LEARNING BY IMAGES
Saphenous vein graft thrombus findings by scanning electron microscopy in a patient with acute myocardial infarction
Marcela Dias BorgesI; André Haraguti AguilleraI; José Joaquim BrilhanteII; Adriano CaixetaII
IEscola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil
IIHospital Israelita Albert Einstein, São Paulo, SP, Brazil
Corresponding author
An eighty-year-old male patient with a history of prior (19 years) coronary artery bypass graft surgery was admitted to the hospital with non ST-segment elevation myocardial infarction (NSTEMI). During the hospital stay he was taking acetylsalicylic acid 100mg per day, a loading dose of 600mg clopidogrel, and low molecular weight heparin 1mg/kg twice a day. Twenty-four hours later the patient underwent coronary angiography, which showed a 90% obstruction in the mid portion of the saphenous vein graft to obtuse marginal with signs of degeneration and local thrombus (Figure 1). Thrombus aspiration was performed with a 6-Fr ExportTM catheter (Medtronic, Santa Rosa, CA, USA), which removed small reddish colored fragments. They were fixed in 2,5% glutaraldehyde in a 0.1M sodium cacodilate buffer. The material was processed following the GOTO protocol in which the fragments were washed with osmium tetroxide and titanic acid, after which they were dried in a critical-point device and a golden bath. Scanning electron microscopy and high definition photos (3,000 to 27,221x magnification) were obtained by the FEI QuantaTM FEG SEM device (FEI Company, Hillsboro, OR, USA). The images showed that the thrombus was rich in activated platelets, with few erythrocytes or inflammatory cells. Many cholesterol crystals were observed (Figures 2 to 5). The fibrin networks were sparse and thin, which is compatible with a short ischemic time and recent thrombus formation.
The recent use of catheter aspiration in STEMI patients allows in vivo study of thrombus composition as well as its architectural and dynamic formation. Acute coronary thrombosis is caused by a rupture or erosion of an atherosclerotic plaque with subsequent aggregation of unstable platelets, fibrin networks with entrapped erythrocytes and inflammatory cells. The dynamic process of thrombus formation seems to be related to ischemic time, to the plaque's anatomical underlying substrate and to clinical variables(1). By scanning electron microscopy, Silvain et al. reported the composition of intracoronary thrombi showing that ischemic time in patients with STEMI had a positive correlation with fibrin content and a negative correlation with platelet content(2). In the present case, the first thus far to describe the composition of the thrombus aspirated from a degenerated saphenous vein graft by scanning electron microscopy, the findings of great amounts of small-dimension cholesterol crystals (<10µm) is remarkable, substantially differing from the thrombus composition in native coronary arteries(2,3) in which the finding of cholesterol crystals is rare. This finding may explain, at least in part, the higher prevalence and greater severity of distal embolization phenomena found in percutaneous intervention in saphenous vein grafts.
REFERENCES
- 1. Yunoki K, Naruko T, Sugioka K, Inaba M, Itoh A, Haze K, et al. Thrombus aspiration therapy and coronary thrombus components in patients with acute ST-elevation myocardial infarction. J Atheroscler Thromb. 2013;20(6):524-37.
- 2. Silvain J, Collet JP, Nagaswami C, Beygui F, Edmondson KE, Bellemain-Appaix A, et al. Composition of coronary thrombus in acute myocardial infarction. J Am Coll Cardiol. 2011;57(12):1359-67.
- 3. Yunoki K, Naruko T, Inoue T, Sugioka K, Inaba M, Iwasa Y, et al. Relationship of thrombus characteristics to the incidence of angiographically visible distal embolization in patients with ST-segment elevation myocardial infarction treated with thrombus aspiration. JACC Cardiovasc Interv. 2013;6(4):377-85.
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Publication Dates
-
Publication in this collection
15 Oct 2013 -
Date of issue
Sept 2013
