Micropropagation of lavender: a protocol for production of plantlets

Koefender, Jana; Manfio, Candida Elisa; Camera, Juliane N; Schoffel, André; Golle, Diego Pascoal

doi:10.1590/s0102-0536-20210409

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Research • Hortic. Bras. 39 (4) • Oct-Dec 2021 • https://doi.org/10.1590/s0102-0536-20210409 copy

Micropropagation of lavender: a protocol for production of plantlets

Micropropagação de lavanda: um protocolo para produção de mudas

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Lavender (Lavandula dentata) has several medicinal applications, both in the fresh form and after industrial processing. Productivity increase could be obtained through the mass propagation, expanding the possibility of obtaining raw material for production of essential oil. The objective of this work was to develop a micropropagation protocol from explants obtained from seeds. Aspects of in vitro germination, multiplication, elongation and rooting were evaluated and, in sequence, the acclimatization process as well. The in vitro germination of lavender seeds can be obtained using a medium composed of agar and water. For multiplication, the use of MS medium (Murashige & Skoog, 1962) added with 5.0 µM 6-benzylaminopurine to the nutrient medium is the most appropriate. For elongation and rooting, the use of growth regulators is unnecessary. Acclimatization can be obtained directly by transferring the explant to plastic bags with substrate, kept in a greenhouse. It was possible to develop a protocol for the in vitro cultivation (establishment, multiplication, elongation, rooting and acclimatization) of lavender from explants of seed origin.

Keywords:
Lavandula dentata; in vitro cultivation; essential oils; mass multiplication

Evaluated variables	NAA	BAP
Evaluated variables	NAA	0 µM	5.0 µM	10.0 µM	Means
Shoots per explant (n^o)	0.0 µM	2.00 a B	9.05 a A	4.70 a B	5.25
	1.0 µM	2.10 a A	3.95 b A	1.45 b B	2.50
	Means	2.05	6.50	3.07
	CV (%)	38
Shoot length (cm)	0 µM	2.29 a A	0.75 a B	1.31 a AB	1.45
	1.0 µM	1.30 a A	1.07 a A	1.04 a A	1.14
	Means	1.79	0.91	1.17
	CV (%)	26
Leaf (nº)	0 µM	13,5 a AB	10,1 b B	15,5 a A	13,03
	1.0 µM	11,7 a B	16,2 a A	13,9 a AB	13,93
	Means	12,6	13,15	14,7
	CV (%)	14
Hyperhidricity (%)	0 µM	1.0 a A	7.0 a B	9.0 a B	5.67
	1.0 µM	1.0 a A	6.0 b B	6.0 b B	4.33
	Means	1.0	6.5	7.5
	CV (%)	73
Callus formation (%)	0 µM	10 a A	90 a B	50.2 a AB	50.06
	1.0 µM	60 b A	60 a A	80 a A	66.6
	Means	35	75	65.1
	CV (%)	67

Evaluated variables	IAA	GA3
Evaluated variables	IAA	0 µM	5.0 µM	10.0 µM	Means
Shoots per explant (n^o)	0.0 µM	2.00 Aa	2.45 Aa	3.77 Aa	2.74
	0.5 µM	1.10 Ba	1.55 ABa	1.85 Aa	1.50
	1.0 µM	1.95 Aa	2.11 Aa	2.08 Aa	2.05
	Means	1.68	2.04	2.57
	CV (%)	26
Shoot length (cm)	0.0 µM	1.85 Bb	3.00 Aa	3.59 Aa	2.81
	0.5 µM	2.10 Ab	2.65 Aa	2.40 Aab	2.38
	1.0 µM	3.09 Aa	2.67 Aa	2.92 Ab	2.89
	Means	2.35	2.77	2.97
	CV (%)	29
Leaf (n^o)	0.0 µM	13.50 Aab	15.90 Aa	17.27 Aa	15.56
	0.5 µM	12.40 Ab	14.60 Aa	16.20 Aa	14.40
	1.0 µM	16.91 Aa	16.22 Aa	16.33 Aa	16.49
	Means	14.27	15.57	16.60
	CV (%)	24
Callus formation (%)	0.0 µM	10.00	0.50	0.73	3.74 A
	0.5 µM	0.30	0.40	0.60	0.43 A
	1.0 µM	0.55	0.22	0.67	0.48 A
	Means	3.62 A	0.37 A	0.66 A
	CV (%)	25
Hyperhidricity (%)	0.0 µM	10.00	0.20	0.27	3.49
	0.5 µM	0.10	0.20	0.20	0.17
	1.0 µM	0.00	0.22	0.33	0.19
	Means	3.37	0.21	0.27
	CV (%)	30
Root formation (%)	0.0 µM	20.00 Aa	0.30 Ba	0.18 Ba	6.83
	0.5 µM	0.40 Ab	0.30 Aa	0.30 Aa	0.33
	1.0 µM	0.09 Ab	0.33 Aa	0.33 Aa	0.25
	Means	6.83	0.31	0.27
	CV (%)	30

Treatments	Survival (%)	Plant height (cm)
Cultivation flask sealed with aluminum foil	46.15 b*	2.23 a
Cultivation flask sealed with plastic film	93.33 a	3.96 a
Cultivation flask without sealing	80.00 ab	3.45 a
Plastic bag for seedlings direct transplant	83.33 ab	3.20 a
CV (%)	15	36

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[TFN1] *Means followed by uppercase letter in the line and lowercase letter in the column are not different from each other by the Tukey test, p<0.05.