Características hematológicas de teleósteos brasileiros: II. Parâmetros sangüíneos do Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) em policultivo intensivo

Tavares-Dias, Marcos; Tenani, Rosilene A; Gioli, Leonardo D; Faustino, Christiane D

doi:10.1590/S0101-81751999000200008

Resumo

Hematological parameters of freshwater fish Piaractus mesopotamicus (Holmberg, 1887) kept in an intensive polyculture system were determined. The correlations among these parameters as well as those between the parameters and the biometric data (total weight and standard length) were also analyzed. Erythrocytes count was 1.708±0.42 x 10(6)/µl, hemoglobin 6.6±2.0 g% of blood and hematocrit 32.5±4.6%, mean corpuscular volume (MCV) 209.8±69.5%, mean corpuscular hemoglobin (MCH) 40.2±5.6 pg and mean corpuscular hemoglobin concentration (MCHC) 21.4±8.4%. The percentage of defense blood cells including leucocytes and thrombocytes, was studied. The percentage of thrombocytes was 61.4±10.0%, lymphocytes 21.7±1.8%,neutrophils 10.4±11.0%, monocytes 4.7±3.3% and special granulocitic cells 1.8±2.0%. The number of erythrocytes was positively correlated with the hemoglobin rate and negatively correlated with MVC. Hemoglobin was positively correlated with MCHC whereas hematocrit was negatively correlated with MCHC and MVC. Contrarily, leukocytes did not show correlations among themselves or with the hematological parameters and biometric data.

Piaractus mesopotamicus; blood; erythrocytes; freshwater fish; leucocytes; thrombocytes

Características hematológicas de teleósteos brasileiros. II. Parâmetros sangüíneos do Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) em policultivo intensivo

Hematological characteristics of Brazilian teleosts. II. Blood parameters of Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) in an intensive polyculture system

Marcos Tavares-Dias^I,II; Rosilene A. Tenani^I; Leonardo D. Gioli^I; Christiane D. Faustino^I

^IDepartamento de Ciências Biológicas, Universidade de Franca. Avenida Dr. Armando Salles Oliveira 201, 14404-600 Franca, São Paulo, Brasil

^IILaboratório de Patologia de Organismos Aquáticos, Centro de Aqüicultura, Universidade Estadual Paulista. Rodovia Carlos Tonanni Km 05, 14870-000 Jaboticabal, São Paulo, Brasil

ABSTRACT

Hematological parameters of freshwater fish Piaractus mesopotamicus (Holmberg, 1887) kept in an intensive polyculture system were determined. The correlations among these parameters as well as those between the parameters and the biometric data (total weight and standard length) were also analyzed. Erythrocytes count was 1.708±0.42 x 10⁶/µl, hemoglobin 6.6±2.0 g% of blood and hematocrit 32.5±4.6%, mean corpuscular volume (MCV) 209.8±69.5%, mean corpuscular hemoglobin (MCH) 40.2±5.6 pg and mean corpuscular hemoglobin concentration (MCHC) 21.4±8.4%. The percentage of defense blood cells including leucocytes and thrombocytes, was studied. The percentage of thrombocytes was 61.4±10.0%, lymphocytes 21.7±1.8%,neutrophils 10.4±11.0%, monocytes 4.7±3.3% and special granulocitic cells 1.8±2.0%. The number of erythrocytes was positively correlated with the hemoglobin rate and negatively correlated with MVC. Hemoglobin was positively correlated with MCHC whereas hematocrit was negatively correlated with MCHC and MVC. Contrarily, leukocytes did not show correlations among themselves or with the hematological parameters and biometric data.

Key words:Piaractus mesopotamicus, blood, erythrocytes, freshwater fish, leucocytes, thrombocytes

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Recebido em 10.X.1997; aceito em 29.III.1999.

Aldrin, J.F.; J.L. Messager; F. Baudin Laurencin. 1982.La biochimie clinique en aquaculture. Interet et perspective. CNEXO Actes Colloq.14:219-326.
Al-Hassan, L.A.J.; H.K. Ahmed & S.A. Majeed. 1993. Some haematological parameters in relation to the biology of the fish Acanthopagrus latus. Jour. Environ. Sci. Health 28A (7):1599-1611.
Banzato, D.A. & S.N. Kronka. 1995. Experimentação agrícola. Jaboticabal, Ed. Funep, 3ª ed., 247p.
Carter, G.S. 1935. Respiratory adaptation of the fishes of the forest water, with description of the acessory organs of Eletrophorus eletricus (Linn.) and Plecostomus plecostomus (Linn.). Jour. Linn. Soc. Lond. Zool. 39:219.
Castagnolli, N.1992. Piscicultura de água doce. Jaboticabal, Ed. Funep, 189p.
Chaudhuri, S.H.; T. Pandit & S. Banerjee. 1986. Size and sex related variations of some blood parameters of Sarotherodon mossambica. Environ. Ecol. 4(1):61-63.
Chondar, S.L. 1982.The haematology of Gudusia chapra (Pisces: Clupeidae). Jour. Inland Fish. Soc. India. 14 (1):1-10.
Collier, H.B. 1944. The standardization of blood haemoglobin determinations. Can. Med. Ass. Jour. 50:550-552.
Favaretto, A.L.V.; R.A. Lopes; S.O. Petenusci & P. Sawaya. 1978. Estudo morfométrico de eritrócitos de Plecostomus regani (Pisces, loricariidae). Teleósteo de respiração aquática e aérea, após exposição ao ar atmosférico. Rev. Fac. Farm. Odont., Ribeirão Preto, 15:59-66.
Goldenfarb, P.B.; F.P. Bowyer; Hall & E. Brosious. 1971. Reproducibility in the hematology laboratory: the microhematocrit determination. Amer. Jour. Clin. Path. 56:35-39.
Grecchi, R.; A.M. Saliba & M. Mariano. 1980. Morphological changes, surface receptors and phagocytic potencial of fowl mononuclear phagocytes and trombocytes in vivo and in vitro. Jour. Pathol. 130:23-31.
Hickey Jr., C.R.. 1982. Comparative hematology of wild and captive cunners. Trans. Amer. Fish. Soc. 111 (2):242-249.
Houston, A.H.; N. DOBRIC & R. Kahurananga. 1996. The nature of hematological response in fish. Fish Physiol. Biochem. 15 (4):339-347.
Ishida, Y.; M. Sugyiama; H. Kajigaya; M. Umedea & M. Isoda. 1985. Inflammatory reactions on Japanese quails muscular tissue caused by sutures. Bull. Nippon Vet. Zoot. Col. 31:39-46.
Kajigaya, H.; M. Kamekura; N. Tanahara; A. Otha; H. Suzuki; M. Sugiyama & M. Isosa. 1985. Acute and chronic inflammation induced by silk sutures in chicken mesentery. Bull. Nippon Vet. Zoot. Col. 34:35-8.
Kavamoto, E.T.; M. Tokumaru; R.A.P. Souza e Silva & B.E.S. Campos. 1983. Algumas variáveis hematológicas do "cascudo" Plecostomus albopunctatus (Regan, 1908). Bol. Inst. Pesca, São Paulo, 10:101-106.
Larsson, A.; M.J. Johansson-Sjobeck & R. Fange. 1976. Comparative study of some haematological and biochemical blood parameters in fishes from Shagerrak. Jour. Fish Biol. 9:425-440.
Lea Master, B.R.; J.A. Brock; R.S. Fujioka & R.M. Nakamura. 1990. Hematologic and blood chemistry values for Sarotherodon melanotheron and a red hybrid tilapia in freshwater and seawater. Comp. Biochem. Physiol. 97A (4):525-529
Martins, M.L.; N. Castagnolli; S.M.F. Zuim & E.C. Urbinati. 1995. Influência de diferentes níveis de vitamina C na ração sobre parâmetros hematológicos de alevinos de Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae). Revta bras. Zool. 12 (3):609-618.
Matushima, E.R. & M. Mariano. 1996. Kinetics of the inflammatory reaction induced by carrageenin in the swinbladder of Oreochromis niloticus (Nile tilapia). Braz. Jour. Vet. Anim. Sci. 33 (1):5-10.
Murray, S.A. 1984. Hematological study of the bluegill, Lepomis macrochirus Raf. Comp. Biochem. Physiol. 78A (4):787-791.
Pérez, J.E.; A. Boaba & G. Ojeda. 1984. Blood parameters in fishes. III. Hemoglobin concentration, hematocrit, and th number of red blood cells in some freshwater fishes of eastern Venezuela. Bol. Inst. Oceanogr. Venezulea. Univ. Oriente 23:43-47.
Ranzani-Paiva, M.J.T. 1991a. Características sangüíneas da pirapitinga do sul, Brycon sp, sob condições experimentais de criação intensiva. Braz. Jour. Vet. Res. Anim. Sci., São Paulo, 28 (2):141-153.
______. 1991b. Hematologia de peixes; p.65-70. In: H.S.L. SANTOS (Ed.). Histologia de Peixes. São Paulo, FCAV-UNESP, 83p.
______. 1995. Características hematológicas de tainha Mugil platanus Günther, 1880 (Osteichthyes, Mugilidae) da região estutário-laguna de Cananéia-SP. (Lat. 25º00'S - Long. 47º55'W). Bol. Inst. Pesca, São Paulo, 22 (1):1-22.
Ranzani-Paiva, M.J.; C.M. Ishikawa; M.C Portella & R.J. Celiberto. 1987. Hematologia da carpa comum Cyprimts carpio, infestada por Argidus sp. e após um tratamento com fosfato de 0,0-dimetil-oxi-2,2,2,-tricloroetilo (Neguvon). Bol. Inst. Pesca, São Paulo, 14:83-92.
Ranzani-Paiva, M.J.T. & H.M. Godinho. 1983. Sobre células sangüíneas e contagem diferencial de leucócilos e eritroblastos em curimbatá, Prochilodus scrofa Steindacher, 1881 (Osteichthyes, Cypriniformes, Prochilodontidae). Rev. Brasil. Biol. 43 (4):331-338.
Ribeiro, W.R. 1991. Leucócitos de peixes, p.61-64. In: H.S.L. Santos (Ed.). Histologia de Peixes. São Paulo, FCAV-UNESP, 83p.
Rosenfeld, G. 1947. Corânte pancrômico para hematologia e citologia clínica. Nova combinação dos componentes do May-Grunwald e do Giensa num só corante de emprego rápido. Mem. Inst. Butantan 20:329-334.
Satake, T.; A. Nutti-Sobrinho; O.V. Paula-Lopes; R.A. Lopes & H.S.L. Santos. 1986. Haematological study brazilian fish. III. Blood parameters in armored catfishe Hypostomus paulinus Ihering 1905 (Pisces, Loricariidae). Ars Veterinaria 2 (2):179-183.
Suzuki, K. 1986. Morphological and phagocytic characteristics of peritoneal exudate cells in tilapia, Oreochromis niloticus (Trewavas), and carp, Cyprinus carpio L. Jour. Fish Biol. 29 (3):349-364.
Tavares-Dias, M.; E.F.S. Sandrim & A. Sandrim. 1998 Características Hematológicas do tambaqui (Colossoma Macropomum) Cuvier, 1818 (Osteichthyes: Characidae) em sistema de monocultivo intensivo. I. Série eritrocitária. Rev. Brasil. Biol. 58 (2):197-202.
Torres-Filho, I.P.; E.G. Moura; C.C.A. Nascimento; J. Contaifer; C.F. Ramos; M.A. Pimenta & E.B. Torres. 1986. Parâmetros hematológicos do cascudo (Hypostomus punctatus). Ciênc. Cult. 38:825-832.
Wintrobe, M.M. 1934. Variations on the size and haemoglobin content of erythrocytes in the blood various vertebrates. Foglia Haematol. 51:32-49.
Zuim, S.M.F.; A.A.M. Rosa & N. Castagnolli. 1988. Influence of sex and environment on metabolic parameters of pacu Piaractus mesopotamicus (Holmberg, 1887) during final maturation stage. Bull. Canad. Aquacult. Assoc. Proc., Vancouver, 88 (4):55-56.

Datas de Publicação

Publicação nesta coleção
01 Jul 2009
Data do Fascículo
Jun 1999

Histórico

Recebido
10 Out 1997
Aceito
29 Mar 1999

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Aldrin, J.F.; J.L. Messager; F. Baudin Laurencin. 1982.La biochimie clinique en aquaculture. Interet et perspective. CNEXO Actes Colloq.14:219-326.

[2] Al-Hassan, L.A.J.; H.K. Ahmed & S.A. Majeed. 1993. Some haematological parameters in relation to the biology of the fish Acanthopagrus latus. Jour. Environ. Sci. Health 28A (7):1599-1611.

[3] Banzato, D.A. & S.N. Kronka. 1995. Experimentação agrícola. Jaboticabal, Ed. Funep, 3ª ed., 247p.

[4] Carter, G.S. 1935. Respiratory adaptation of the fishes of the forest water, with description of the acessory organs of Eletrophorus eletricus (Linn.) and Plecostomus plecostomus (Linn.). Jour. Linn. Soc. Lond. Zool. 39:219.

[5] Castagnolli, N.1992. Piscicultura de água doce. Jaboticabal, Ed. Funep, 189p.

[6] Chaudhuri, S.H.; T. Pandit & S. Banerjee. 1986. Size and sex related variations of some blood parameters of Sarotherodon mossambica. Environ. Ecol. 4(1):61-63.

[7] Chondar, S.L. 1982.The haematology of Gudusia chapra (Pisces: Clupeidae). Jour. Inland Fish. Soc. India. 14 (1):1-10.

[8] Collier, H.B. 1944. The standardization of blood haemoglobin determinations. Can. Med. Ass. Jour. 50:550-552.

[9] Favaretto, A.L.V.; R.A. Lopes; S.O. Petenusci & P. Sawaya. 1978. Estudo morfométrico de eritrócitos de Plecostomus regani (Pisces, loricariidae). Teleósteo de respiração aquática e aérea, após exposição ao ar atmosférico. Rev. Fac. Farm. Odont., Ribeirão Preto, 15:59-66.

[10] Goldenfarb, P.B.; F.P. Bowyer; Hall & E. Brosious. 1971. Reproducibility in the hematology laboratory: the microhematocrit determination. Amer. Jour. Clin. Path. 56:35-39.

[11] Grecchi, R.; A.M. Saliba & M. Mariano. 1980. Morphological changes, surface receptors and phagocytic potencial of fowl mononuclear phagocytes and trombocytes in vivo and in vitro. Jour. Pathol. 130:23-31.

[12] Hickey Jr., C.R.. 1982. Comparative hematology of wild and captive cunners. Trans. Amer. Fish. Soc. 111 (2):242-249.

[13] Houston, A.H.; N. DOBRIC & R. Kahurananga. 1996. The nature of hematological response in fish. Fish Physiol. Biochem. 15 (4):339-347.

[14] Ishida, Y.; M. Sugyiama; H. Kajigaya; M. Umedea & M. Isoda. 1985. Inflammatory reactions on Japanese quails muscular tissue caused by sutures. Bull. Nippon Vet. Zoot. Col. 31:39-46.

[15] Kajigaya, H.; M. Kamekura; N. Tanahara; A. Otha; H. Suzuki; M. Sugiyama & M. Isosa. 1985. Acute and chronic inflammation induced by silk sutures in chicken mesentery. Bull. Nippon Vet. Zoot. Col. 34:35-8.

[16] Kavamoto, E.T.; M. Tokumaru; R.A.P. Souza e Silva & B.E.S. Campos. 1983. Algumas variáveis hematológicas do "cascudo" Plecostomus albopunctatus (Regan, 1908). Bol. Inst. Pesca, São Paulo, 10:101-106.

[17] Larsson, A.; M.J. Johansson-Sjobeck & R. Fange. 1976. Comparative study of some haematological and biochemical blood parameters in fishes from Shagerrak. Jour. Fish Biol. 9:425-440.

[18] Lea Master, B.R.; J.A. Brock; R.S. Fujioka & R.M. Nakamura. 1990. Hematologic and blood chemistry values for Sarotherodon melanotheron and a red hybrid tilapia in freshwater and seawater. Comp. Biochem. Physiol. 97A (4):525-529

[19] Martins, M.L.; N. Castagnolli; S.M.F. Zuim & E.C. Urbinati. 1995. Influência de diferentes níveis de vitamina C na ração sobre parâmetros hematológicos de alevinos de Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae). Revta bras. Zool. 12 (3):609-618.

[20] Matushima, E.R. & M. Mariano. 1996. Kinetics of the inflammatory reaction induced by carrageenin in the swinbladder of Oreochromis niloticus (Nile tilapia). Braz. Jour. Vet. Anim. Sci. 33 (1):5-10.

[21] Murray, S.A. 1984. Hematological study of the bluegill, Lepomis macrochirus Raf. Comp. Biochem. Physiol. 78A (4):787-791.

[22] Pérez, J.E.; A. Boaba & G. Ojeda. 1984. Blood parameters in fishes. III. Hemoglobin concentration, hematocrit, and th number of red blood cells in some freshwater fishes of eastern Venezuela. Bol. Inst. Oceanogr. Venezulea. Univ. Oriente 23:43-47.

[23] Ranzani-Paiva, M.J.T. 1991a. Características sangüíneas da pirapitinga do sul, Brycon sp, sob condições experimentais de criação intensiva. Braz. Jour. Vet. Res. Anim. Sci., São Paulo, 28 (2):141-153.

[24] ______. 1991b. Hematologia de peixes; p.65-70. In: H.S.L. SANTOS (Ed.). Histologia de Peixes. São Paulo, FCAV-UNESP, 83p.

[25] ______. 1995. Características hematológicas de tainha Mugil platanus Günther, 1880 (Osteichthyes, Mugilidae) da região estutário-laguna de Cananéia-SP. (Lat. 25º00'S - Long. 47º55'W). Bol. Inst. Pesca, São Paulo, 22 (1):1-22.

[26] Ranzani-Paiva, M.J.; C.M. Ishikawa; M.C Portella & R.J. Celiberto. 1987. Hematologia da carpa comum Cyprimts carpio, infestada por Argidus sp. e após um tratamento com fosfato de 0,0-dimetil-oxi-2,2,2,-tricloroetilo (Neguvon). Bol. Inst. Pesca, São Paulo, 14:83-92.

[27] Ranzani-Paiva, M.J.T. & H.M. Godinho. 1983. Sobre células sangüíneas e contagem diferencial de leucócilos e eritroblastos em curimbatá, Prochilodus scrofa Steindacher, 1881 (Osteichthyes, Cypriniformes, Prochilodontidae). Rev. Brasil. Biol. 43 (4):331-338.

[28] Ribeiro, W.R. 1991. Leucócitos de peixes, p.61-64. In: H.S.L. Santos (Ed.). Histologia de Peixes. São Paulo, FCAV-UNESP, 83p.

[29] Rosenfeld, G. 1947. Corânte pancrômico para hematologia e citologia clínica. Nova combinação dos componentes do May-Grunwald e do Giensa num só corante de emprego rápido. Mem. Inst. Butantan 20:329-334.

[30] Satake, T.; A. Nutti-Sobrinho; O.V. Paula-Lopes; R.A. Lopes & H.S.L. Santos. 1986. Haematological study brazilian fish. III. Blood parameters in armored catfishe Hypostomus paulinus Ihering 1905 (Pisces, Loricariidae). Ars Veterinaria 2 (2):179-183.

[31] Suzuki, K. 1986. Morphological and phagocytic characteristics of peritoneal exudate cells in tilapia, Oreochromis niloticus (Trewavas), and carp, Cyprinus carpio L. Jour. Fish Biol. 29 (3):349-364.

[32] Tavares-Dias, M.; E.F.S. Sandrim & A. Sandrim. 1998 Características Hematológicas do tambaqui (Colossoma Macropomum) Cuvier, 1818 (Osteichthyes: Characidae) em sistema de monocultivo intensivo. I. Série eritrocitária. Rev. Brasil. Biol. 58 (2):197-202.

[33] Torres-Filho, I.P.; E.G. Moura; C.C.A. Nascimento; J. Contaifer; C.F. Ramos; M.A. Pimenta & E.B. Torres. 1986. Parâmetros hematológicos do cascudo (Hypostomus punctatus). Ciênc. Cult. 38:825-832.

[34] Wintrobe, M.M. 1934. Variations on the size and haemoglobin content of erythrocytes in the blood various vertebrates. Foglia Haematol. 51:32-49.

[35] Zuim, S.M.F.; A.A.M. Rosa & N. Castagnolli. 1988. Influence of sex and environment on metabolic parameters of pacu Piaractus mesopotamicus (Holmberg, 1887) during final maturation stage. Bull. Canad. Aquacult. Assoc. Proc., Vancouver, 88 (4):55-56.

Brasil

Brasil

Características hematológicas de teleósteos brasileiros: II. Parâmetros sangüíneos do Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) em policultivo intensivo

Hematological characteristics of Brazilian teleosts: II. Blood parameters of Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) in an intensive polyculture system

Resumo

Datas de Publicação

Histórico