ABSTRACT
Oil spot or bacterial spot of passion fruit, caused by the bacterium Xanthomonas campestris pv. passiflorae, is one of the most important diseases affecting this crop, leading to direct damage to fruit production and quality. The use of pathogen-free seeds is the major form of disease control, requiring bacterial detection in the seeds to prevent introduction into exempt areas. The objective of the present study was to detect the presence of X. campestris pv. passiflorae in passion fruit seeds in culture medium. The extract of whole or broken seeds, disinfected or not, from two passion fruit genotypes from Uberlândia, Minas Gerais State (MG), Brazil, were cultivated in the culture media 523 and NAS without and with the addition of cycloheximide. There was no significant difference between culture media for bacterial growth. Culture media 523 and NAS with the addition of cycloheximide and the use of broken passion fruit seeds were efficient at detecting X. campestris pv. passiflorae, which can be used in laboratory routine for detection of this bacterium in seeds.
Keywords
Bacterial spot; oil spot; culture medium;
Passiflora edulis
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