Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells

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Microphotometric scanning of chromatid gaps and breaks induced by AluI and BamHI in Chinese hamster ovary cells

Wilner Martínez-López ^I,II; Rossana Bonomi^I; Gustavo A. Folle^I; Máximo E. Drets^I

^IDivision of Human Cytogenetics and Quantitative Microscopy, Instituto de Investigaciones Biológicas Clemente Estable, Avda. Italia 3318,11600 Montevideo, Uruguay. Fax: [phone]. E-mail: [e-mail]. Send correspondence to W.M.-L.

^IIInstituto de Biología, Facultad de Ciencias, Tristán Narvaja 1674, 11200 Montevideo, Uruguay

ABSTRACT

Chromatid gaps and breaks induced by the restriction endonucleases AluI and BamHI in the long arm of chromosome 1 of Chinese hamster ovary cells were microphotometrically scanned and mapped to a quantitative G-band map. More than 50% of chromatid breaks appeared as chromatin losses of greater than 5% of the total arm length. The majority of chromatid gaps and breaks as well as chromatin losses induced by both restriction endonucleases were non-randomly located in a region from 0.35 to 0.65 relative length units of the long arm of chromosome 1. We suggest that the access of these endonucleases to chromosomal DNA depends on the local organization of the chromatin.

Keywords: microphotometric scanning; chromatid gaps; AluI; BamHI; Chinese hamster; ovary cells.

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Publication Dates

• Publication in this collection
  31 Oct 2006
• Date of issue
  1996