TEST OF EXUDATE pH IN RICE SEEDS

SANTOS, MARIELY DE ABREU DOS; OLIVEIRA, IZABELA CRISTINA DE; NOGUEIRA, GRAZIELY ALVES; SILVA, JOSUÉ BISPO DA; CANDIDO, ANA CARINA DA SILVA; ALVES, CHARLINE ZARATIN

doi:10.1590/1983-21252019v32n412rc

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Agronomy • Rev. Caatinga 32 (4) • Oct-Dec 2019 • https://doi.org/10.1590/1983-21252019v32n412rc copy

TEST OF EXUDATE pH IN RICE SEEDS

TESTE DO pH DO EXSUDATO EM SEMENTES DE ARROZ

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The objective of this study was to evaluate the efficiency of a pH test of the exudate (with and without tegument) as an indicator of the physiological quality of rice seeds. The experimental design was completely randomized, with seven lots and four replications. The seven lots were assessed for water content and initial physiological quality by means of germination, first germination count, emergence, emergence speed index and tetrazolium tests. The pH test of the exudate was conducted with 100 seeds of each treatment, with tegument and without tegument. The seeds were individually soaked in 2.0 mL distilled water for different periods (20, 40, and 60 minutes) and three temperatures (20, 25, and 30 °C). The results were submitted to the Scott Knott test at 5% probability. The pH test of the exudate is a promising method to evaluate the physiological quality of rice seeds by detecting differences in vigor between marketable lots. This test should be conducted with rice seed without tegument, at 25 °C, with a soak time of 60 minutes.

Keywords:
Oryza sativa; Physiological quality; Vigor

LOTS	WC	FGC	G	SE	ESI	TZ
	(%)	(%)	(%)	(%)	-	(%)
1	10.20	67 a	92 a	89 a	4.43 a	89 a
2	10.03	73 a	90 a	83 b	3.15 b	92 a
3	10.86	59 b	80 b	76 c	2.28 c	78 b
4	10.15	71 a	85 b	83 b	3.14 b	88 a
5	10.89	57 b	81 b	80 b	3.08 b	78 b
6	10.06	57 b	80 b	68 c	2.18 c	73 c
7	10.19	60 b	83 b	72 c	2.29 c	78 b
F	-	7.41^* * Significant by F test at 5% probability. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.	8.46^* * Significant by F test at 5% probability. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.	16.69^* * Significant by F test at 5% probability. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.	31.88^* * Significant by F test at 5% probability. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.	21.94^* * Significant by F test at 5% probability. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.
CV%	-	7.83	4.19	4.25	9.52	3.75

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E-mail: caatinga@ufersa.edu.br

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[1] *Corresponding author

	20 ºC
Lot	20 min	40 min	60 min
1	98	94	93
2	98	94	93
3	96	94	91
4	98	97	94
5	96	96	91
6	96	94	91
7	97	94	93
F	0.50^ns ns not significant. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.	0.36^ns ns not significant. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.	0.55^ns ns not significant. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.
CV%	3.69	3.64	3.24
	25 ºC
Lot	20 min	40 min	60 min
1	98 a	96	93
2	92 a	93	93
3	95 b	95	92
4	98 a	96	94
5	94 b	93	93
6	92 b	91	90
7	93 b	92	90
F	2.90^* * Significant by F test at 5% probability.	0.83^ns ns not significant. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.	2.40^ns ns not significant. CV - coefficient of variation. Means follow by the same letter in the column do not differ between 5% probability by the Scott-Knott test.
CV%	2.99	3.08	2.10
	30 ºC
Lot	20 min	40 min	60 min
1	98 a	96 a	92 a
2	96 b	93 a	90 a
3	89 b	87 b	86 b
4	99 a	98 a	97 a
5	97 a	93 a	92 a
6	89 b	84 b	82 b
7	91 b	86 b	84 b
F	4.90^* * Significant by F test at 5% probability.	3.83^* * Significant by F test at 5% probability.	3.42^* * Significant by F test at 5% probability.
CV%	4.20	6.12	6.23

	20 ºC
Lot		20 min	40 min	60 min
1	94 a		92 a		92 a
2	95 a		89 a		88 a
3	85 a		79 b		78 b
4	90 a		88 a		86 a
5	89 a		83 b		81 b
6	66 c		63 c		61 c
7	78 b		77 b		77 b
F	12.14^* * Significant by F test at 5% probability.		15.81^* * Significant by F test at 5% probability.		15.62^* * Significant by F test at 5% probability.
CV%	6.90		6.06		6.41
	25 ºC
Lot		20 min	40 min	60 min
1	95 a		94 a		90 a
2	91 a		86 a		76 b
3	74 c		62 c		64 c
4	84 b		79 b		77 b
5	82 b		77 b		73 b
6	76 c		62 c		60 c
7	75 c		69 c		68 c
F	15.73^* * Significant by F test at 5% probability.		15.72^* * Significant by F test at 5% probability.		10.23^* * Significant by F test at 5% probability.
CV%	5.00		8.02		0.86
Lot	30 ºC
Lot	20 min		40 min		60 min
1	92 a		86 a		84 a
2	91 a		83 a		82 a
3	82 b		76 a		73 b
4	85 b		77 a		76 b
5	85 b		80 a		74 b
6	76 c		61 b		63 b
7	82 b		77 a		70 b
F	6.46^* * Significant by F test at 5% probability.		7.14^* * Significant by F test at 5% probability.		4.06^* * Significant by F test at 5% probability.
CV%	5.13		7.77		9.47