CHARACTERIZATION OF TYPICAL AND ATYPICAL MALASSEZIA SPP. FROM CATTLE AND DOG BY RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS

CARACTERIZAÇÃO DE ISOLADOS TÍPICOS E ATÍPICOS DEMALASSEZIA SPP. PROVENIENTES DE BOVINOS E CÃES UTILIZANDO A ANÁLISE DO DNA POLIMÓRFICO AMPLIFICADO ALEATORIAMENTE

ABSTRACT

There are few numbers of biochemical tests for specie classification in the genus Malassezia and these can to fail in the identification of the atypical isolates. In this study, typical and atypical isolates were analysed by random amplification of polymorphic DNA (RAPD) to compare with biochemical-physiological characteristics of the Malassezia species from bovine and canine ears. RAPD band patterns using OPA4 primer clustered all isolates according its biochemicalphysiological characteristics in the species from cattle and dog. Malassezia nana and M. sympodialis isolates were sub-clustered in separated sub-branches and both were from a different branch of the other species. The DNA pattern of the two atypical lipid-dependent M. pachydermatis strains was similar with of other typical strains but it did not show the one specific band of 200bp. Future studies in the specific RAPD bands of genetic profiles can be important to corroborate the identification of typical and atypical isolates of the genus Malassezia.

KEY WORDS
Malassezia spp.; identification; atypical isolates; RAPD analysis