POLYAMINES AS SALINITY BIOCHEMICAL MARKER IN CALLUS OF <i>Eucalyptus urograndis</i>

Lima, Giuseppina Pace Pereira; Piza, Isabela M. Toledo; Henrique, Andréa; Takaki, Massanori

doi:10.5902/198050981722

ABSTRACT

Biochemical markers have been used for the analysis of plant cells submitted to several types of stress, among them salinity. This work aimed at analyzing the effect of saline stress in callus of Eucalyptus urograndis on polyamine contents. Explants (hypocotyls) obtained from seeds were inoculated in callus inductive medium, submitted to different levels of NaCl and analyzed at 10, 20 and 30 days after the inoculation. The free polyamines were extracted, isolated and quantified using TLC (Thin-Layer Chromatography). Putrescine content was higher and a fall in the spermidine content was observed in callus submitted to salinity condition. The results showed that polyamine accumulation is related to NaCl exposure in callus of Eucalyptus urograndis. The decrease in spermine content could be used as a biochemical marker for Eucalyptus callus subjected to salinity.

Key words:
micropropagation; salt stress; polyamines; Eucalyptus.

RESUMO

Marcadores bioquímicos têm sido usados na análise de plantas submetidas a vários tipos de estresse, entre eles a salinidade. Este trabalho teve como objetivo analisar o estresse salino em calos de Eucalyptus urograndis em relação ao conteúdo de poliaminas. Explantes (hipocótilos) obtidos de sementes foram inoculados em meio indutivo de calos e foram submetidos a diferentes níveis de NaCl e analisados aos 10, 20 e 30 dias de inoculação. As poliaminas livres foram extraídas, isoladas e quantificadas usando TLC (Cromatografia de Camada Delgada). O conteúdo de putrescina foi maior, enquanto que o conteúdo de espermidina apresentou decréscimo, em calos submetidos a condições salinas. Os resultados mostram que o acúmulo de putrescina está relacionado com a exposição a NaCl em calos de Eucalyptus urograndis. A diminuição do conteúdo de espermina pode ser usada como marcador bioquímico de calos de Eucalyptus sujeitos à salinidade.

Palavras-chave:
micropropagação; estresse salino; poliaminas; Eucalyptus

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BIBLIOGRAPHY REFERENCES

ALI, R.M. Role of putrescine in salt tolerance of Atropa belladonna plant. Plant Science, v. 152, p. 173-179, 2000.
AZIZ, A.; MARTIN-TANGUY, J.; LARHER, F. Stress-induced changes in polyamine and tyramine levels can regulate proline accumulation in tomato leaf discs treated with sodium chloride. Physiologia Plantarum, v. 104, p. 195-202, 1998.
AZIZ, A.; MARTIN-TANGUY, J.; LARHER, F. Salt stress-induced proline accumulation and changes in tyramine and polyamines levels are linked to ionic adjustment in tomato leaf discs. Plant Science , v. 145, p. 83-91, 1999.
BORREL, A. et al. Polyamines inhibit lipid peroxidation in senescing oat leaves. Physiologia Plantarum , v. 99, p. 385-390, 1999.
BOUCHEREAU, A. et al. Polyamines and environmental challenges: recent development. Plant Science , v. 140, p. 103-125, 1999.
BRAY, E.A.; BAILEY-SERRES, J.; WERETILNYK, E. Responses to abiotic stress. In: BUCHANAN, B.; GRUISSEM, W.; JONES, R. (Eds.) Biochemistry & molecular biology of plants. Rockville, Maryland: American Society of Plant Physiologists, 2000. p. 1158-1203.
DI TOMASO, J.M.; SHAFF, J.E.; KOCHIAN, L.V. Putrescine-induced wounding and its effects on membrane integrity and ion transport processes in root corn seedlings. Plant Physiology, v. 90, p. 988-995, 1989.
FLORES, H.E.; GALSTON, A.W. Polyamines and plant stress. Activation of putrescine biosynthesis by osmotic shock. Science, v. 217, p. 1259-1261, 1982.
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SHEVYAOKOVA, N.I. et al. Metabolism of polyamines in NaCl-resistant cell lines from Nicotiana sylvestrys Plant Growth Regulation, v. 3, p. 365-369, 1985.
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Publication Dates

Publication in this collection
Jan-Jun 2003

History

Received
04 July 2002
Accepted
18 Nov 2002

This is an open-access article distributed under the terms of the Creative Commons Attribution License

[1] ALI, R.M. Role of putrescine in salt tolerance of Atropa belladonna plant. Plant Science, v. 152, p. 173-179, 2000.

[2] AZIZ, A.; MARTIN-TANGUY, J.; LARHER, F. Stress-induced changes in polyamine and tyramine levels can regulate proline accumulation in tomato leaf discs treated with sodium chloride. Physiologia Plantarum, v. 104, p. 195-202, 1998.

[3] AZIZ, A.; MARTIN-TANGUY, J.; LARHER, F. Salt stress-induced proline accumulation and changes in tyramine and polyamines levels are linked to ionic adjustment in tomato leaf discs. Plant Science , v. 145, p. 83-91, 1999.

[4] BORREL, A. et al. Polyamines inhibit lipid peroxidation in senescing oat leaves. Physiologia Plantarum , v. 99, p. 385-390, 1999.

[5] BOUCHEREAU, A. et al. Polyamines and environmental challenges: recent development. Plant Science , v. 140, p. 103-125, 1999.

[6] BRAY, E.A.; BAILEY-SERRES, J.; WERETILNYK, E. Responses to abiotic stress. In: BUCHANAN, B.; GRUISSEM, W.; JONES, R. (Eds.) Biochemistry & molecular biology of plants. Rockville, Maryland: American Society of Plant Physiologists, 2000. p. 1158-1203.

[7] DI TOMASO, J.M.; SHAFF, J.E.; KOCHIAN, L.V. Putrescine-induced wounding and its effects on membrane integrity and ion transport processes in root corn seedlings. Plant Physiology, v. 90, p. 988-995, 1989.

[8] FLORES, H.E.; GALSTON, A.W. Polyamines and plant stress. Activation of putrescine biosynthesis by osmotic shock. Science, v. 217, p. 1259-1261, 1982.

[9] GALSTON, A.W.; KAUR-SAWHNEY, R. Polyamines in plant physiology. Plant Physiology , v. 94, p. 206-210, 1990.

[10] GANGOPADHYAY, G. et al. Effects of salt and osmotic shocks on unadapted and adapted callus lines of tobacco. Plant Cell Tissue and Organ Culture, v. 49, p. 42-52, 1997.

[11] GONÇALVES, A.N. Reversion to juvenility and cloning of Eucalyptus urophylla S.T. Blake. In: SIMPÓSIO E IUFRO EM MELHORAMENTO GENÉTICO E PRODUTIVIDADE DE FLORESTAS DE RÁPIDO CRESCIMENTO, 1980, Águas de São Pedro. Anais... Águas de São Pedro, SP, 1980. p. 25-30.

[12] KRISHNAMURTHY, R. Amelioration of salinity effect in salt tolerance rice (Oryza sativa L.) by foliar application of putrescine. Plant Cell Physiology, v. 32, p. 699-703, 1991.

[13] LEFÈVRE, I.; GRATIA, E.; LUTTS, S. Discrimination between the ionic and osmotic components of salt stress in relation to free polyamine level in rice (Oryza sativa). Plant Science , v. 161, p. 943-952, 2001.

[14] LI, Z.-Y.; CHEN, S.Y. Isolation and characterization of a salt-and drought-inducible gene for S- adenosylmethionine decarboxylase from wheat (Triticum aestivum L.) Journal of Plant Physiology , v. 156, p. 386-393, 2000.

[15] MURASHIGE, T.; SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum , v. 15, p. 473-493, 1962.

[16] NIKNAM, S.R.; MCCOMB, J. Salt tolerance screening of selected Australian woody species - a review. Forestry Ecology Manager, v. 139, p. 1-19, 2000.

[17] RIEDELL, W.E. Effect of Ca²⁺ and polyamines on Na⁺ and Rb⁺ influx in excised maize roots. Physiologia Plantarum , v. 69, p. 299-304, 1987.

[18] REGGIANI, R. et al. Effect of K ions on polyamine levels in wheat seedling under anoxia. Journal of Plant Physiology , v. 142, p. 94-98, 1993.

[19] SHEVYAOKOVA, N.I. et al. Metabolism of polyamines in NaCl-resistant cell lines from Nicotiana sylvestrys Plant Growth Regulation, v. 3, p. 365-369, 1985.

[20] SUN, D.; DICKINSON, G.R. Responses to salt stress of 16 Eucalyptus species, Grevillea robusta, Lophostemon confertus and Pinus caribea var. hondurensis. Forestry Ecology Manager , v. 60, p. 1-14, 1993.

[21] VELIKOVA, V.; YORDANOV, I.; EDREVA, A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science , v. 151, p. 59-66, 2000.

[22] WILLANDINO, L. et al. Polyamine and free amino acid variations in NaCl-treated embryogenic maize callus from sensitive and tolerant cultivars. Revista Brasileira de Fisiologia Vegetal, v. 147, p. 179-185, 1996.

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