ENTEROBACTERIA ISOLATION IN BROILER CARCASSES FROM COMMERCIAL ESTABLISHMENTS IN FORTALEZA, CEARÁ STATE, BRAZIL

Cardoso, W.M.; Oliveira, W.F. de; Romao, J.M.; Sampaio, F.A.C.; Moraes, T.G.V.; Teixeira, R.S.C.; Câmara, S.R.; Salles, R.P.R.; Siqueira, A.A. de; Nogueira, G.C.

doi:10.1590/1808-1657v73p3832006

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Scientific Article • Arq. Inst. Biol. 73 (4) • Oct-Dec 2006 • https://doi.org/10.1590/1808-1657v73p3832006 copy

ENTEROBACTERIA ISOLATION IN BROILER CARCASSES FROM COMMERCIAL ESTABLISHMENTS IN FORTALEZA, CEARÁ STATE, BRAZIL

ISOLAMENTO DE ENTEROBACTÉRIAS EM CARCAÇAS DE FRANGOS DE CORTE PROCEDENTES DE PONTOS COMERCIAIS DE FORTALEZA, ESTADO DO CEARÁ, BRASIL.

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Processing of poultry products requires a severe microbiological quality control, considering they are one of the main sources of foodborne infections. The objective of this research was to perform the isolation of enterobacteria in broiler carcasses from commercial establishments in the Metropolitan Region of Fortaleza in Ceará State, Brazil. Broiler carcasses were collected and selected as fresh (n = 14), refrigerated (n = 18) and frozen (n = 19). Carcasses were submitted to a rinsing method, followed by pre-enrichment and enrichment with Rappaport-Vassiliadis and Selenite-Cystine, streaked on plates with Brilliant Green, MacConkey and Salmonella-Shigella agars, and to a presumptive biochemical identification. It was verified that all broiler carcasses categories presented enterobacteria contamination, with the following frequency of isolation: Proteus sp., 66.7%; Enterobacter sp., 15.7%; Citrobacter sp., 2%; Escherichia coli, 47.1%; Klebsiella sp., 11.8%; Shigella sp., 5.9%, and Salmonella sp. 11.8%. It was observed that no combination of culture media was able to detect all enterobacteria contamination in the broiler carcasses. Thus, it may be necessary the use of several combinations of culture media to determine the real microbiological quality of broiler carcasses.

KEY WORDS
Enterobacteria; broiler carcass; culture media; microbiological quality; Salmonella ; methodology

Samples	Fresh		Refrigerated		Frozen		Total
	n=14		n=18		n=19		n=51
Bacteria		(%)		(%)		(%)		(%)
Proteus sp.	13^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(92.8)	11 ^b a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(61.1)	10 ^b a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(52.6)	34/51	(66.7)
Enterobacter sp.	4^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(28.6)	2 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(11.1)	2 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(10.5)	8/51	(15.7)
Citrobacter sp.	0 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(0)	0 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(0)	1 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(5.3)	1/51	(2)
Escherichia coli.	10^b a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(71.4)	9 ^{a b} a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(50)	5 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(26.3)	24/51	(47.1)
Klebsiella sp.	0 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(0)	3 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(16.7)	3 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(15.8)	6/51	(11.8)
Shigella sp.	2 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(14.3)	1 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(5.5)	0 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(0)	3/51	(5.9)
Salmonella sp.	2 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(14.3)	3 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(16.7)	1 ^a a, b – the different letters mean the existence of significant difference among the values (p < 0.05).	(5.3)	6/51	(11.8)

Bacteria	Culture media						Positive Carcass Samples
Bacteria	RB	SB	RM	SM	RS	SS
Total	9 a	16 a	41 bc	44 c	32 b	31 b	82
Proteus sp.	3^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	6^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	13^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^c a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	28^d a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	11^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	20^c a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	34
Enterobacter sp.	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	5^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	5^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	2 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	8
Citrobacter sp.	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	1 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	1 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	1 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	1
Escherichia coli	6^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	10^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	19^c a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	9^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	16^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^c a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	5^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	24
Klebsiella sp.	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	3 ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	2 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	3 ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	6
Shigella sp.	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	1^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	2 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	3 ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	3
Salmonella sp.	3 ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	3 ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	1 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	0 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	1 ^a a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05). ^b a,b,c,d The different letters mean the existence of significant difference among the values (p < 0,05).	6

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