Invitro evaluation of hydrolytic enzyme activity and biofilm formation of Candida parapsilosis species complex from a nosocomial environment

Paula-Mattiello, Shaiana; Oliveira, Sílvia Dias de; Medina-Silva, Renata

doi:10.1590/0037-8682-0032-2017

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Revista da Sociedade Brasileira de Medicina Tropical

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Short Communications • Rev. Soc. Bras. Med. Trop. 50 (04) • Jul-Aug 2017 • https://doi.org/10.1590/0037-8682-0032-2017 copy

Invitro evaluation of hydrolytic enzyme activity and biofilm formation of Candida parapsilosis species complex from a nosocomial environment

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

INTRODUCTION:

Candidaparapsilosis complex species, frequently found in hospital environments, have gained importance as etiological agents of candidemia.

METHODS:

Candidaparapsilosis complex isolates from a nosocomial environment were identified and their hydrolitic enzyme activity and ability to form biofilm were characterized.

RESULTS:

Twenty-two C. parapsilosis sensu stricto isolates produced proteinase and three produced phospholipase. Most Candida metapsilosis isolates produced proteinase and one also produced phospholipase. All 29 isolates formed biofilms.

CONCLUSIONS:

The nosocomial environment may act as a reservoir for C. parapsilosis complex isolates with phenotypic features that could possibly lead to nosocomial infections and health complications in hospital patients.

Keywords:
Candida parapsilosis complex; Biofilm; Hydrolytic enzymes

Isolates	Origin	Proteinase activity (Pz)	Rating	Phospholipase activity (Pz)	Rating
CP.01	Chair 1	0.40	+ + + +	1.0	-
CP.02	Chair 2	0.31	+ + + +	1.0	-
CP.03	Bed 4	0.46	+ + + +	0.55	+ + + +
CM.04	Medication trolley 2	0.54	+ + + +	1.0	-
CP.05	Sphygmomanometer 2	0.47	+ + + +	1.0	-
CP.06	Sphygmomanometer 3	0.36	+ + + +	1.0	-
CP.07	Glove 1	0.35	+ + + +	0.52	+ + + +
CP.08	Room table 1	0.55	+ + + +	1.0	-
CM.09	Sphygmomanometer 4	1.0	-	1.0	-
CP.10	Room table 2	0.28	+ + + +	0.60	+ + + +
CP.11	Medication table 1	0.39	+ + + +	1.0	-
CP.12	Sphygmomanometer 1	0.43	+ + + +	1.0	-
CP.13	Glove 2	0.42	+ + + +	1.0	-
CP.14	Wheelchair 1	0.38	+ + + +	1.0	-
CP.15	Bed 5	0.52	+ + + +	1.0	-
CP.16	Sphygmomanometer 4	0.47	+ + + +	1.0	-
CP.17	Feed table 1	0.40	+ + + +	1.0	-
CP.18	Medication trolley 1	0.37	+ + + +	1.0	-
CM.19	Sphygmomanometer 3	0.57	+ + + +	1.0	-
CP.20	Feed table 2	0.40	+ + + +	1.0	-
CP.21	Medication trolley 2	0.40	+ + + +	1.0	-
CP.22	Medication trolley 3	0.47	+ + + +	1.0	-
CM.23	Medication trolley 4	0.55	+ + + +	1.0	-
CP.24	Medication trolley 5	0.36	+ + + +	1.0	-
CM.25	Sphygmomanometer 5	0.38	+ + + +	1.0	-
CP.26	Feed table 3	0.37	+ + + +	1.0	-
CM.27	Bed 1	1.0	-	1.0	-
CM.28	Feed table 1	0.60	+ + + +	0.42	+ + + +
CP.29	Feed table 4	0.36	+ + + +	1.0	-
C. parapsilosis ATCC 22019	-	1.0	-	1.0	-
C. albicans ATCC 18804	-	0.95	+	0.87	++

Sociedade Brasileira de Medicina Tropical - SBMT Caixa Postal 118, 38001-970 Uberaba MG Brazil, Tel.: +55 34 3318-5255 / +55 34 3318-5636/ +55 34 3318-5287, http://rsbmt.org.br/ - Uberaba - MG - Brazil
E-mail: rsbmt@uftm.edu.br

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[1] Corresponding author: Msc. Shaiana Paula-Mattiello. e-mail:shaiana_m@hotmail.com