Inbreeding in two maize subpopulations selected for tassel size

Endogamia em duas subpopulações de milho selecionadas para tamanho do pendão

Abstracts

The present work was based on the evaluation of full sib and S1 progenies from the maize (Zea mays L.) population ESALQ-PB1 after six cycles of divergent selection for tassel size. The following traits were analyzed: plant height , ear height, tassel branch number, tassel length, and tassel weight. For all traits, the experimental units were means of three plants per plot. Progenies were evaluated in eleven experiments (completely randomized blocks) with three replications in Piracicaba -- SP, Brazil. Means of inbred (m1) and non-inbred (m0) progenies were used to estimate inbreeding depression (I = m1 -- m0) and the contribution of homozygotes (mu0 + a*) and heterozygotes (d*) to the population mean. Considering the five traits under study, inbreeding depression varied from 1.9 to 15.9% but significance for that effect was detected only for plant height. Plant traits exhibited higher depression than tassel traits; and tassel branch number seems to be more sensitive to inbreeding than the other two tassel traits. Except for plant height, it was apparent that inbreeding depression was higher in the subpopulation negatively selected for tassel size (T--). Estimates of A = mu0 + a* and d* indicate a smaller effect of dominance deviations for all traits, as compared to the contribution of homozygotes. Significant variability among progenies was detected in most instances.

inbreeding depression; divergent selection; tassel characters

O presente trabalho foi baseado na avaliação de progênies de irmãos germanos e S1 da população de milho (Zea mays L.) ESALQ-PB1 depois de seis ciclos de seleção divergente para tamanho do pendão. Os seguintes caracteres foram analisados: altura da planta, altura da espiga, número de ramificações do pendão, comprimento do pendão e peso do pendão. Para todos os caracteres, as unidades experimentais foram médias de três plantas por parcela. As progênies foram avaliadas em onze experimentos (blocos completamente casualizados) com três repetições em Piracicaba -- SP, Brasil. As médias das progênies endogâmicas (m1) e não endogâmicas (m0) foram usadas para estimar a depressão por endogamia (I = m1 -- m0) e a contribuição de homozigotos (mi0 + a*) e heterozigotos (d*) para a média populacional. Considerando os cinco caracteres sob estudo, a depressão por endogamia variou de 1,9 to 15,9% mas somente para altura da planta foi detectada significância para aquele efeito. Os caracteres da planta exibiram maior depressão do que os caracteres do pendão; e o número de ramificações do pendão parece ser mais sensível à endogamia do que os outros caracteres do pendão. Com exceção de altura da planta, foi evidente que a depressão por endogamia foi maior na subpopulação selecionada negativamente para tamanho do pendão (T--). As estimativas de A = mi0 + a* e d* indicam um menor efeito dos desvios de dominância para todos os caracteres, quando comparados com a contribuição dos homozigotos. Foi detectada variabilidade significativa entre progênies na maioria dos casos.

depressão por endogamia; seleção divergente; caracteres do pendão

Inbreeding in two maize subpopulations selected for tassel size

Austeclinio L. de Farias Neto¹; José Branco de Miranda Filho²*

¹Embrapa Cerrados, Rodovia BR 020, km 17 - CEP: 73301-970 - Planaltina, DF.

²Depto. de Genética - USP/ESALQ, C.P. 83 - CEP: 13400-970 - Piracicaba, SP.

*Corresponding author <[e-mail]>

ABSTRACT: The present work was based on the evaluation of full sib and S₁ progenies from the maize (Zea mays L.) population ESALQ-PB1 after six cycles of divergent selection for tassel size. The following traits were analyzed: plant height , ear height, tassel branch number, tassel length, and tassel weight. For all traits, the experimental units were means of three plants per plot. Progenies were evaluated in eleven experiments (completely randomized blocks) with three replications in Piracicaba ¾ SP, Brazil. Means of inbred (m₁) and non-inbred (m₀) progenies were used to estimate inbreeding depression (I = m₁¾ m₀) and the contribution of homozygotes (m₀ + a*) and heterozygotes (d*) to the population mean. Considering the five traits under study, inbreeding depression varied from 1.9 to 15.9% but significance for that effect was detected only for plant height. Plant traits exhibited higher depression than tassel traits; and tassel branch number seems to be more sensitive to inbreeding than the other two tassel traits. Except for plant height, it was apparent that inbreeding depression was higher in the subpopulation negatively selected for tassel size (T¾). Estimates of A = m₀ + a* and d* indicate a smaller effect of dominance deviations for all traits, as compared to the contribution of homozygotes. Significant variability among progenies was detected in most instances.

Key words: inbreeding depression, divergent selection, tassel characters

Endogamia em duas subpopulações de milho selecionadas para tamanho do pendão

RESUMO: O presente trabalho foi baseado na avaliação de progênies de irmãos germanos e S₁ da população de milho (Zea mays L.) ESALQ-PB1 depois de seis ciclos de seleção divergente para tamanho do pendão. Os seguintes caracteres foram analisados: altura da planta, altura da espiga, número de ramificações do pendão, comprimento do pendão e peso do pendão. Para todos os caracteres, as unidades experimentais foram médias de três plantas por parcela. As progênies foram avaliadas em onze experimentos (blocos completamente casualizados) com três repetições em Piracicaba ¾ SP, Brasil. As médias das progênies endogâmicas (m₁) e não endogâmicas (m₀) foram usadas para estimar a depressão por endogamia (I = m₁¾ m₀) e a contribuição de homozigotos (m₀ + a*) e heterozigotos (d*) para a média populacional. Considerando os cinco caracteres sob estudo, a depressão por endogamia variou de 1,9 to 15,9% mas somente para altura da planta foi detectada significância para aquele efeito. Os caracteres da planta exibiram maior depressão do que os caracteres do pendão; e o número de ramificações do pendão parece ser mais sensível à endogamia do que os outros caracteres do pendão. Com exceção de altura da planta, foi evidente que a depressão por endogamia foi maior na subpopulação selecionada negativamente para tamanho do pendão (T¾). As estimativas de A = m₀ + a* e d* indicam um menor efeito dos desvios de dominância para todos os caracteres, quando comparados com a contribuição dos homozigotos. Foi detectada variabilidade significativa entre progênies na maioria dos casos.

Palavras-chave: depressão por endogamia, seleção divergente, caracteres do pendão

INTRODUCTION

Inbreeding is the cross between related individuals, leading to an increase in the frequency of homozygotes and a decrease of heterozygotes. The negative effect of inbreeding, known as inbreeding depression, is a reduction in the mean of quantitative traits, related either to reproduction capacity or physiological efficiency, and is a consequence of recessive deleterious alleles (Falconer, 1964; Miranda Filho, 1999).

Under a breeding viewpoint, inbreeding can be useful either for the development of inbred lines to be used in hybrid crosses or in recurrent selection with inbred progenies for the development of improved populations (Paterniani & Miranda Filho, 1987; Hallauer & Miranda Filho, 1995). Cockerham (1983) showed that recurrent selection under inbreeding could be affected negatively by the component D₁, that is the covariance between additive and dominance effects in the homozygotes. In fact, because D₁ is a covariance it can be negative for alleles at low frequency, and then affect negatively the gain from selection (Lamkey & Hallauer, 1992). On the other hand, recurrent selection under inbreeding allows selection against deleterious and lethal or sublethal recessives (Hallauer, 1980). This aspect is particularly important in Brazil, where many broad base maize populations have exhibited a high genetic load, resulting in a high inbreeding depression when submitted to inbreeding (Lima et al., 1984; Marques, 1988).

Hallauer & Miranda Filho (1995) pointed out that despite the extremely high number of inbred lines developed in maize throughout the world, estimates of inbreeding depression for several quantitative traits are surprisingly low. The same authors reported inbreeding depression of 57.6 grams per plant for grain yield for 50% expected homozygosity, on the average of several estimates; for plant height and ear height, average inbreeding depressions were 22.0 cm and 15.6 cm, respectively. Lima et al. (1984) reported inbreeding depression in 32 Brazilian maize populations, varying from 27.0 to 59.9% for grain yield, 6.6 to 20.3% for plant height and 6.9 to 27.4% for ear height. They also observed that populations derived from inbred lines exhibited lower inbreeding depression (34% less) than open-pollinated varieties that were never submitted to inbreeding, thus corroborating Hallauer (1980) on the effectiveness of inbreeding for selection against recessive deleterious.

The inbreeding effects on tassel size (tassel branch number) were estimated by Kassouf & Miranda Filho (1986) as 15.3% in the maize population ESALQ-PB1 and by Nass & Miranda Filho (1995) as 19.4% and 12.7% in two semi-exotic populations. In this work, full-sib and inbred (S₁) progenies of two subpopulations divergently selected for tassel size were used for estimating the effects of inbreeding on tassel and plant characters.

MATERIAL AND METHODS

The base population ESALQ-PB1 was submitted to six cycles of divergent selection for tassel size, thus generating two subpopulations, which were designated by ESALQ-PB1 (T+) and ESALQ-PB1 (T¾) for increasing and decreasing the trait, respectively. ESALQ-PB1 is a composite formed from crosses between seven short plant varieties (Miranda Filho, 1974). The divergent selection program initiated in 1979/80 in Piracicaba, São Paulo State, Brazil, based on the performance of 147 half-sib families evaluated in three 7x7-lattice experiments with four replications. The following five cycles were completed under mass selection for both sexes (biparental crosses between selected plants). In the sixth cycle, selfing was also used for the development of S₁ progenies besides de full-sib progenies obtained in the biparental crosses. The four sets and their respective number of progenies are shown in TABLE 1.

The total number of progenies was divided into several experiments following the completely randomized block design with three replications in Piracicaba - SP, Brazil, in November/1994. Plots were 4 m long under spacing of 0.90m between rows and 0.20 m between plants within rows. The following traits were analyzed: plant height (cm), ear height (cm), tassel branch number, tassel length (cm), and tassel weight (g). For all traits, the experimental units were plot means of three plants per plot. The commercial hybrid G-85 was used as check.

For each experiment, the analysis of variance followed the model of the completely randomized block design, i.e., Y_ij = m + p_i + b_j + e_ij , where Y_ij is the observed mean of the i^th progeny in the j^th replication; p_i is a random progeny effect; b_j is a random block effect; and e_ij is the experimental error term, assumed to be normally distributed with mean 0 and variance s² for testing hypothesis through the F-test. Pooling the respective sums of squares combined the individual analyses representing the same subpopulation.

Inbreeding depression (I) for each trait was obtained by the contrast I = m₁ -m₀, where m₁ and m₀ are population means represented by inbred (S₁) and non-inbred (full-sib) progenies, respectively. Also, m₀ = m₀ + a* + d* and m₁ = m₀ + a* + d* ; m₀ = represents the mean of homozygotes for each locus summed over all loci; a* is the overall contribution (deviation from m₀) of the homozygous genotypes; and d* is the overall contribution of heterozygotes. So, the quantities A = m₀ + a* and d* were estimated by  = 2 m₁ - m₀ and * = 2 (m₀ - m₁)

RESULTS AND DISCUSSION

For all traits, means of inbred (S₁) progenies were smaller than for non-inbred (full-sib) progenies. This phenomenon is known as inbreeding depression and depends directly on the level of dominance so that no changes in the mean are expected under inbreeding when genes controlling the trait follow a strictly additive model (Falconer, 1964). Therefore, a higher depression under inbreeding is expected for traits under the control of higher levels of dominance. Considering the five traits under study, inbreeding depression varied from 1.9 to 15.9% but significance for that effect was detected only for plant height (TABLE 2). It is also clear that plant traits exhibited higher depression than tassel traits; and tassel branch number seems to be more sensitive to inbreeding than the other two tassel traits. Except for plant height, it was apparent that inbreeding depression was higher in the subpopulation negatively selected for tassel size (T¾). Such effects may limit the use of the subpopulation ESALQ-PB1 (T¾) as source of inbred lines to be used in hybrid crosses, mainly if the lines are to be used as male parents. Nevertheless, Stebbins (1957) pointed out that the inbreeding effect in cross-pollinated species is higher in the first generations and tend to decrease in the next generations of selfing. This is a consequence of the reduction in the genetic load represented by recessive deleterious and semi-lethal genes that are brought about by inbreeding (Miranda Filho, 1999). It was also expected that inbreeding depression should be smaller in the divergently selected subpopulations, as a consequence of the increase in the level of homozygosity caused by selection, when compared with the original population.

Kassouf & Miranda Filho (1986) also reported inbreeding depression of 15.3% for tassel size in the base population ESALQ-PB1. Nass & Miranda Filho (1995) reported on inbreeding depression of the order of 13.7 and 12.8% for plant height, 15.9 and 14.2% for ear height and 19.4 and 12.7% for tassel branch number in the semi-exotic populations EE-1 and EC-4, respectively. A smaller depression for tassel branch number (11.1%) was reported by San Vicente & Hallauer (1993). Hallauer & Miranda Filho (1995) reported on inbreeding depression of 10% for plant height and 15% for ear height for 50% expected homozygosity in the maize population BSSS.

Miranda Filho & Andrade (2000) estimated means of thirteen quantitative traits in the original population ESALQ-PB1. Although the results are not directly comparable, tassel weight (5.87 g) and tassel length (40.9 cm) did not differ greatly from the means shown in TABLE 2. However, tassel branch number was strongly affected by the divergent selec-tion for tassel size. In fact, the original population mean of 18.4 (Miranda Filho & Andrade, 2000) changed to 35.2 (non-inbred) and 32.2 (inbred) in the positive selection (T+) and to 7.4 (non-inbred) and 6.3 (inbred) in the negative selection (T¾).

Estimates of A = m₀ + a* (expected mean of a random sample of completely homozygous lines) and d* (overall contribution of heterozygotes to the mean), indicate a smaller effect of dominance deviations for all traits, as compared to the contribution of homozygotes (TABLE 2).

A similar conclusion was reported by Nass & Miranda Filho (1995), except for yield traits where the effects of dominance were more expressive, although epistatic effects in depression caused by strongly deleterious alleles cannot be ruled out.

A complementary set of information referred to the observed variability among progenies, which showed to be significant (P < 0.01) in most of the instances (TABLE 3). Exceptions representing non-significant variation were due to ear height in the subpopulation ESALQ-PB1 (T+)S₁ and for tassel weight in the subpopulation ESALQ-PB1 (T-)FS. A small variability should be expected in some instances, as a consequence of the effect of six cycles of divergent selection for tassel size. Apparently, for plant height and ear height, a higher expression of genetic variability occurred in the inbred progenies, although higher environmental effects represented by the error mean squares were also detected in that cases. A very high difference in the expression of variability was observed for tassel branch number, the trait that was submitted to divergent selection. For both types of progenies the higher mean squares were for the subpopulation selected for larger tassels. Because the large difference between means of divergently selected populations, a scale effect on the magnitude of the variances (mean squares) must be taken into account. No great differences in the expression of variability were observed for the other tassel traits.

Received October 13, 1999

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Publication Dates

• Publication in this collection
  21 Sept 2000
• Date of issue
  Sept 2000

History

• Received
  13 Oct 1999