Oleic acid acutely impairs glucose homeostasis in standard chow diet but not high-fructose, high-fat diet-fed mice by acting on free fatty acid receptor 1

Mansour, Rehab; El-Fayoumi, Hassan Mahmoud; Fahmy, Ahmed; Ibrahim, Islam Ahmed Abd El-Hamed

doi:10.1590/s2175-97902022e20710s

Rehab Mansour

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Egypt,

Hassan Mahmoud El-Fayoumi

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Egypt,

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kantara Sinai University, Egypt

Ahmed Fahmy

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Egypt,

Islam Ahmed Abd El-Hamed Ibrahim ^**Correspondence: I. A. A. El-H. Ibrahim. Department of Pharmacology and Toxicology. Faculty of Pharmacy. Zagazig University, 44519 Egypt. Phone/Fax: +20552303266. E-mail: eaebrahiem@pharmacy.zu.edu.eg.

Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Egypt,

http://orcid.org/0000-0002-8931-465X

*Correspondence: I. A. A. El-H. Ibrahim. Department of Pharmacology and Toxicology. Faculty of Pharmacy. Zagazig University, 44519 Egypt. Phone/Fax: +20552303266. E-mail: eaebrahiem@pharmacy.zu.edu.eg.

Figures (3)
Tables (2)

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FIGURE 1
Effect of feeding a high-fructose, high-fat diet (HFrHFD) for 16 weeks on the body weight, intraperitoneal glucose tolerance test (IPGTT), insulin resistance, and levels of hepatic phosphatidylinositol 4,5 bisphosphate (PIP2) and hepatic diacylglycerol (DAG). Graphical presentation of A: body weights; B: IPGTT; C: area under the curve (AUC) of the IPGTT; D: serum insulin level; E: homeostasis model of insulin resistance (HOMA-IR) index; F: hepatic levels of PIP2; and G: hepatic levels of DAG. Statistical analysis was performed using an unpaired Student’s t-test; values are represented as mean ± standard error of the mean (S.E.M.). n = 6 in the IPGTT; 5 for the serum insulin and HOMA-IR; 3 for the PIP2 and DAG. **, *** P < 0.01, 0.001.

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FIGURE 2
Acute effect of OA on intraperitoneal glucose tolerance test (IPGTT), and levels of hepatic phosphatidylinositol 4,5 bisphosphate (PIP2), and hepatic diacylglycerol (DAG) in the standard chow diet (SCD)-fed mice. Graphical presentation of A: IPGTT; B: AUC of the IPGTT; C: hepatic levels of PIP2; and D: hepatic levels of DAG. SCD: Mice were fed a standard show diet for 16 weeks. SCD + OA: SCD-fed mice received OA (40 mg/kg/i.p.). SCD + OA + GW: SCD-fed mice received GW1100 (0.4 mg/kg/i.p), 30 min before OA administration. OA and GW1100 were dissolved in the same vehicle (25% DMSO, 75% distilled water; 100 μl/40 g body weight). Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc test for the IPGTT and one-way ANOVA followed by Tukey’s post hoc test for other parameters; values are represented as mean ± S.E.M. n = 6 in the IPGTT; 3 for PIP2 and DAG. In IPGTT, ### P < 0.001 vs. SCD, &&, &&& P < 0.01 and 0.001, respectively, vs. SCD + OA + GW group. * P < 0.05.

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FIGURE 3
Acute effect of OA on IPGTT, hepatic PIP2, and hepatic DAG in the HFrHFD-fed mice. Graphical presentation of A: IPGTT; B: AUC of the IPGTT; C: hepatic levels of PIP2; and D: hepatic levels of DAG. HFrHFD: Mice were fed a high-fructose, high-fat diet for 16 weeks. HFrHFD + OA: HFrHFD-fed mice received OA (40 mg/kg/i.p.). HFrHFD + OA + GW: HFrHFD-fed mice received GW1100 (0.4 mg/kg/i.p), 30 min before OA administration. OA and GW1100 were dissolved in the same vehicle (25% DMSO, 75% distilled water; 100 μl/40 g body weight). Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc test for the IPGTT and one-way ANOVA followed by Tukey’s post hoc test for other parameters; values are represented as mean ± S.E.M. n = 6 in the IPGTT; 3 for PIP2 and DAG. In IPGTT, && P < 0.01 vs. HFrHFD + OA + GW group, ### P < 0.001 vs. HFrHFD group. **, *** P < 0.01 and 0.001, respectively.

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TABLE I
Composition of standard chow diet (SCD) and high-fructose, high-fat diet (HFrHFD)

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TABLE II
Fatty acid composition of standard chow diet (SCD) and high-fructose, high-fat diet (HFrHFD)

	SCD	HFrHFD
Carbohydrate	60%	46%
Protein	22%	18%
Fat	2.5%	24%
Others (fibers, minerals, etc.)	15.5%	12%

	Fatty acids	Relative concentration (%)
	Fatty acids	HFrHFD	SCD
1	Capric acid (C10:0)	0.12	---
2	Lauric acid (C12:0)	0.13	---
3	Tridecanoic acid (C13:0)	0.15	---
4	Myristic acid (C14:0)	2.65	---
5	Myrist-oleic acid (14.1)	0.27	---
6	Pentadecanoic acid (C15:0)	0.38	---
7	Cis-10-Pentadecenoic (C15:1)	0.45	---
8	Palmitic acid (C16:0)	21.91	10.71
9	Palmitoleic acid (C16:1n 7)	1.05	1.53
10	Palmitoleic acid (C16:1n 9)	0.59	----
11	Heptadecanoic acid (C17:0)	0.95	0.29
12	Stearic acid (C18:0)	29.61	2.17
13	Oleic acid (C18:1n9c)	34.23	26.15
14	Elaidic acid (C18:1n9t)	3.01	0.87
15	Linoleic acid (C18:2n6c)	2.15	45.29
16	Linolelaidic acid (C18:2n6t)	0.39	3.75
17	Arachidic acid (C20:0)	0.30	6.67
18	γ-Linolenic acid (C18:3n6)	0.13	1.84
19	Cis-11- Eicosenoic acid (C20.1)	0.34	---
20	Linolenic acid (C18:3n3)	0.39	---
21	Cis -11,14- Eicosadienoic acid (C 20.2)	0.20	0.26
22	Behenic Linolenic acid (C22:0)	0.37	0.48

[1] *Correspondence: I. A. A. El-H. Ibrahim. Department of Pharmacology and Toxicology. Faculty of Pharmacy. Zagazig University, 44519 Egypt. Phone/Fax: +20552303266. E-mail: eaebrahiem@pharmacy.zu.edu.eg.

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Oleic acid acutely impairs glucose homeostasis in standard chow diet but not high-fructose, high-fat diet-fed mice by acting on free fatty acid receptor 1

Abstract