T1, in all other treatments, the seeds were immersed in liquid nitrogen. Averages followed by the same letter do not differ from each other by the Scott-Knott’s test at 5% probability.

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Criopreservação de sementes de <i>Adenium obesum</i> em nitrogênio líquido

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[1] Corresponding author