A fatal case of urosepsis due to Corynebacterium riegelii

MEDICAL

SHORT COMMUNICATION

A fatal case of urosepsis due to Corynebacterium riegelii

Gokhan Aygun^I, Kenan Midilli^I; Hatice Cilingir^I; Mesut Yilmaz^II; Aysegul Kutukcu^III; Engin Eker^III

^IIstanbul University , Cerrahpasa Medical Faculty, Microbiology and Clinical Microbiology Department, Istanbul, Turkey

^IIIstanbul Medipol University , Infectious Diseases and Clinical Microbiology Department, Istanbul, Turkey

^IIIIstanbul University , Cerrahpasa Medical Faculty, Psychiatry Department, Istanbul, Turkey

^{Send correspondence to}

ABSTRACT

Corynebacterium species other than Corynebacterium diphtheriae rarely cause infections in human but rather reside in flora, however they have been reported to cause opportunistic infections in both immunocompromised and immunecompetent patients. Here we report for the first time a case of an elderly female patient presenting with a fatal urosepsis caused by a recently defined pathogen, Corynebacterium riegelii, identified on second day after patient hospitalization leading to a progressive worsening and death of the patient on 6th day.

Key words: Corynebacterium riegelii, urosepsis, PCR amplification.

Corynebacterium riegelii, a recently defined Corynebacterium species, was first isolated and identified by Funke et al. (1997, 1998) from females with urinary tract infections, however fatal infections have never been reported (Winn et al., 2006).

A 79 years-old female was admitted to the psychiatry ward with difficulty in cooperation, loss of orientation and delirium and was put on antipsychotic and antihypertensive drugs. Physical exam was not remarkable. Laboratory findings showed white blood cell (WBC) count: 15.100/mm³ (80% neutrophils, 20% lymphocytes), hemoglobin: 12.7 g/dL, Blood Urea Nitrogen: 126 mg/dL, creatinine: 1.4 mg/dL, C-Reactive protein: 25 mg/dL (normal < 5 mg/dL) and a normal platelet count of 166.000 platelets/ mm³. Urinalysis showed pyuria with a WBC of 40-45 cells per high-power field (HPF). As she developed fever and signs of urinary tract infection on the second day of hospitalization, she was diagnosed with pyelonephritis and urosepsis. Following 2 sets of blood and urine cultures, she was started ciprofloxacin (200 mg iv every 12 hours) empirically. She developed tachypnea, respiratory distress, progressive increase in blood urea nitrogen and serum creatinine and died on 6^th day of hospitalization. Blood cultures remained negative, however, urine cultures obtained on second and third days of admission revealed > 10⁵ cfu/mL diphtheroid bacilli in pure culture. Colonies were green colored, 2-3 mm in diameter and opaque on Cystein-Lactose-Electrolyte-Deficient (CLED) agar (Becton Dickinson, USA) and non-hemolytic on sheep blood agar, non-motile, non-sporulated Gram positive bacilli. The methods used for determination of the biochemical profiles have been described previously (Funke et al., 1998). API Coryne strips revealed a profile number of 2001224 at 48 hours, and API 50CH reactions were performed with the 50 CHE medium (bioMérieux, Marcy l'Etoile, France). The isolate was catalase (+), esculin (-), urease (+), CAMP (-) and was preliminarily identified as C. riegelii.

The MICs were determined by E-test (AB Biodisk Sonla, Sweden) on Mueller Hinton agar at 35 ºC in ambient air for 20 h (Martinez-Martinez et al., 1995). The results were as follows: vancomycin: 0.25 mg/L, co-trimoxazole 2 mg/L, penicillin-G: 0.125 mg/L, ciprofloxacin: 0.064 mg/L, nalidixic acid: 16 mg/L, amikacin: 0.25 mg/L.

The identification of the isolate was also confirmed by 16S rRNA gene sequencing. For this purpose, bacterial DNA was obtained from overnight culture using a commercial DNA purification kit (High Pure PCR Template Preparation kit, Roche Diagnostics, Mannheim, Germany). Primers described previously were used during the PCR amplification and sequencing (Jonasson et al., 2002; Weisburg et al., 1991). The sequence obtained was compared with the sequences deposited at the GenBank using BLASTN 2.2.18+ program (Zhang et al., 2000). The sequenced segment of 1457 bp had 100% homology with the reference strain deposited in GenBank under the accession number EU848548.

Strong urease activity(i.e., positive within 5min in Christensen's urea broth) is one of the typical features of Corynebacterium riegelii. It forms white, non-lipophylic, non-hemolytic colonies on blood agar. Slow acid production from maltose but not from sucrose and glucose is a distinctive feature. API Coryne has been successfully used for C. riegelii identification. Bernard et al. (2002) reported four C. riegelii isolates: two from blood culture which were reported for the first time, one from urine culture and one from cord blood. He reported that none of the isolates produced propionic acid but the two strains tested for O-129 were found to be sensitive. Funke et al. (1998) determined MICs of various antimicrobial substances and found that C. riegelii strains were susceptible to cephalothin, chloramphenicol, ciprofloxacin, fusidic acid, gentamicin, penicillin, rifampin, tetracycline, and vancomycin but were resistant to cefetamet, ceftibuten, and fosfomycin.

Various identification systems have been used recently with great success including API Coryne. Amplification of 16S rRNA genes and sequencing is another tool used for identification (Tang et al., 2000). More recently, Van den Velde et al. (2007) have suggested that species of corynebacteria would be more correctly identified based on their cellular fatty acid profiles (ie, for the C14 to C20 fatty acids).

As there was no other comorbidity to cause progressive clinical worsening and no other pathogen isolated, the patient's death was attributed to urosepsis due to C. riegelii. The microorganism was possibly acquired prior to admittance and only manifested afterwards. There were no documented prior infections with C. riegelii in any other patient in the hospital.

Although ciprofloxacin was started empirically and continued based on susceptibility results, there is no clinical data in the literature regarding clinical efficacy of ciprofloxacin to infections with C. riegelii.

Coryneform bacteria from urine samples, even when growing in pure cultures, are usually considered contaminants by many clinical laboratories. It should be kept in mind that C. riegelii may cause fatal urinary tract infections. We therefore emphasize the importance of identifying coryneform bacteria to the species level whenever they are recovered in pure culture from clinical specimens. More data is required regarding clinical features and treatment strategies on infections with recently defined Corynebacterium species.

Submitted: August 29, 2011

Approved: September 10, 2012

All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.

• Bernard KA, Munro C, Wiebe D, Ongsansoy E (2002) Characteristics of rare or recently described Corynebacterium species recovered from human clinical material in Canada. J Clin Microbiol 40:4375-4381.
• Funke G, Lawson PA, Collins MD (1998) Corynebacterium riegelii sp. nov., an unusual species isolated from female patients with urinary tract infections. J Clin Microbiol 36:624-627.
• Funke G, von Graevenitz A, Clarridge JE, 3rd Bernard KA (1997) Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10:125-159.
• Jonasson J, Olofsson M, Monstein HJ (2002) Classification, identification and subtyping of bacteria based on pyrosequencing and signature matching of 16S rDNA fragments. APMIS 110:263-272.
• Martinez-Martinez L, Ortega MC, Suarez AI (1995) Comparison of E-test with broth microdilution and disk diffusion for susceptibility testing of coryneform bacteria. J Clin Microbiol 33:1318-1321.
• Tang YW, Von Graevenitz A, Waddington MG, Hopkins MK, Smith DH, Li H, Kolbert CP, Montgomery SO, Persing DH (2000) Identification of coryneform bacterial isolates by ribosomal DNA sequence analysis. J Clin Microbiol 38:1676-1678.
• Van den Velde S, Lagrou K, Desmet K, Wauters G, Verhaegen J (2006) Species identification of corynebacteria by cellular fatty acid analysis. Diagn Microbiol Infect Dis 54:99-104.
• Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697-703.
• Winn W, Allen S, Janda W, Koneman E, Procop G, Schreckenberger P, Woods G (2006) Koneman's Color Atlas and Textbook of Diagnostic Microbiology. Philadelphia, Lippincott Williams & Wilkins: 783-857.
• Zhang Z, Schwartz S, Wagner L, Miller W (2000) A greedy algorithm for aligning DNA sequences. J Comput Biol 7:203-214.

Publication Dates

• Publication in this collection
  09 Oct 2013
• Date of issue
  2013

History

• Received
  29 Aug 2011
• Accepted
  10 Sept 2012