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Short Communication • Rev. Soc. Bras. Med. Trop. 52 • 2019 • https://doi.org/10.1590/0037-8682-0135-2019   copy

Larvicidal and adulticidal effects and ultrastructural changes of larvae midgut epithelium of Musca domestica (Diptera: Muscidae) fed with Bacillus thuringiensis var. kyushuensis

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative.

METHODS:

We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut.

RESULTS:

We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected.

CONCLUSIONS:

The serovar, Btk176 might be a potential biocontrol agent for houseflies.

Keywords:
Biological control, housefly; Entomopathogenic bacteria; Bacillus thuringiensis var. kyushuensis

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