A method was developed and validated in-house for determination of ochratoxin A (OTA) in wine using charge coupled device (CCD) to acquire the fluorescence images of mycotoxin from high-performance thin-layer chromatography (HPTLC) under UV lamp. The method showed a mean recovery of 90.4%. The quantification and detection limist were 0.1 µg L-1 and 0.016 µg L-1 per spot, respectively. The results of validation confirmed the efficiency of the method, which is sensitive enough to be used to quantify OTA in wine. The occurrence of OTA in Brazilian wines was evaluated. OTA was found in one sample at a level of 4.5 µg L-1, which is higher than the limit of 2 µg L-1 considered acceptable by the Scientific Commission of the European Communities. This study demonstrated the applicability of HPTLC using CCD as a tool to determine OTA in wine.
ochratoxin A; wine; charge-coupled device imaging; high-performance thin-layer chromatography
