Molecular epidemiology and drug resistance of <i>Acinetobacter baumannii</i> isolated from a regional hospital in the Brazilian Amazon region

Ribeiro, Edlainny Araujo; Gales, Ana Cristina; Oliveira, Ana Paula Streling de; Coelho, Danilo Dias; Oliveira, Rodrigo Alves de; Pfrimer, Irmtraut Araci Hoffmann; Carmo Filho, José Rodrigues do

doi:10.1590/0037-8682-0087-2020

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Short Communication • Rev. Soc. Bras. Med. Trop. 54 • 2021 • https://doi.org/10.1590/0037-8682-0087-2020 copy

Molecular epidemiology and drug resistance of Acinetobacter baumannii isolated from a regional hospital in the Brazilian Amazon region

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

In this study, we report a clonal dissemination of carbapenem resistant Acinetobacter baumannii isolates due to the acquisition of bla_OXA-23 in a regional hospital located in Brazilian Amazon Region.

METHODS:

The isolates were identified by MALDI-TOF and the carbapenemase-encoding genes were detected by multiplex-PCR. The genetic similarity was investigated by pulsed-field gel electrophoresis (PFGE).

RESULTS:

Only 10 (55.6%) isolates harbored the gene bla _OXA-23. PFGE analysis revealed that these isolates belong to a single clone.

CONCLUSIONS:

This dissemination strategy indicates the need for surveillance, adoption of control procedures defined in guidelines, and the careful administration of antimicrobials should be reinforced.

Keywords:
Acinetobacter baumannii; Carbapenem resistance; Carbapenemase; bla _OXA-23 ; Gram-negative bacilli

Isolates	MIC (µg/mL)				DISK DIFFUSION (mm)
	POL	TIG	MER	IMP	PIT	CPM	TET	CAZ	CTX	CIP	GEN	AMI	ATM
C1	2	≤ 2	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
C2	2	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
C3	< 0.25	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C4 *	1	≤ 2	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
C5	0.50	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
C6	0.50	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
C7	0.50	< 0.5	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C8**	1	≥ 8	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C9**	< 0.25	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
C10	0.50	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C11*	2	≥ 8	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C12**	NT	NT	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C13**	0.50	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C14*	2	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
C15	0.50	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C16**	0.50	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C17**	< 0.25	≥ 8	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R
→C18**	1	< 1	≥ 32	≥ 32	R	R	R	R	R	R	R	R	R

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[1] Corresponding author: Prof. Dr. José Rodrigues do Carmo Filho. e-mail:biomedico53@gmail.com