Abstract
An ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance and usefulness. As a conclusion we recommend the complementary inclusion of the EIM and MAC-ELISA substituting the HI for laboratories engaged in the diagnosis and surveillance of dengue.
ELISA Inhibition Method; MAC-ELISA; dengue
Serological diagnosis of dengue by an Elisa inhibition method (EIM)
R. J. Fernandez1
S. Vazquez1
Instituto de Medicina Tropical Pedro Kouri, Departamento de Virologia, Laboratório de Arbovírus, La Habana, Cuba
An ELISA Inhibition Method (EIM) was proposed for the serologic diagnosis of dengue, comparing its results with the Hemagglutination Inhibition (HI) and the IgM capture-ELISA (MAC-ELISA). Advantages and disadvantages of both methods are discussed according to sensitivity, specificity, performance and usefulness. As a conclusion we recommend the complementary inclusion of the EIM and MAC-ELISA substituting the HI for laboratories engaged in the diagnosis and surveillance of dengue.
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Publication Dates
-
Publication in this collection
04 June 2009 -
Date of issue
Sept 1990
