Polyacrylamide gel electrophoresis and combined immunoenzyme assay for rotavirus and adenovirus were used to analyze 380 fecal samples from children up to three years of age who were hospitalized with acute diarrhea in Campo Grande, State of Mato Grosso do Sul, between May 2000 and January 2004. Among all the samples, 88 (23. 2%) were positive for Rotavirus A. Out of these, 81 (92%) had a defined electrophoretic pattern: 77 (87. 5%) with a long pattern and four (4. 5%) with a short pattern. Genotype G and P characterization was done by nested RT-PCR for 85 samples, of which 56 (65. 9%) were genotyped as type G. Among these, 49 (87. 5%) were G1, five (8. 9%) were G4, one (1. 8%) was G3 and one (1. 8%) was G9. The genotype was found to be type P in 37 samples (43. 5%) and all of these were P[8]. The G and P association most observed was G1P[8], with 33 samples (89. 2%), followed by G4P[8], two samples (5. 4%); G3P[8], one sample (2. 7%); and G9P[8], one sample (2. 7%).
Rotavirus A; Infection; Children; Genotyping
