The effect of indigenous probiotics on egg hatchability and larval viability of Clarias gariepinus

O efeito dos probióticos naturais sobre a eclodibilidade de ovos e viabilidade das larvas de Clarias gariepinus

Abstracts

The effect of a mixture of four indigenous bacterial genera composed of Bacillus, Pseudomonas, Acinetobacter and Flavobacterium on egg hatchability and larval viability of Clarias gariepinus was investigated. The fertilized eggs were distributed into glass Petri dishes (100 mm diameter) containing 50 ml of water at graded level of mixed indigenous probiotics ranging from 0-10(8) cells/ml. The incubation time increased from 17 hours at 0 cfu/ml to 22 hours at 10(8) cfu/ml. The mean hatching rate increased from 8.70% at 0 cfu/ml to 53.85% at 10(8) cfu/ml. The highest larval survival of 71.43% recorded at 10(8) cfu/ml where the highest hatching rate was observed, was significantly higher than the larval survival rate observed at the other concentrations. All yolk sac larvae at 0 and 10¹ cfu/ml died before the end of yolk sac period. These results imply that the incubation time, hatching rate and larval survival of Clarias gariepinus increased with increase in bacterial load of water up to 10(8) cells/ml, the highest dose employed. Further investigations are needed to establish the optimal and threshold doses.

Clarias eggs; incubation; rearing; indigenous probiotics; yolk sac larvae

O efeito de uma mistura de quatro géneros bacterianos naturais compostos de Bacillus, Pseudomonas, Acinetobacter e Flavobacterium sobre a eclodibilidade dos ovos e viabilidade das larvas de Clarias gariepinus foi investigado. Os ovos fertilizados foram distribuídos em placas de Petri de vidro (100 mm de diâmetro) contendo 50 ml de água, adicionando-se probióticos naturais mistos que variaram de 0-10(8) células/ml. O tempo de incubação aumentou de 17 horas a 0 ufc/ml para 22 horas a 10(8) ufc/ml. A taxa de eclosão média aumentou de 8,70% a 0 ufc/ml para 53,85% a 10(8) ufc / ml. A maior taxa de sobrevivência das larvas foi de 71,43% observada em 10(8) ufc/ml, significativamente mais elevada do que a taxa de sobrevivência das larvas observada nas outras concentrações. Todas as larvas em fase embrionária na concentração de 0 e 10¹ cfu/ml morreram antes do final do período embrionário da gema. Estes resultados implicam que o tempo de incubação, a taxa de eclosão e a sobrevivência das larvas de Clarias gariepinus apresentaram os melhores resultados com a concentração bacteriana na água de até 10(8) células/ml, ou seja, com a dose mais elevada utilizada. Investigações adicionais são necessárias para estabelecer as doses ótimas e os níveis limites.

Ovos de Clarias; incubação; criação; probióticos naturais; larvas de gemas embrionárias

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Publication Dates

• Publication in this collection
  16 Sept 2014
• Date of issue
  Mar 2012