Description of the larva of Lopesia spinosa Maia (Diptera, Cecidomyiidae) and new occurrences of the species

ABSTRACT

The hitherto unknown larva of Lopesia spinosa Maia (Diptera: Cecidomyiidae) is described and the geographical distribution of the species is extended in Brazil to Delfinópolis in Minas Gerais; Altinópolis and Jundiaí in São Paulo. Diagnostic characters of the species and illustration of the larva are presented.

Keywords:
Croton floribundus; Gall maker; Geographical distribution; Neotropical region; Taxonomy

Galls were found on leaf of Croton floribundus Spreng. (Euphorbiaceae) in São Paulo (Neves et al., 2019; Gomes et al., 2013) and Minas Gerais States (Urso-Guimarães et al., 2003) that appeared typical of Lopesia spinosaMaia, 2004 found in Tiradentes, Minas Gerais State (Maia and Fernandes, 2004, Fig. 34). The specimens are L. spinosa are recognized by the presence of the diagnostic characters of the species, sclerotized facial processes (Maia, 2004, Fig. 48) and 2-toothed antennal horn of pupa (Maia, 2004, Fig. 49). C. floribundus is a pioneer species with wide distribution in Brazil (Alagoas, Bahia, Ceará, Paraíba, Pernambuco, Distrito Federal, Mato Grosso, Mato Grosso do Sul, Espírito Santo, Minas Gerais, Rio de Janeiro, São Paulo, and Paraná) and Paraguay (Guadalupe) (Dos Santos et al., 2017).

I reared all stages of the life cycle of L. spinosa, including the third stage of the unknown larva. For completeness the description of this species, the larva is described and illustrated here with new records of geographical distribution.

Material and methods

Branches of C. floribundus (Euphorbiaceae) with galls were collected in Altinópolis, State of São Paulo, Brazil (Neves et al., 2019, Fig. 1A). Additional material was collected in Delfinópolis, Minas Gerais State (Urso-Guimarães et al., 2003, Fig. 12) and in Serra do Japi Reserve, Jundiaí, São Paulo State (Gomes et al., 2013, Fig. 2). Data of collection and emergency are in the section “Examined material". Some galls were opened to obtain immature specimens, and other galls were maintained in plastic pots for rearing of adults. The specimens were preserved in 80% ethanol. Part of the specimens was later mounted on slides following the methodology outlined by Gagné (1994), and identified using literature for comparison. Exsiccates are deposited in the Herbarium of UFSCar – Sorocaba (SORO). Specimens of gall makers are deposited in the Diptera collection of the Museu de Zoologia da Universidade de São Paulo/USP (MZSP).

Fig. 1
Larva of Lopesia spinosa. A. Cephalic and thoracic region, ventral view. B. Detail of the semicircular spatula and lateral papillae. C. Terminal segment, dorsal view.

Results and discussion

Lopesia Rübsaamen, 1908

Lopesia spinosa Maia, 2004

Description – Third Instar Larva (Fig. 1A–C)

Body length: 2.00–2.06 mm long (n = 5). Fusiform and cylindrical body; integument rough with micro-spines. Cephalic and thoracic region (Fig. 1A): Antenna short; spatula replaced by two evident sclerotized semicircles (Fig. 1B) in the three thoracic segments (n = 5), two groups of three lateral papillae on each side of spatula, three pairs setose in each group. Terminal segment round with four pairs of terminal papillae (Fig. 1C): three pairs corniform, and one setose, anus ventral in shaft.

Examined material

BRAZIL, São Paulo State: Altinópolis, Touristic Complex of Itambé (21°04′02.7″S 47°26′12.3″W, new record). Collection date, 15.iii.2000 – Slide mounted: 1 male – Emergency date, 20.iii.2000; 4 females – Emergency date, 21.iii.2000; 1 female – Emergency date, 22.iii.2000; 5 exuviae and 5 larvae. In vials: 1 female – Emergency date, 15.iii.2000; 7 females – Emergency date, 16.iii.2000; 18 females – Emergency date, 20.iii.2000; 13 females – Emergency date, 21.iii.2000; 46 exuviae; 4 pupae. Urso- Guimarães, M.V.; Balbi, M.I.P.A. & Mendes, H.F. col. Additional material: BRAZIL, São Paulo State: Jundiaí, Serra do Japi Reserve (23°39′58.8″S, 46°53′08.7″W, new record): Slide mounted: Collection date, 08.ii.2011 – 1 male – Emergency date, 10.ii.2011; 1 male – Emergency date, 13.ii.2011; 1 female – Emergency date, 10.ii.2011; 1 female – Emergency date, 11.ii.2011; 1 female – Emergency date, 13.ii.2011; 1 exuvia. Gomes, G. C. col. BRAZIL, Minas Gerais State: Delfinópolis (20° 12′44.4″S, 46°55′15.1″W, new record): Collection date, 29.vi.2000. Galls. Urso-Guimarães, M.V.; Bonifácio-Silva, A.C. & Scareli-Santos, C. col.

Remarks of the larvae of Lopesia spinosa

The larvae of Lopesia species are characterized by short terminal setae, most of which are corniform and in a terminal projection (Gagné and Marohasy, 1993; Gagné, 1994). The Lopesia species presents a variation in the number of teeth of the spatula, sometimes with reduction of the number of teeth or lacking of the spatula. The cases of reduction of spatula are L. aldinae with one tooth and L. niloticae, L. armata, L. quadrata, and L. singularis which spatula is lacking. The larva of L. spinosa has two sclerotized semicircles replacing the spatula in the three thoracic segments, a unique case in Lopesia. The terminal segment of L. spinosa is round and presents four terminal papillae, three corniform of equal size and one setiform longer then the corniform. The other species with terminal segment round and unequal length of terminal papillae are L. erythroxyli, L. licaniae, and L. niloticae (Rodrigues and Maia, 2010), but in L. niloticae the spatula is lack and the terminal segment has reentrancy and in L. erythroxyli and L. licaniae the spatula has two teeth and a long shaft.

Updated geographical distribution of Lopesia spinosa

Once the galls and specimens of the Lopesia spinosawere sampled in Altinópolis, São Paulo State (Neves et al., 2019), and additional material was sampled in Serra do Japi Reserve in São Paulo State (Gomes et al., 2013) and Delfinópolis in Minas Gerais State (Urso-Guimarães et al., 2003), the geographical distribution is herein extended from Tiradentes, Minas Gerais State (Maia and Fernandes, 2004) to these localities. As the host plant species is widely distributed in Brazil (Alagoas, Bahia, Ceará, Paraíba, Pernambuco, Distrito Federal, Mato Grosso, Mato Grosso do Sul, Espírito Santo, Minas Gerais, Rio de Janeiro, São Paulo, and Paraná) and Paraguay (Guadalupe) (Dos Santos et al., 2017), it is also expected that Lopesia spinosa occurs in other regions where the plant is distributed. Further investigation is needed to confirm this hypothesis.

Acknowledgements

The author acknowledges support from Dr. Carlos J.E. Lamas for providing access to the facilities in MZUSP; Maria Isabel Protti de Andrade Balbi and Humberto Fonseca Mendes for helping in the field work; Gabriela C. Gomes for providing the specimens of Serra do Japi Reserve and Dr. Ana Carolina Bonifácio-Silva (ICMBio) for identifying the species of host plant. The collection of material was supported from FAPESP Proc. #99/01429-1.

References

• Dos Santos, R.F., Riina, R., Caruzo, M.B.R., 2017. Diversity of arborescent lineages of Crotoneae (Euphorbiaceae) in the Brazilian Atlantic Rain Forest. Plant Syst. Evol. 303, 1467-1497.
• Gagné, R.J., 1994. The Gall Midges of the Neotropical Region. Cornell University Press, Ithaca, pp. 352.
• Gagné, R.J., Marohasy, J., 1993. The gall midges (Diptera: Cecidomyiidae) of Acacia spp (Mimosaceae) in Kenya. Insecta Mundi 7, 77-124.
• Gomes, G.C., Franco, M.S., Hansson, C., 2013. Uma nova espécie de Omphale Haliday 1833 (Hymenoptera: Eulophidae) do Brasil, parasitoide de galhas em Croton floribundus Spreng (Euphorbiaceae). Biota Neotropica 13, 34-37.
• Maia, V.C., 2004. A New Genus and Six New Species of Gall Midges (Diptera, Cecidomyiidae) from Serra de São José, vol. 62. Arquivos do Museu Nacional, Minas Gerais State Brazil, pp. 69–82.
• Maia, V.C., Fernandes, G.W., 2004. Galhas de insetos da Serra de São José (Tiradentes, MG Brasil). Braz. J. Biol. 64, 423-445.
• Neves, A.R., Balbi, M.I.P.A., Urso-Guimarães, M.V., 2019. Characterization of insect galls from a vegetation area in Altinópolis, São Paulo State, Brazil. Pap. Avulsos Zool. 59, e20195904.
• Rodrigues, A.R., Maia, V.C., 2010. Duas novas espécies de Lopesia Rübsaamen (Diptera Cecidomyiidae) do Brasil, com chave para as espécies. Biota Neotropica 10, 85-99.
• Urso-Guimarães, M.V., Scareli-Santos, C., Bonifácio, A.C.C., 2003. Occurrence and characterization of entomogen galls in plants from natural vegetation areas in Delfinópolis, MG, Brazil. Braz. J. Biol. 63, 705-715.

Associate Editor: Marcia Souto Couri

Publication Dates

• Publication in this collection
  05 Sept 2019
• Date of issue
  Jul-Sep 2019

History

• Received
  7 Feb 2019
• Accepted
  19 Mar 2019
• Published
  7 Apr 2019