Cryopreservation of <i>Adenium obesum</i> seeds in liquid nitrogen

The objective of this work was to evaluate cryoprotectants on the physiological quality of Adenium obesum seeds preserved in liquid nitrogen. The following treatments were used: T1, control, without cryoprotectant and without immersion in liquid nitrogen; T2, with immersion in liquid nitrogen and without cryoprotectant; T3, with 0. 4 mol L^-1 sucrose; T4, with 0.8 mol L^-1 sucrose; T5, with 1.0 mol L^-1 glycerol; T6, with 2.0 mol L^-1 glycerol with PVS1; T7, with PVS1; T8, with PVS2; T9, with PVS2 + phloroglucinol (modified PVS2); and T10, with PVS3. Adenium obesum seeds show tolerance to immersion and storage in liquid nitrogen and do not need cryoprotectants to maintain their physiological quality when stored in a water content of 7.5%.

Index terms
Adenium obesum; cryoprotectants; freezing tolerance; seed germination

Authorship

Talita Angélica de Oliveira Rosa

Universidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR, BrazilUniversidade Estadual de LondrinaBrazilLondrina, PR, BrazilUniversidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR, Brazil

https://orcid.org/0009-0005-5463-6682

Sérgio Pedro JuniorCorresponding author

https://orcid.org/0000-0003-1355-3168

Débora Perdigão Tejo

https://orcid.org/0000-0002-1998-8413

Jean Carlo Baudraz de Paula

https://orcid.org/0000-0002-3695-3386

Gabriel Danilo Shimizu

https://orcid.org/0000-0001-8524-508X

Ricardo Tadeu de Faria

https://orcid.org/0000-0002-7595-1965

Corresponding author

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Figures (2)

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Figure 1
Germination (A), mean velocity time (MVT) (B), germination velocity index (GVI) (C) of Adenium obesum seeds under the following treatments: T1, free from cryoprotectors and not immersed in liquid nitrogen; T2, free from cryoprotective substances; T3, sucrose 0.4 mol L^-1; T4, sucrose 0.8 mol L^-1; T5, glycerol 1 mol L^-1; T6, glycerol 2 mol L^-1; T7, PVS1 [19% glycerol (v/v), 13% ethylene glycol (v/v), 6% dimethylsulfanoxide (v/v)]; T8, PVS2 [30% glycerol (v/v), 15% ethylene glycol (v/v), 15% dimethylsulfanoxide (v/v)]; T9, PVS2 + 1% floroglucinol; T10, PVS3 [50% glycerol (v/v) and 50% sucrose (v/v)]. Averages followed by the same letter do not differ from each other by Scott-Knott’s test at 5% probability.

Thumbnail

Figure 2
Aerial part length (APL) (A), root length (RL) (B), aerial part dry mass (APDM) (C), and root dry mass (RM) (D) of Adenium obesum seeds under the following treatments: T1, free from cryoprotectors and not immersed in liquid nitrogen; T2, free from cryoprotective substances; T3, sucrose 0.4 mol L^-1; T4, sucrose 0.8 mol L^-1; T5, glycerol 1 mol L^-1; T6, glycerol 2 mol L^-1; T7, PVS1 [19% glycerol (v/v), 13% ethylene glycol (v/v), 6% dimethylsulfanoxide (v/v)]; T8, PVS2 [30% glycerol (v/v), 15% ethylene glycol (v/v), 15% dimethylsulfanoxide (v/v)]; T9, PVS2 + 1% floroglucinol; T10, PVS3 [50% glycerol (v/v) and 50% sucrose (v/v)]. Note: Except for T1, in all other treatments, the seeds were immersed in liquid nitrogen. Averages followed by the same letter do not differ from each other by the Scott-Knott’s test at 5% probability.

Figure 1 Germination (A), mean velocity time (MVT) (B), germination velocity index (GVI) (C) of Adenium obesum seeds under the following treatments: T1, free from cryoprotectors and not immersed in liquid nitrogen; T2, free from cryoprotective substances; T3, sucrose 0.4 mol L^-1; T4, sucrose 0.8 mol L^-1; T5, glycerol 1 mol L^-1; T6, glycerol 2 mol L^-1; T7, PVS1 [19% glycerol (v/v), 13% ethylene glycol (v/v), 6% dimethylsulfanoxide (v/v)]; T8, PVS2 [30% glycerol (v/v), 15% ethylene glycol (v/v), 15% dimethylsulfanoxide (v/v)]; T9, PVS2 + 1% floroglucinol; T10, PVS3 [50% glycerol (v/v) and 50% sucrose (v/v)]. Averages followed by the same letter do not differ from each other by Scott-Knott’s test at 5% probability.

Figure 2 Aerial part length (APL) (A), root length (RL) (B), aerial part dry mass (APDM) (C), and root dry mass (RM) (D) of Adenium obesum seeds under the following treatments: T1, free from cryoprotectors and not immersed in liquid nitrogen; T2, free from cryoprotective substances; T3, sucrose 0.4 mol L^-1; T4, sucrose 0.8 mol L^-1; T5, glycerol 1 mol L^-1; T6, glycerol 2 mol L^-1; T7, PVS1 [19% glycerol (v/v), 13% ethylene glycol (v/v), 6% dimethylsulfanoxide (v/v)]; T8, PVS2 [30% glycerol (v/v), 15% ethylene glycol (v/v), 15% dimethylsulfanoxide (v/v)]; T9, PVS2 + 1% floroglucinol; T10, PVS3 [50% glycerol (v/v) and 50% sucrose (v/v)]. Note: Except for T1, in all other treatments, the seeds were immersed in liquid nitrogen. Averages followed by the same letter do not differ from each other by the Scott-Knott’s test at 5% probability.

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Cryopreservation of <i>Adenium obesum</i> seeds in liquid nitrogen

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[1] Corresponding author