Cellulolytic complex produced by two species of phytopathogenic fungi isolated from cassava

Morais, Martival dos Santos; Oliveira, Neiva Tinti de; Herculano, Polyanna Nunes; Moreira, Keila Aparecida

doi:10.1590/0100-5405/2151

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ARTIGOS • Summa Phytopathol. 42 (3) • Jul-Sep 2016 • https://doi.org/10.1590/0100-5405/2151 copy

Cellulolytic complex produced by two species of phytopathogenic fungi isolated from cassava

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Material collected from commercial crops of cassava (Manihot esculenta Crantz) in 11 municipalities in the state of Paraíba included 27 isolates of the fungi Colletotrichum gloeosporoides f. sp. manihotis Henn (Penn.) and Fusarium oxysporum Schlent., which are pathogenic to the culture. From these isolates, cellulolytic enzyme complex production was obtained under submerged fermentation at 120 rpm and 30 °C for 7 days, with CMC (Carboxymethyl Cellulose) as carbon source. Enzyme activities were quantified by means of the degree of hydrolysis of strips of filter paper (FPAse) and CMC (CMCase). The release of Paranitrofenol was used to quantify the activities of β-glucosidase (βG). The highest activities were: 23.13 and 1.51 U/ml for FPase and CMCase by the isolates of C. gloeosporioides URM 7124 and 7125. For βG, 0.0014 U/ml by isolate 13.

Keywords
Fusarium oxysporum; Colletotrichum gloeosporioides; FPase; Endoglucanase; enzymes

Isolado	Espécie	Município	Localização
1	Colletotrichum gloeosporioides	Lagoa Nova I	S: 07°03’12”/O: 35°45’55”
2	Colletotrichum gloeosporioides	Lagoa Nova II	S: 07°03’14”/O: 35°45’56”
3	Colletotrichum gloeosporioides	S.Miguel Taipú II	S: 07°04’51”/O: 35°43’42”
4	Fusarium oxysporum	Imaculada II	S: 07°21’52”/O: 37°29’21”
5	Colletotrichum gloeosporioides	Matinhas II	S: 07°06’18”/O: 35°43’43”
6	Colletotrichum gloeosporioides	Pilões I	S: 06°51’56”/O: 35°37’47”
7	Colletotrichum gloeosporioides URM 7081	Pocinhos II	S: 07°08’35”/O: 36°02’97”
8	Fusarium oxysporum URM 7083	Imaculada II	S: 07°21’52”/O: 37°29’21”
9	Colletotrichum gloeosporioides	Sapé I	S: 07°05’11”/O: 35°14’49”
10	Colletotrichum gloeosporioides	Cuité II	S: 06°27’98”/O: 36°10’27”
11	Colletotrichum gloeosporioides	Princesa Isabel I	S: 07°45’16”/O: 37°57’42”
12	Colletotrichum gloeosporioides	Pilões II	S: 06°51’53”/O: 35°37’48”
13	Colletotrichum gloeosporioides	Pocinhos I	S: 07°07’22”/O: 36°02’93”
14	Colletotrichum gloeosporioides	Teixeira II	S: 07°16’78”/O: 37°19’51”
15	Fusarium oxysporum	Princesa Isabel I	S: 07°44’86”/O: 37°57’23”
16	Colletotrichum gloeosporioides	Pocinhos I	S: 07°07’22”/O: 36°02’93”
17	Fusarium oxysporum	Princesa Isabel I	S: 07°44’86”/O: 37°57’23”
18	Colletotrchum gloeosporioides	Matinhas I	S: 07°06’17”/O: 35°48’42”
19	Fusarium oxysporum	Princesa Isabel II	S: 07°44’86”/O: 37°57’23”
20	Colletotrichum gloeosporioides	Cuité I	S: 06°27’96”/O: 36°10’13”
21	Colletotrichum gloeosporioides	Nova Floresta II	S: 06°27’12”/O: 36°13’43”
22	Colletotrichum gloeosporioides URM 7125	Areia I	S: 07°01’41”/O: 35°45’39”
23	Colletotrichum gloeosporioides URM 7124	Areia II	S: 07°02’65”/O: 35°37’73”
24	Fusarium oxysporum URM 7082	Imaculada I	S: 07°21’56”/O: 37°29’37”
25	Fusarium oxysporum	Imaculada I	S: 07°21’56”/O: 37°29’37”
26	Colletotrichum gloeosporioides URM 7080	Nova Floresta I	S: 06°27’11”/O: 36°13’42”
27	Colletotrichum gloeosporioides	Pocinhos II	S: 07°08’36”/O: 36°02’92”

Isolados	Espécie	FPAse	CMCase	bG
1	C. gloeosporioides	3.01c^* * médias seguidas da mesma letra não diferem entre si pelo teste de Scott-Knott a 5% de probabilidade	0.34c	0.0005c
2	C. gloeosporioides	3.71c	0.36c	0.0006c
3	C. gloeosporioides	1.27c	0.28d	0.0008b
4	F. oxysporum	3.85c	0.35c	0.0005c
5	C. gloeosporioides	2.84c	0.28d	0.0006c
6	C. gloeosporioides	3.02c	0.33c	0.0008b
7	C. gloeosporioides URM 7081	2.96c	0.24d	0.0013a
8	F. oxysporum URM 7083	2.85c	0.28d	0.0002e
9	C. gloeosporioides	4.73c	0.30d	0.0003d
10	C. gloeosporioides	4.96c	0.31d	0.0008c
11	C. gloeosporioides	13.21b	0.45c	0.0002e
12	C. gloeosporioides	5.12c	0.24d	0.0013a
13	C. gloeosporioides	10.95b	0.23d	0.0014a
14	C. gloeosporioides	0.95c	0.28d	0.0012a
15	F. oxysporum	5.22c	0.41c	0.0003d
16	C. gloeosporioides	4.69c	0.26d	0.0005c
17	F. oxysporum	3.69c	0.25d	0.0010b
18	C. gloeosporioides	1.08c	0.24d	0.0003d
19	F. oxysporum	4.92c	0.29d	0.0006c
20	C. gloeosporioides	1.01c	0.29d	0.0004c
21	C. gloeosporioides URM 7080	5.66c	0.24d	0.0004c
22	C. gloeosporioides URM 7125	4.29c	1.51a	0.0001e
23	C. gloeosporioides URM 7124	23.13a	1.20b	0.0001e
24	F. oxysporum URM 7082	5.89c	0.23d	0.0007c
25	F. oxysporum	4.79c	0.28d	0.0002e
26	C. gloeosporioides	5.55c	0.37c	0.0002e
27	C. gloeosporioides	5.51c	0.27d	0.0002e

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[1] Martival dos Santos Morais (martivalcg@hotmail.com).