Emergence of <i>aph(3’)-VI</i> and accumulation of aminoglycoside modifying enzyme genes in KPC-2-possessing <i>Enterobacter aerogenes</i> isolates from infections and colonization in patients from Recife-PE, Brazil

Firmo, Elza Ferreira; Cabral, Adriane Borges; Oliveira, Érica Maria de; Lopes, Ana Catarina Souza

doi:10.1590/0037-8682-0460-2018

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Short Communication • Rev. Soc. Bras. Med. Trop. 52 • 2019 • https://doi.org/10.1590/0037-8682-0460-2018 copy

Emergence of aph(3’)-VI and accumulation of aminoglycoside modifying enzyme genes in KPC-2-possessing Enterobacter aerogenes isolates from infections and colonization in patients from Recife-PE, Brazil

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

The objective of this study was to characterize genes of aminoglycoside modifying enzymes (AMEs) in colonizing and infecting isolates of E. aerogenes harboring bla _KPC from patients at a public hospital in Recife-PE, Brazil.

METHODS:

We analyzed 29 E. aerogenes clinical isolates resistant to aminoglycosides. AMEs genes were investigated by PCR and sequencing.

RESULTS:

Colonizing and infecting isolates mainly presented the genetic profiles aac(3)-IIa/aph(3’)-VI or ant(2”)-IIa/aph(3’)-VI. This is the first report of aph(3’)-VI in E. aerogenes harboring bla _KPC in Brazil.

CONCLUSIONS:

The results highlight the importance in establishing rigorous methods for the surveillance of resistance genes, especially in colonized patients.

Keywords:
Enterobacter aerogenes; Aminoglycoside modifying enzymes; Antimicrobial resistance; KPC

Isolates	Inpatient unit	Source of isolation	Resistance phenotypes	AMEs genes
			to aminoglycosides
Ea2A	UCO1	Rectal swab	III	aac(3)-IIa/aph(3’)-VI
Ea3A	UCO2	Rectal swab	I	aph(3’)-VI
Ea4A	CARDIO	Rectal swab	III	ant(2”)-IIa/aph(3’)-VI
Ea5A	ICU	Rectal swab	III	aac(3)-IIa/aph(3’)-VI
Ea6A	ICU	Rectal swab	III	aac(3)-IIa/aph(3’)-VI
Ea8A	ICU	Rectal swab	II	aac(3)-IIa/aph(3’)-VI
Ea13A	UCO1	Rectal swab	I	aph(3’)-VI
Ea14A	UCO1	Rectal swab	II	aac(3)-IIa/aph(3’)-VI
Ea16A	UCO1	Rectal swab	III	aph(3’)-VI
Ea17A	UCO1	Rectal swab	III	aac(3)-IIa
Ea20A	UCO1	Rectal swab	III	aac(3)-IIa/aph(3’)-VI
Ea23A	UCO1	Rectal swab	I	ant(2”)-Ia/aph(3’)-VI
Ea24A	ICU	Rectal swab	III	aac(3)-IIa/ant(2”)-Ia/aph(3’)-VI
Ea29A	UCO1	Rectal swab	I	ant(2”)-Ia/aph(3’)-VI
Ea31A	CARDIO	Rectal swab	III	aac(3)-IIa/aph(3’)-VI
Ea7A	ICU	Blood	II	aac(3)-IIa/aph(3’)-VI
Ea18A	ICU	Blood	III	ant(2”)-Ia/aph(3’)-VI
Ea27A	UCO1	Blood	III	aac(3)-IIa/aph(3’)-VI
Ea28A	ICU	Blood	I	ant(2”)-Ia/aph(3’)-VI
Ea32A	UCO1	Blood	I	ant(2”)-Ia/aph(3’)-VI
Ea33A	ICU	Blood	I	ant(2”)-Ia/aph(3’)-VI
Ea34A	ICU	Blood	I	ant(2”)-Ia
Ea10A	CARDIO	Urine	I	aph(3’)-VI
Ea11A	M. CLINIC	Urine	III	aac(3)-IIa/aph(3’)-VI
Ea26A	CARDIO	Urine	III	aac(3)-IIa/ant(2”)-Ia/aph(3’)-VI
Ea12A	ICU	Tip catheter	III	aac(3)-IIa/aph(3’)-VI
Ea15A	ICU	Tracheal aspirates	II	aac(3)-IIa
Ea19A	ICU	Tracheal aspirates	I	ant(2”)-Ia/aph(3’)-VI
Ea30A	ICU	Ascitic fluid	I	ant(2”)-Ia/aph(3’)-VI

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[1] Corresponding author: Profª Drª Ana Catarina de Souza Lopes. e-mail:ana.lopes.ufpe@gmail.com