Trypanosoma cruzi: expression of antigenic component 5 among 35 laboratory clones obtained from 18 different isozymic variants

Breniere, Simone F.; Revollo, Susana; Caillard, Thierry; Velatte, Eric; Legrand, Dominique; Afchain, Daniel; Desjeux, Philippe

doi:10.1590/S0036-46651987000200003

Abstracts

Two monoclonal antibodies anti-component 5 of Trypanosoma cruzi (I-35/115 and II-190/30) were tested in IFA and ELISA respectively against 35 T. cruzi laboratory clones. Among the 35 clones tested, 18 different isozyme patterns were detected. All clones were recognized by both monoclonal antibodies except one clone which did not react with II-190/30. These results support the universal expression of specific component 5 within the taxon T. cruzi.

Trypanosoma cruzi; Antigenic components; Clones

Dois anticorpos monoclonais anticomponente 5 de Trypanosoma cruzi (I-35/115 e II-190/30) foram testados respectivamente em IFA e ELISA sobre 35 clones de T. cruzi isolados no laboratório. Entre estes 35 clones testados, 18 perfis isoenzímicos diferentes puderam ser detectados. Todos os clones foram reconhecidos exceto um clone que não reagiu com o anticor po monoclonal II-190/30. Estes resultados são a favor da expressão constante do componente 5 no seio do taxón T. cruzi.

ORIGINAL ARTICLES

Simone F. Breniere^I; Susana Revollo^II; Thierry Caillard^II; Eric Velatte^II; Dominique Legrand^III; Daniel Afchain^IV; Philippe Desjeux^V

^IORSTOM - IBBA, Casilla 8714, La Paz, Bolivia

^IIIBBA, c/o Embajada de Francia, Casilla 824, La Paz Bolivia

^IIIUniversite des Sciences et Techniques de Lille I, Laboratoire de Chimie Biologique associé au CNRS (LA 217) - IBBA. c/o Embajada de Francia, Casilla 824, La Paz, Bolivia

^IVC.I.B.P., Institut Pasteur de Lille, 15 rue Camille Guérin, 59019 Lille France

^VInstitut Pasteur de Paris - IBBA, c/o Embajada de Francia, Casilla 824, La Paz, Bolivia

SUMMARY

Two monoclonal antibodies anti-component 5 of Trypanosoma cruzi (I-35/115 and II-190/30) were tested in IFA and ELISA respectively against 35 T. cruzi laboratory clones. Among the 35 clones tested, 18 different isozyme patterns were detected. All clones were recognized by both monoclonal antibodies except one clone which did not react with II-190/30. These results support the universal expression of specific component 5 within the taxon T. cruzi.

Key words:Trypanosoma cruzi: Antigenic components; Clones.

RESUMO

Dois anticorpos monoclonais anticomponente 5 de Trypanosoma cruzi (I-35/115 e II-190/30) foram testados respectivamente em IFA e ELISA sobre 35 clones de T. cruzi isolados no laboratório. Entre estes 35 clones testados, 18 perfis isoenzímicos diferentes puderam ser detectados. Todos os clones foram reconhecidos exceto um clone que não reagiu com o anticor po monoclonal II-190/30. Estes resultados são a favor da expressão constante do componente 5 no seio do taxón T. cruzi.

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ACKNOWLEDGEMENTS

This work was supported by a grant from the French Ministry of Foreign Affairs (Cooperation & Development).

The authors wish to thank Dr. Michel Tibayrenc for giving the original strains of T. cruzi, and for its help in preparation of the manuscript.

Monoclonal antibodies were prepared and kindly given by Centre d'Immunologie et Biologie Parasitaire (Institut Pasteur of Lille, France).

REFERENCES

1. AFCHAIN, D.; LE RAY, D.; FRUIT, J. & CAPRON, A. - Antigenic make-up of Trypanosoma cruzi culture forms: identification of a specific component. J. Parasit., 65: 507-514, 1979.

2. ANTHONY, R. L.; JOHNSON, C M. & SOUSA, O. E. - Use of micro-ELISA for quantitating antibody to Trypanosoma cruzi and Trypanosoma rangeli. Amer. J. trop. Med. Hyg., 28: 969-973, 1979.

3. BRENIERE, S. F.; CARRASCO, R.; MIGUEZ, H.; LEMESRE, J. L. & CARLIER, Y. - Comparisons of immunological tests for serodiagnosis of Chagas'disease in Bolivian patients. Trop. geogr. Med., 37: 231-238, 1985.

4. BRENIERE, S. F.; CARRASCO, R.; MOLLINERO, S.; LEMESRE, J. L.; DESJEUX, P.; AFCHAIN, D. & CARLIER, Y. - Specific immunodiagnosis of Chagas' disease: immunodiffusion test using a specific serum anti Trypanosoma cruzi component 5. Trop. geogr. Med. (in press).

5. BRENIERE, S. F.; TIBAYRENC, M.; ANTEZANA, G.; PABON, J.; CARRASCO, R.; SELAES, H. & DESJEUX, P. - Résultats préliminaires en faveur d'une relation faible ou inexistante entre les formes cliniques de la maladie de Chagas et les souches isoenzymatiques de Trypanosoma cruzi. C. R. Acad. Sci. (Paris), 300: 555-557, 1985.

6. CHAPMAN, M. D.; SNARY, D. & MILES, M. A. - Quantitative differences in the expression of a 72,000 molecular weight cell surface glycoprotein (GP 72) in Trypanosoma cruzi zymodemes. J. Immunol., 132: 3149 3153, 1984.

7. DESJEUX, P.; LE PONT, F.; MOLLINEDO, S. & TIBAYRENC, M. - Les Leishmania de Bolivie. I: Leishmania braziliensis braziliensis dans les départements de La Paz et du Béni. Premiers isolements de souches humaines et caractérisation enzymatique. Ann. Parasit. hum. comp. (in press).

8. DVORAK, J. A. - The natural heterogeneity of Trypanosoma cruzi: biological and medical implications. J. cell. Biochem., 24: 357-371, 1984.

9. FRUIT, J.; AFCHAIN, D.; PETITPREZ, A. & CAPRON, A. - Trypanosoma cruzi: location of a specific antigen on the surface of bloodstream trypomastigote and culture epimastigote forms. Exp. Parasit., 45: 183-189, 1978.

10. GUIMARÃES, M. C; CELESTE, B. J.; AYRES, E. C; MINEO, J. R. & DINIZ, J. M. P. - Immunoenzymatic assay (ELISA) in mucocutaneous leishmaniasis, kalaazar and Chagas' disease: an epimastigote Trypanosoma cruzi antigen able to distinguish anti-Trypanosoma and anti Leishmania antibodies. Amer. J. trop. Med. Hyg., 30: 942-947, 1981.

11. KIRCHHOFF, L. V.; ENGEL, J. C; DVORAK, J. A. & SHER, A. - Strains and clones of Trypanosoma cruzi differ in their expression of a surface antigen identified by a monoclonal antibody. Mol. iBochem. Parasit., 11: 81-89, 1984.

12. LEMESRE, J. L.; AFCHAIN, D.; OROZCO, O.; LOYENS, M.: BRENIERE, S. P.; DESJEUX, P.; CARLIER, Y.; MARTIN, U.; NOGUEIRA-QUEIROZ, J. A.; LE RAY, D. & CAPRON, A. - Specific and sensitive immunological diagnosis of Chagas' disease by competitive antibody enzyme immunoassay using a Trypanosoma cruzi-specific monoclonal antibody. Amer. J. trop. Med. Hyg., 35: 86-93, 1986.

13. MILES, M. A.; DE SOUZA, A. A.; POVOA, M.; SHAW, J. J.; LAINSON, R. & TOYE, P. J. - Isozymic heterogeneity of Trypanosoma cruzi in the first autochthonous patients with Chagas' disease in Amazonian Brazil. Nature, 272: 819-821, 1978.

14. MILES, M. A.; TOYE, P. J.; OSWALDO, S. C. & GODFREY, D. G. - The identification by isoenzyme patterns of two distinct strain-groups of Trypanosoma cruzi, circulating independently in a rural area of Brazil. Trans, roy. Soc. trop. Med. Hyg., 71: 217-225, 1977.

15. OROZCO, O.; AFCHAIN, D.; DISSOUS, C; RODRIGUEZ, C; OLVAQUE, G.; LEMESRE, J. L.; LOYENS, M. & CAPRON, A. - Different monoclonal antibodies against the component 5 specific for Trypanosoma cruzi. Amer. J. trop. Med. Hyg., 33: 560-568, 1984.

16. READY, P. D. & MILES, M. A. - Delimitation ot Trypanosoma cruzi zymodemes by numerical taxonomy. Trans, roy. Soc. trop. Med. Hyg., 74: 238-242, 1980.

17. SCIIARFSTEIN, J.; RODRIGUEZ, M. M.; ALVES, C. A.; DE SOUZA, W.; PREVIATO, J. O. & PREVIATO, L- M. - Trypanosoma cruzi: description of a highly purified surface antigen defined by human antibodies. J. Immunol., 131: 972-978, 1983.

18. SCHECHTER, M.; LUQUETTI, A. O.; REZENDE, J„ M.; RASSI, A. & MILES, M. A. - Further evaluation of lectin affinity purified glycoprotein (GP 90) in the enzyme linked immunosorbent assay (ELISA) for diagnosis of Trypanosoma cruzi infection. Trans, roy. Soc. trop. Med. Hyg., 79: 637-640, 1985.

19. SCHECHTER, M.; STEVENS, A. F.; ALLEN, A. K. & MILES, M. A. - Investigations on the expression of a 72,000 molecular weight cell surface glycoprotein (GP 72) in Trypanosoma cruzi zymodemes and detection of anti-GP72 antibodies in chagasic patients. Trans, roy. Soc. trop. Med. Hyg., 79: 731, 1985.

20. SNARY, D.; FERGUSON, M. A. J.; SCOTT, M. T. & ALLEN, A. K. - Cell surface antigens of Trypanosoma cruzi: use of monoclonal antibodies to identify and isolate an epimastigote specific glycoprotein. Mol. Biochem. Parasit., 3: 343-356, 1981.

21. SNARY, D. & HUDSON, L. - Trypanosoma cruzi cell surface proteins: identification of one major glycoproten. Febs. Letters, 100: 166-170, 1979.

22. SOUZA, O. E. & JOHNSON, C. M. - Frequency and distribution of Trypanosoma cruzi and Trypanosoma rangeli in the Republic of Panama. Amer. J. trop. Med. Hyg., 20: 405-410, 1971.

23. TIBAYRENC, M.; ECHALAR, L.; DUJARDIN, J. P.; POCH, O. & DESJEUX, P. - The microdistribution of isoenzymic strains of Trypanosoma cruzi in Southern Bolovia; new isoenzyme profiles and further arguments against Mendelian sexuality. Trans, roy. Soc. trop. Med. Hyg., 78: 519-525, 1984.

24. TIBAYRENC, M. & LE RAY, D. - General classification of the isoenzymic strains of Trypanosoma (Scbizotrypanum) cruzi and comparison with T. (S.) c. marinkellei and T. (Herpetosoma) rangeli. Ann. Soc. belge Méd. trop., 64: 239-248, 1984.

25. TIBAYRENC, M.; WARD, P.; MOYA, A. & AYALA, F. J. - Natural populations of Trypanosoma cruzi, the agent of Chagas' disease, have a complex multiclonal structure. Proc. natl. Acad. Sci. (Wash.), 83: 115-119, 1986.

26. WHO. Parasite antigens. Bull. Wld. Hlth. Org., 52: 237-249, 1975.

27. WORLD HEALTH ORGANIZATION Report. News Letter, 18: 7, 1982.

Recebido para publicação em 27/6/86.

1. AFCHAIN, D.; LE RAY, D.; FRUIT, J. & CAPRON, A. - Antigenic make-up of Trypanosoma cruzi culture forms: identification of a specific component. J. Parasit., 65: 507-514, 1979.
2. ANTHONY, R. L.; JOHNSON, C M. & SOUSA, O. E. - Use of micro-ELISA for quantitating antibody to Trypanosoma cruzi and Trypanosoma rangeli Amer. J. trop. Med. Hyg., 28: 969-973, 1979.
3. BRENIERE, S. F.; CARRASCO, R.; MIGUEZ, H.; LEMESRE, J. L. & CARLIER, Y. - Comparisons of immunological tests for serodiagnosis of Chagas'disease in Bolivian patients. Trop. geogr. Med., 37: 231-238, 1985.
4. BRENIERE, S. F.; CARRASCO, R.; MOLLINERO, S.; LEMESRE, J. L.; DESJEUX, P.; AFCHAIN, D. & CARLIER, Y. - Specific immunodiagnosis of Chagas' disease: immunodiffusion test using a specific serum anti Trypanosoma cruzi component 5. Trop. geogr. Med. (in press).
5. BRENIERE, S. F.; TIBAYRENC, M.; ANTEZANA, G.; PABON, J.; CARRASCO, R.; SELAES, H. & DESJEUX, P. - Résultats préliminaires en faveur d'une relation faible ou inexistante entre les formes cliniques de la maladie de Chagas et les souches isoenzymatiques de Trypanosoma cruzi C. R. Acad. Sci. (Paris), 300: 555-557, 1985.
6. CHAPMAN, M. D.; SNARY, D. & MILES, M. A. - Quantitative differences in the expression of a 72,000 molecular weight cell surface glycoprotein (GP 72) in Trypanosoma cruzi zymodemes. J. Immunol., 132: 3149 3153, 1984.
7. DESJEUX, P.; LE PONT, F.; MOLLINEDO, S. & TIBAYRENC, M. - Les Leishmania de Bolivie. I: Leishmania braziliensis braziliensis dans les départements de La Paz et du Béni. Premiers isolements de souches humaines et caractérisation enzymatique. Ann. Parasit. hum. comp. (in press)
8. DVORAK, J. A. - The natural heterogeneity of Trypanosoma cruzi: biological and medical implications. J. cell. Biochem., 24: 357-371, 1984.
9. FRUIT, J.; AFCHAIN, D.; PETITPREZ, A. & CAPRON, A. - Trypanosoma cruzi: location of a specific antigen on the surface of bloodstream trypomastigote and culture epimastigote forms. Exp. Parasit., 45: 183-189, 1978.
10. GUIMARÃES, M. C; CELESTE, B. J.; AYRES, E. C; MINEO, J. R. & DINIZ, J. M. P. - Immunoenzymatic assay (ELISA) in mucocutaneous leishmaniasis, kalaazar and Chagas' disease: an epimastigote Trypanosoma cruzi antigen able to distinguish anti-Trypanosoma and anti Leishmania antibodies Amer. J. trop. Med. Hyg., 30: 942-947, 1981.
11. KIRCHHOFF, L. V.; ENGEL, J. C; DVORAK, J. A. & SHER, A. - Strains and clones of Trypanosoma cruzi differ in their expression of a surface antigen identified by a monoclonal antibody. Mol. iBochem. Parasit., 11: 81-89, 1984.
12. LEMESRE, J. L.; AFCHAIN, D.; OROZCO, O.; LOYENS, M.: BRENIERE, S. P.; DESJEUX, P.; CARLIER, Y.; MARTIN, U.; NOGUEIRA-QUEIROZ, J. A.; LE RAY, D. & CAPRON, A. - Specific and sensitive immunological diagnosis of Chagas' disease by competitive antibody enzyme immunoassay using a Trypanosoma cruzi-specific monoclonal antibody. Amer. J. trop. Med. Hyg., 35: 86-93, 1986.
13. MILES, M. A.; DE SOUZA, A. A.; POVOA, M.; SHAW, J. J.; LAINSON, R. & TOYE, P. J. - Isozymic heterogeneity of Trypanosoma cruzi in the first autochthonous patients with Chagas' disease in Amazonian Brazil. Nature, 272: 819-821, 1978.
14. MILES, M. A.; TOYE, P. J.; OSWALDO, S. C. & GODFREY, D. G. - The identification by isoenzyme patterns of two distinct strain-groups of Trypanosoma cruzi, circulating independently in a rural area of Brazil. Trans, roy. Soc. trop. Med. Hyg., 71: 217-225, 1977.
15. OROZCO, O.; AFCHAIN, D.; DISSOUS, C; RODRIGUEZ, C; OLVAQUE, G.; LEMESRE, J. L.; LOYENS, M. & CAPRON, A. - Different monoclonal antibodies against the component 5 specific for Trypanosoma cruzi Amer. J. trop. Med. Hyg., 33: 560-568, 1984.
16. READY, P. D. & MILES, M. A. - Delimitation ot Trypanosoma cruzi zymodemes by numerical taxonomy. Trans, roy. Soc. trop. Med. Hyg., 74: 238-242, 1980.
17. SCIIARFSTEIN, J.; RODRIGUEZ, M. M.; ALVES, C. A.; DE SOUZA, W.; PREVIATO, J. O. & PREVIATO, L- M. - Trypanosoma cruzi: description of a highly purified surface antigen defined by human antibodies. J. Immunol., 131: 972-978, 1983.
19. SCHECHTER, M.; STEVENS, A. F.; ALLEN, A. K. & MILES, M. A. - Investigations on the expression of a 72,000 molecular weight cell surface glycoprotein (GP 72) in Trypanosoma cruzi zymodemes and detection of anti-GP72 antibodies in chagasic patients. Trans, roy. Soc. trop. Med. Hyg., 79: 731, 1985.
20. SNARY, D.; FERGUSON, M. A. J.; SCOTT, M. T. & ALLEN, A. K. - Cell surface antigens of Trypanosoma cruzi: use of monoclonal antibodies to identify and isolate an epimastigote specific glycoprotein. Mol. Biochem. Parasit., 3: 343-356, 1981.
21. SNARY, D. & HUDSON, L. - Trypanosoma cruzi cell surface proteins: identification of one major glycoproten. Febs. Letters, 100: 166-170, 1979.
22. SOUZA, O. E. & JOHNSON, C. M. - Frequency and distribution of Trypanosoma cruzi and Trypanosoma rangeli in the Republic of Panama. Amer. J. trop. Med. Hyg., 20: 405-410, 1971.
23. TIBAYRENC, M.; ECHALAR, L.; DUJARDIN, J. P.; POCH, O. & DESJEUX, P. - The microdistribution of isoenzymic strains of Trypanosoma cruzi in Southern Bolovia; new isoenzyme profiles and further arguments against Mendelian sexuality. Trans, roy. Soc. trop. Med. Hyg., 78: 519-525, 1984.
24. TIBAYRENC, M. & LE RAY, D. - General classification of the isoenzymic strains of Trypanosoma (Scbizotrypanum) cruzi and comparison with T. (S.) c. marinkellei and T. (Herpetosoma) rangeli. Ann. Soc. belge Méd. trop., 64: 239-248, 1984.
25. TIBAYRENC, M.; WARD, P.; MOYA, A. & AYALA, F. J. - Natural populations of Trypanosoma cruzi, the agent of Chagas' disease, have a complex multiclonal structure. Proc. natl. Acad. Sci. (Wash.), 83: 115-119, 1986.
26. WHO. Parasite antigens. Bull. Wld. Hlth. Org., 52: 237-249, 1975.
27. WORLD HEALTH ORGANIZATION Report. News Letter, 18: 7, 1982.