Efeitos de diferentes extratos do cogumelo Agaricus blazei Murill sobre
                  o perfil hematológico de camundongos com tumor de Ehrlich

Verçosa Júnior, D.; Ferraz, V.P.; Duarte, E.R.; Oliveira, N.J. F.; Soto-Blanco, B.; Cassali, G.D.; Melo, M.M.

doi:10.1590/1678-4162-7595

Acessibilidade / Reportar erro

Brasil

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Español English

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Veterinary Medicine • Arq. Bras. Med. Vet. Zootec. 67 (3) • May-Jun 2015 • https://doi.org/10.1590/1678-4162-7595 copiar

Efeitos de diferentes extratos do cogumelo Agaricus blazei Murill sobre o perfil hematológico de camundongos com tumor de Ehrlich

Autoria SCIMAGO INSTITUTIONS RANKINGS

O objetivo deste estudo foi investigar o efeito de diferentes extratos do cogumelo Agaricus blazeii Murril (ABM) sobre o perfil hematológico de camundongos Swiss portadores de tumor de Ehrlich sólido. Três frações (extrato total, polissacarídeos e sobrenadante) dos extratos de ABM foram obtidas por quatro métodos (sonificador, banho-maria, em pH 4 ou pH 7) e administradas para camundongos durante 21 dias. Soluções de polissacarídeos foram analisadas por cromatografia gasosa e líquida, que mostraram concentrações de glucose e manose. O método de extração influenciou o grau de polimerização da glicose e a relação manose/glucose. O tratamento com o sobrenadante de ABM (em pH 7 e banho-maria) estava associado com reduzidas concentrações de leucócitos e linfócitos, além de alterar a porcentagem de linfócitos CD4+ e CD8+ em camundongos portadores de tumor sólido de Ehrlich. O tratamento com extratos de ABM, obtidos tanto em banho-maria como no sonificador em pH 4, resultou nas mais baixas contagens de linfócitos, independentemente da presença do tumor, e nos maiores valores de granulócitos em camundongos com tumor de Ehrlich. Conclui-se que os diferentes métodos de extração com as respectivas frações de A. blazei são capazes de intereferir no perfil hematológico de camundongos com tumor sólido de Ehrlich.

camundongo; tumor sólido de Ehrlich; polissacarídeos; perfil hematológico

Treatments for four experiments with two tumor groups with four treatments
Experiment 1: GI – without tumor
A	Distilled water
B	Total solution at pH 7 extracted in a water bath
C	Polysaccharide solution at pH 7 extracted in a water bath
D	Supernatant solution at pH 7 extracted in a water bath
Experiment 1: GII – with Ehrlich tumor
A	Distilled water
B	Total solution at pH 7 extracted in a water bath
C	Polysaccharide solution at pH 7 extracted in a water bath
D	Supernatant solution at pH 7 extracted in a water bath

Experiment 2: GI – without tumor
E	Distilled water
F	Total solution at pH 4 extracted in a water bath
G	Polysaccharide solution at pH 4 extracted in a water bath
H	Supernatant solution at pH 4 extracted in a water bath
Experiment 2: GII – with Ehrlich tumor
E	Distilled water
F	Total solution at pH 4 extracted in a water bath
G	Polysaccharide solution at pH 4 extracted in a water bath
H	Supernatant solution at pH 4 extracted in a water bath

Experiment 3: GI – without tumor
I	Distilled water
J	Total solution at pH 7 extracted in an ultrasonic bath
K	Polysaccharide solution at pH 7 extracted in an ultrasonic bath
L	Supernatant solution at pH 7 extracted in an ultrasonic bath
Experiment 3: GII – with Ehrlich tumor
I	Distilled water
J	Total solution at pH 7 extracted in an ultrasonic bath
K	Polysaccharide solution at pH 7 extracted in an ultrasonic bath
L	Supernatant solution at pH 7 extracted in an ultrasonic bath

Experiment 4: GI – without tumor
M	Distilled water
N	Total solution at pH 4 extracted in an ultrasonic bath
O	Polysaccharide solution at pH 4 extracted in an ultrasonic bath
P	Supernatant solution at pH extracted in an ultrasonic bath
Experiment 4: GII – with Ehrlich tumor
M	Distilled water
N	Total solution at pH 4 extracted in an ultrasonic bath
O	Polysaccharide solution at pH 4 extracted in an ultrasonic bath
P	Supernatant solution at pH 4 extracted in an ultrasonic bath

Extraction method		Polymerizationdegree
water bath at pH 7	3.151	18
water bath at pH 4	2.348	13
ultrasonic bath at pH 7	1.864	10
ultrasonic bath at pH 4	1.763	10

Treatments			CD4+ lymphocytes %	CD8+ lymphocytes %
Experiment 1: pH 7 water bath extract
GI-A		Distilled water	31.26	10.83
GII-A		Distilled water	31.62	18.12
GII-B		Total solution	32.11	20.24
GII-C		Polysaccharide solution	35.07	24.02
GII-D		Supernatant solution	37.35	18.40

Experiment 2: pH 4 water bath extract
GI-E		Distilled water	31.26	10.80
GII-E		Distilled water	31.62	18.00
GII-F		Total solution	26.79	22.21
GII-G		Polysaccharide solution	27.81	22.08
GII-H		Supernatant solution	29.17	20.31

Experiment 3: pH 7 ultrasonic bath extract
GI-I		Distilled water	31.26	10.83
GII-I		Distilled water	31.62	18.30
GII-J		Total solution	31.73	24.45
GII-K		Polysaccharide solution	28.32	16.94
GII-L		Supernatant solution	31.24	17.99

Experiment 4: pH 4 ultrasonic bath extract
GI-M	Distilled water		31.26	10.87
GII-M	Distilled water		31.62	18.27
GII-N	Total solution		34.05	19.68
GII-O	Polysaccharide solution		30.96	17.76
GII-P	Supernatant solution		28.81	24.37

Universidade Federal de Minas Gerais, Escola de Veterinária Caixa Postal 567, 30123-970 Belo Horizonte MG - Brazil, Tel.: (55 31) 3409-2041, Tel.: (55 31) 3409-2042 - Belo Horizonte - MG - Brazil
E-mail: abmvz.artigo@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

[1] E-mail: mariliamm@ufmg.br