The authors describe results of isolations from rectal and throat swabs of paralitic polio-like cases in Brasilia. The specimens have been collected during a period of 13 months (January 64 to January 65) and sent by plane to the Instituto Oswaldo Cruz. From 47 samples examined, were isolated 28 strains of Poliovirus type 1, 4 of type 2, and two non-polio agents. One of the non-polio agents was pathogenic for baby-mice with typical lesions of Coxsackie A virus group. The age distribution suggests that children less than 4 years ol should be vaccinated as the first step in order to control polio infections in the studied area.