This paper refers to sex differentiation in one day chicks of the Rhode Island Red Breed, which has been selected by the laying capacity in the flock pertaining to the Escola Superior de Agricultura "Luiz de Queiroz". The A., taking as a basis the light staining of the anterior edge of the wing, the fair ring of down of the chick leg, immediately a bove the tibiotarsic joint, and dark spot that occurs sometimes near the posterior angle of the eye, besides other characters of minor importance, was able to separate correctly 197 out of 201 chicks, what represents 98,01% of the total number.