NOTA
Sobrevivência de Rhizobium phaseoli em turfa esterilizada
Survival of Rhizobium phaseoli in sterilized peat
Eli S. Lopes; Antônio Roberto Giardini* * Com bolsa de suplementação do CNPq Os autores agradecem à firma TURFAL - Ind Com. Prod. Quim. Agron. Ltda., Sete Barras, PR
SUMMARY
Survival of R. phaseoli strains SMS-371 (=127 K-17, Nitragin Co. (USA) and SMS-376 (=CIAT-157, original CC-511) on peat was checked over a period of 240-250 days storage in the refrigerator.
The peat sample had been ground, limed (10% lime; final pH=7.0) and dried (80°C) for commercial use.
Both strains were cultivated on yeast manitol liquid media (pH=6.8) and mixed with the peat, when numbers of rhizobia were greater than 109 cells/ml, by direct counting.
Inoculant moisture was around 33%. Five plate counts were made in duplicate, during the storage period. In both strains a decrease in cell number was observed, in the 4th counting date (215-222 days storage), but even so rhizobia population was greater than 1.2 x 108 at the final sampling.
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Recebida para publicação em 22 de dezembro de 1976.
Datas de Publicação
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Publicação nesta coleção
19 Dez 2007 -
Data do Fascículo
1977
