Bacterial wilt, caused by Curtobacterium flaccumfaciens pv. flaccumfaciens, is an important disease for bean (Phaseolus vulgaris L.) production in Southern Brazil. The use of contamined seeds is the major way to spread the bacterium. Although the occurrence of this disease in Brazil is recent, the seedborne bacterium hase been disseminated to most of the bean producing areas. The objective of this study was to adjust the CNS (Corynebacterium Nebraskense Selective Medium) to recover and detect the bacterium in infected soil as well as in bean seeds. Suspensions obtained from washing infected soil and bean seeds contamined by the bacterium were plated onto modified CNS medium. The modified CNS was efficient to recover the bacterium from both contaminated soil and bean seeds. The CNS established medium differed from the original by reducing the concentration of polymyxin B sulfate to 16 mg/l, excluding cyclohexamide and replacing Daconil 2787-F fungicide (530 mg/ml chlorothalonil) with Dacostar 500 (500 mg/ml chlorothalonil).
Phaseolus vulgaris; bacterial wilt; seed pathology; survival
