In the present paper the author gives the final results of his studies about the significance of the two annex cells of the stomata of the Rubiaceae as anatomical family character, part of which has been previously published. After having verified that in the Order Rubiales the Family Rubiaceae is the unique to show stomata provided with two annex cells differing in size and shape, the author has extended his observation over 553 species belonging to 107 genera, having not found till the present time a single discrepant case. Even though in the future some species not yet investigated come to show stomata devoid of ajinex cells, even In this case the annex cells do not lose their importance at least as specific or generic character. However, the author is inclined to consider, on the light of-the facts in hand, the two annex cells of the Rubiaceae stomata as good anatomical family character.