Efeitos de herbicidas nos teores de clorofilas de Spirodela punctata

Santos, Durvalina M. M. dos; Pitelli, Robinson A.; Banzatto, David A.

doi:10.1590/S0100-83581999000200001

Resumos

Com o objetivo de verificar na planta aquática Spirodela punctata o efeito de diferentes concentrações (0,005; 0,05; 0,5 e 5 mgL-1 de ingrediente ativo) dos herbicidas butachlor, glyphosate e propanil sobre os teores de clorofila a, b e na razão clorofila a/b, foram conduzidos, por sete dias completos, bioensaios em sala climatizada. Determinou-se os teores de clorofilas através de leituras espectrofotométricas nas absorbâncias A645 e A663 nm. Os resultados mostraram que o butachlor diminuiu o teor de clorofila b, o glyphosate o teor de clorofila a, enquanto o propanil diminuiu os teores de clorofila a, b e a razão a/b, provocando a maior redução de pigmentos na espécie.

Pigmentos; butachlor; propanil; glyphosate; planta aquática

Effect of the herbicides on the chlorophyll content of Spirodela punctata The aim of this study was to verify the effects of the herbicides butachlor, glyphosate and propanil on content of chlorophyll of aquatic plant Spirodela punctata. In order to determine the effect of different concentrations of 0.005; 0.05; 0.5 and 5 mgL-1 (active ingredient) of three herbicides on the contents of chlorophyll a, b and a/b ratio. The bioassays were conducted, for a period of seven full days, in a climatedcontrolled room. Chlorophyll content was determined by spectrophotometric absorbency readings at A645 and A663 nm. Butachlor reduced chlorophyll b, the glyphosate decrease chlorophyll a, while the propanil reduced chlorophyll a, b and a/b ratio, causing the greatest reduction of the pigments in the species.

Pigments; butachlor; propanil; glyphosate; aquatic plant

Efeitos de herbicidas nos teores de clorofilas de Spirodela punctata¹ 1 Parte da tese do primeiro autor apresentada para obtenção do título de Doutor.

Effect of the herbicides on the chlorophyll content of Spirodela punctata

Durvalina M. M. dos Santos^I; Robinson A. Pitelli^II; David A. Banzatto^III

^IProf. Assistente Dr., Departamento de Biologia Aplicada Agropecuária, FCAV/UNESP. Rodovia Carlos Tonanni, km 5. Jaboticabal/SP. CEP: 14870-000

^IIProf. Titular, Departamento de Biologia Aplicada Agropecuária, FCAV/UNESP, Jaboticabal/SP

^IIIProf.Adjunto, Departamento de Ciências Exatas, FCAV/UNESP, Jaboticabal/SP

RESUMO

Com o objetivo de verificar na planta aquática Spirodela punctata o efeito de diferentes concentrações (0,005; 0,05; 0,5 e 5 mgL-1 de ingrediente ativo) dos herbicidas butachlor, glyphosate e propanil sobre os teores de clorofila a, b e na razão clorofila a/b, foram conduzidos, por sete dias completos, bioensaios em sala climatizada. Determinou-se os teores de clorofilas através de leituras espectrofotométricas nas absorbâncias A645 e A663 nm. Os resultados mostraram que o butachlor diminuiu o teor de clorofila b, o glyphosate o teor de clorofila a, enquanto o propanil diminuiu os teores de clorofila a, b e a razão a/b, provocando a maior redução de pigmentos na espécie.

Palavras chave: Pigmentos, butachlor, propanil, glyphosate, planta aquática.

ABSTRACT

Effect of the herbicides on the chlorophyll content of Spirodela punctata The aim of this study was to verify the effects of the herbicides butachlor, glyphosate and propanil on content of chlorophyll of aquatic plant Spirodela punctata. In order to determine the effect of different concentrations of 0.005; 0.05; 0.5 and 5 mgL-1 (active ingredient) of three herbicides on the contents of chlorophyll a, b and a/b ratio. The bioassays were conducted, for a period of seven full days, in a climatedcontrolled room. Chlorophyll content was determined by spectrophotometric absorbency readings at A645 and A663 nm. Butachlor reduced chlorophyll b, the glyphosate decrease chlorophyll a, while the propanil reduced chlorophyll a, b and a/b ratio, causing the greatest reduction of the pigments in the species.

Key words: Pigments, butachlor, propanil, glyphosate, aquatic plant.

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LITERATURA CITADA

Recebido para publicação em 29/01/97 e na forma revisada em 21/09/99.

ALMEIDA, F.S., RODRIGUES, B.N. Guia de herbicidas 2. ed., Londrina, Brasil. 1988. p. 131-136, 324-330, 445-450.
ANDERSON, I.C., ROBERTSON, D. Role of carotenoids in protecting chlorophyll from photoreduction. Plant Physiol., v.35, p.531-534. 1960.
ARNON, D.I. Cooper enzymes in isolated chloroplast polyphenoloxidase in Beta vulgaris Plant Physiol., v.24, n.1, p.1-15. 1949.
BANZATTO, D.A., KRONKA, S.N. Exp. agríc. 2ed. Jaboticabal:FUNEP, 1992. 247 p.
BEAUMONT, G., BASTIN, R., THERRIEN, H.P. Effets physiologiques de l'atrazine í doses sublíétales sur Lemna minor L. 1. Influence sur la croissance, la teneur en chlorophylle, en proteíne et en azote soluble et total. Nat. Can. , v.103, p.527-533. 1976.
CAMPBELL , W.F., EVANS, J.O., REED, S.C. Effects of glyphosate on chloroplast ultrastructure of quackgrass mesophyll cells. Weed Sci., n.24, p.22-25. 1976.
CHEN, P.C. Effects of herbicides on growth and photosynthesis of Anabaena CH2 e CH3. Proceedings of The National Science Council Republic of China , B. Life Sciences, v.10, n.3, p.151-156. 1986.
COWGILL, U.M., MILAZZO, D.P., LANDENBERGER, B.D. A comparison of the effect of triclopyr triethylamine salt on two species of duckweed (Lemna) examined for a 7 and 14 day test period. Water Res., v.23, p.617-623. 1989.
FISICHELLA, G., TROPEA, M., SAMBUCO, G. L'influenza del glifosato sul contenuto di clorofilla e di amminoacidi nelle foglie di alcune specie arboree. Nota II. Tecnica Agricola., v.36, n.4, p.335-342. 1984.
GOOD, N.E. Inhibitors of the Hill reaction. Plant Physiol., v.36, n.6, p.788-803. 1961.
GOODWIN, T.W. The changes in carotenoid and chlorophyll pigments in the leaves of deciduous trees during autumn necrosis. Biochem. J., v.68, n.3, p.503-511. 1958.
GRENIER, G., BEAUMONT, G. Effets physiologiques de l'atrazine íĄ doses sublíétales sur Lemna minor 7. Incorporation d'íĄcetate-1,2-( 14C ) dans les groupes de lipide et leurs acids gras. Physiol. Plant., v.57, p.477-484. 1983.
HOFSTRA, G., SWITZER, C.M. The phytotoxicity to propanil. Weed Sci., v.16, p.23-28. 1968.
HUTNER, S.H. Comparative physiology of heterotrophic growth. In: LOOMIS, W.E. (ed.), Growth and differentiation in plants Iowa State Coll. Press. 1953. p.417-446.
KITCHEN, L.M., WITT, W.W., RIECK, C.E. Inhibition of chlorophyll accumulation by glyphosate. Weed Sci., v.29, n.4, p.513-516. 1981.
LANDOLT, E., KANDELER, R. The family of Lemnaceae - a monographic study: phytochemistry, physiology, application, bibliography. Biosystematic investigation the family of duckweeds. Veroffenteichungendes Geobotanischen Institutes der Eidg. Techn. Hochschule, (ETH). 1987. v.4, n.95, 638 p.
LIU, A., TSAI, W.F. Effect of butachlor on rice and some paddy weeds. J. of the Agric. Assoc. of China, n.135, p.1-9. 1986.
LOCKHART, W.L., BILLECK, B.N., BARON, C.L. Bioassays with a floating aquatic plant (Lemna minor) for effects of sprayed and dissolved glyphosate. Hidrobiol., n.188/189, p.353-359. 1989.
MATTOO, A.K., WERGIN, W.P., ST. JOHN, J.B. Adaptative reorganization of thylakoid components of Spirodela oligorrhiza cultured on sublethal doses of atrazine. Plant Physiol., v.72, p.56, 1983. (Suplemento).
PANDY, A.K. Effects of propanil on growth and cell constituents of Nostoc calcicola Pestic. Biochem. and Physiol., v.23, n.2, p.157-162. 1985.
PIHAKASKI, S., PIHAKASKI, K. Effects of glyphosate on ultrastructure and photosynthesis of Pellia epiphylla Ann. Bot., v.46, p.133-141. 1980.
PRICE, D.N., WAIN, R.L. Studies on plant growth-regulating substances. XLI. Structure-activity relationships and metabolism of a group of nitrophenols capable of inhibiting chloroplast development. Ann. Appl. Biol., v.83, p.115-124. 1976.
RANDHAWA, S.K., GILL, H.S. Influence of herbicides applied in transplanted rice on chlorophyll and carotenoids in wheat leafs. J. Res., v.26, n.1, p.29-30. 1989.
SANDMANN, G., BOGER, P. Herbicides affecting plant pigments. British Crop Protection Conference Weeds. Proceedings ..., v.1, p.139-148. 1987.
SIMARD, S., PROTEAU, L., BEAUMONT, G., GRENIER, G. Increase of chloroplast granum stability, indiced by sublethal concentrations of atrazine, in Lemna minor fronds grown on a medium partially deficient in calcium or magnesium. Plant Physiol. Biochem.,v.29, p. 631-638. 1991.
TREICHEL, S. Einfluss von morphaktinen auf den stoffwechsel hoherer pflanzen. I. die wirkung auf die atmung und auf einige glykollytische enzyme. Biochem. Physiol. Pflanzen., v.166, p.481-493. 1974a.
TREICHEL, S. Einfluss von morphaktinen auf den stoffwechsel hoherer pflanzen. II. die wirkung auf die photosynthese und den gehalt an starke, ATP aun chlorophyll. Biochem. Physiol. Pflanzen., v.116, p.495-509. 1974b.
WANG, W. Uses of aquatic plants in ecotoxicology. Environ. Internat., v.10, p. i-iii, 1984. (Editorial).
WEJNAR, R., HAUSDORFER, R., SCHMIDT, M., WEISSENBORN, F. Investigations on photosynthetic pigments of Lemnaceae. 13. Comparative analysis of the bleaching effect of the herbicides amitrole, diuron and paraquat, with special consideration of NOEC. Angew. Bot., v.66, p.5-6. 1992.
WEJNAR, R., MICHEL, D. Untersuchungen zum einfluss des nitrofens auf die chloroplastenstruktur von Lemna gibba L. Beitr. Biol. Pflanzen.,v.58, p.435-441. 1983.
WEJNAR, R., TAIS, S. Untersuchungen zum einfluss des nitrofens auf wachstum und pigmentgehalt von Lemna gibba L. Beitr. Biol. Pflanzen., v.58, p.427-434. 1983.
WOLF, F.T. Changes in chlorophyll a and b in autumn leaves. Am. J. Bot., v.43, p.714-718. 1956.
WOLF, F.T. Effects of chemical agents in inhibition of chlorophyll synthesis and chloroplast development in higher plants. Bot. Ver., v.43, p.395-425. 1977.
YOGO, Y., ISHIZUKA, K. Tolerance of finger millet to propanil. 3. Relationship between propanil binding by chloroplast and inhibition of photosynthetic eletron transport. Weed Res., v.31, n.2, p.152-156. 1986.
ZIRSCHKY, J., REED, S.C. The use of duckweed for wastewater treatment. J. Water Poll. Control Fed., v.60, n.7, p.1253-1258, 1988.

1

Parte da tese do primeiro autor apresentada para obtenção do título de Doutor.

Datas de Publicação

Publicação nesta coleção
11 Jun 2010
Data do Fascículo
Ago 1999

Histórico

Aceito
21 Set 1999
Recebido
29 Jan 1997

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] ALMEIDA, F.S., RODRIGUES, B.N. Guia de herbicidas 2. ed., Londrina, Brasil. 1988. p. 131-136, 324-330, 445-450.

[2] ANDERSON, I.C., ROBERTSON, D. Role of carotenoids in protecting chlorophyll from photoreduction. Plant Physiol., v.35, p.531-534. 1960.

[3] ARNON, D.I. Cooper enzymes in isolated chloroplast polyphenoloxidase in Beta vulgaris Plant Physiol., v.24, n.1, p.1-15. 1949.

[4] BANZATTO, D.A., KRONKA, S.N. Exp. agríc. 2ed. Jaboticabal:FUNEP, 1992. 247 p.

[5] BEAUMONT, G., BASTIN, R., THERRIEN, H.P. Effets physiologiques de l'atrazine í doses sublíétales sur Lemna minor L. 1. Influence sur la croissance, la teneur en chlorophylle, en proteíne et en azote soluble et total. Nat. Can. , v.103, p.527-533. 1976.

[6] CAMPBELL , W.F., EVANS, J.O., REED, S.C. Effects of glyphosate on chloroplast ultrastructure of quackgrass mesophyll cells. Weed Sci., n.24, p.22-25. 1976.

[7] CHEN, P.C. Effects of herbicides on growth and photosynthesis of Anabaena CH2 e CH3. Proceedings of The National Science Council Republic of China , B. Life Sciences, v.10, n.3, p.151-156. 1986.

[8] COWGILL, U.M., MILAZZO, D.P., LANDENBERGER, B.D. A comparison of the effect of triclopyr triethylamine salt on two species of duckweed (Lemna) examined for a 7 and 14 day test period. Water Res., v.23, p.617-623. 1989.

[9] FISICHELLA, G., TROPEA, M., SAMBUCO, G. L'influenza del glifosato sul contenuto di clorofilla e di amminoacidi nelle foglie di alcune specie arboree. Nota II. Tecnica Agricola., v.36, n.4, p.335-342. 1984.

[10] GOOD, N.E. Inhibitors of the Hill reaction. Plant Physiol., v.36, n.6, p.788-803. 1961.

[11] GOODWIN, T.W. The changes in carotenoid and chlorophyll pigments in the leaves of deciduous trees during autumn necrosis. Biochem. J., v.68, n.3, p.503-511. 1958.

[12] GRENIER, G., BEAUMONT, G. Effets physiologiques de l'atrazine íĄ doses sublíétales sur Lemna minor 7. Incorporation d'íĄcetate-1,2-( 14C ) dans les groupes de lipide et leurs acids gras. Physiol. Plant., v.57, p.477-484. 1983.

[13] HOFSTRA, G., SWITZER, C.M. The phytotoxicity to propanil. Weed Sci., v.16, p.23-28. 1968.

[14] HUTNER, S.H. Comparative physiology of heterotrophic growth. In: LOOMIS, W.E. (ed.), Growth and differentiation in plants Iowa State Coll. Press. 1953. p.417-446.

[15] KITCHEN, L.M., WITT, W.W., RIECK, C.E. Inhibition of chlorophyll accumulation by glyphosate. Weed Sci., v.29, n.4, p.513-516. 1981.

[16] LANDOLT, E., KANDELER, R. The family of Lemnaceae - a monographic study: phytochemistry, physiology, application, bibliography. Biosystematic investigation the family of duckweeds. Veroffenteichungendes Geobotanischen Institutes der Eidg. Techn. Hochschule, (ETH). 1987. v.4, n.95, 638 p.

[17] LIU, A., TSAI, W.F. Effect of butachlor on rice and some paddy weeds. J. of the Agric. Assoc. of China, n.135, p.1-9. 1986.

[18] LOCKHART, W.L., BILLECK, B.N., BARON, C.L. Bioassays with a floating aquatic plant (Lemna minor) for effects of sprayed and dissolved glyphosate. Hidrobiol., n.188/189, p.353-359. 1989.

[19] MATTOO, A.K., WERGIN, W.P., ST. JOHN, J.B. Adaptative reorganization of thylakoid components of Spirodela oligorrhiza cultured on sublethal doses of atrazine. Plant Physiol., v.72, p.56, 1983. (Suplemento).

[20] PANDY, A.K. Effects of propanil on growth and cell constituents of Nostoc calcicola Pestic. Biochem. and Physiol., v.23, n.2, p.157-162. 1985.

[21] PIHAKASKI, S., PIHAKASKI, K. Effects of glyphosate on ultrastructure and photosynthesis of Pellia epiphylla Ann. Bot., v.46, p.133-141. 1980.

[22] PRICE, D.N., WAIN, R.L. Studies on plant growth-regulating substances. XLI. Structure-activity relationships and metabolism of a group of nitrophenols capable of inhibiting chloroplast development. Ann. Appl. Biol., v.83, p.115-124. 1976.

[23] RANDHAWA, S.K., GILL, H.S. Influence of herbicides applied in transplanted rice on chlorophyll and carotenoids in wheat leafs. J. Res., v.26, n.1, p.29-30. 1989.

[24] SANDMANN, G., BOGER, P. Herbicides affecting plant pigments. British Crop Protection Conference Weeds. Proceedings ..., v.1, p.139-148. 1987.

[25] SIMARD, S., PROTEAU, L., BEAUMONT, G., GRENIER, G. Increase of chloroplast granum stability, indiced by sublethal concentrations of atrazine, in Lemna minor fronds grown on a medium partially deficient in calcium or magnesium. Plant Physiol. Biochem.,v.29, p. 631-638. 1991.

[26] TREICHEL, S. Einfluss von morphaktinen auf den stoffwechsel hoherer pflanzen. I. die wirkung auf die atmung und auf einige glykollytische enzyme. Biochem. Physiol. Pflanzen., v.166, p.481-493. 1974a.

[27] TREICHEL, S. Einfluss von morphaktinen auf den stoffwechsel hoherer pflanzen. II. die wirkung auf die photosynthese und den gehalt an starke, ATP aun chlorophyll. Biochem. Physiol. Pflanzen., v.116, p.495-509. 1974b.

[28] WANG, W. Uses of aquatic plants in ecotoxicology. Environ. Internat., v.10, p. i-iii, 1984. (Editorial).

[29] WEJNAR, R., HAUSDORFER, R., SCHMIDT, M., WEISSENBORN, F. Investigations on photosynthetic pigments of Lemnaceae. 13. Comparative analysis of the bleaching effect of the herbicides amitrole, diuron and paraquat, with special consideration of NOEC. Angew. Bot., v.66, p.5-6. 1992.

[30] WEJNAR, R., MICHEL, D. Untersuchungen zum einfluss des nitrofens auf die chloroplastenstruktur von Lemna gibba L. Beitr. Biol. Pflanzen.,v.58, p.435-441. 1983.

[31] WEJNAR, R., TAIS, S. Untersuchungen zum einfluss des nitrofens auf wachstum und pigmentgehalt von Lemna gibba L. Beitr. Biol. Pflanzen., v.58, p.427-434. 1983.

[32] WOLF, F.T. Changes in chlorophyll a and b in autumn leaves. Am. J. Bot., v.43, p.714-718. 1956.

[33] WOLF, F.T. Effects of chemical agents in inhibition of chlorophyll synthesis and chloroplast development in higher plants. Bot. Ver., v.43, p.395-425. 1977.

[34] YOGO, Y., ISHIZUKA, K. Tolerance of finger millet to propanil. 3. Relationship between propanil binding by chloroplast and inhibition of photosynthetic eletron transport. Weed Res., v.31, n.2, p.152-156. 1986.

[35] ZIRSCHKY, J., REED, S.C. The use of duckweed for wastewater treatment. J. Water Poll. Control Fed., v.60, n.7, p.1253-1258, 1988.

Brasil

Brasil

Efeitos de herbicidas nos teores de clorofilas de Spirodela punctata

Effect of the herbicides on the chlorophyll content of Spirodela punctata

Resumos

Datas de Publicação

Histórico