Association between hematological profile and serum 25-hydroxyvitamin D levels and FokI polymorphism in individuals with cystic fibrosis

ASSIS, Milena Luana Coelho de; CARTAXO, Constantino Giovanni Braga; COSTA, Maria José de Carvalho; QUEIROZ, Dayanna Joyce Marques; PERSUHN, Darlene Carmati; GONÇALVES, Maria da Conceição Rodrigues

doi:10.1590/1678-98652018000200007

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ORIGINAL ARTICLES • Rev Nutr 31 (2) • Mar-Apr 2018 • https://doi.org/10.1590/1678-98652018000200007 copy

Association between hematological profile and serum 25-hydroxyvitamin D levels and FokI polymorphism in individuals with cystic fibrosis

Associação do perfil hematológico com os valores séricos de 25-hidroxivitamina D e polimorfismo FokI em indivíduos com fibrose cística

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Objective

The present study aimed at investigating the association between hematological profile and serum 25-hydroxyvitamin D (25[OH]D) levels and Fokl polymorphism of the vitamin D receptor gene in individuals with Cystic Fibrosis.

Methods

A cross-sectional study that involved 18 men and women aged 0-25 years with Cystic Fibrosis. Socio-demographic information and the factors associated with sun exposure were obtained. Weight, height, and arm circumference were also measured. Blood sample was collected for the analysis of biochemical parameters (25[OH]D, parathyroid hormone, and calcium levels and blood count) and for the validation of the presence of FokI polymorphism in the vitamin D receptor gene.

Results

Among the participants, 33.33% (n=6) had vitamin D deficiency (19.60±6.180 ng/mL), and 27.8% (n=5) presented with anemia and low weight for age. In terms of genotype, 5.6% (n=1) presented with the FF genotype, 72.3% (n=13) had the Ff genotype, and 22.2% (n=4) had the ff genotype. Serum 25(OH)D levels were associated with hemoglobin (p=0.008) and hematocrit (p=0.019) levels and leukocyte count (p=0.0114). No association was observed between 25(OH)D levels and the genotypes (FF, Ff, and ff) (p=0.2451). In addition, an association was observed between FokI polymorphism and the total leukocyte count (p=0.01).

Conclusion

An association was observed between serum 25(OH)D levels and hemoglobin and hematocrit levels and leukocyte count in individuals with Cystic Fibrosis. Moreover, FokI polymorphism was associated with total leukocyte count.

Keywords
Cystic fibrosis; Hemoglobins; Polymorphism; genetic; Vitamin D

Variables		Participants (n=18)		Individuals with normal 25(OH) D^* * 25(OH)D ≥75nmoL/L (30ng/mL). levels (n=12)		Individuals with low 25(OH) D^ 25(OH)D<75nmoL/L (30ng/mL). levels (n=6)
Variables		Prevalence	n	Prevalence	n	Prevalence	n
Gender
Male		55.6%0	10	75%	9	16.67%	1
Female		44.4% 0	8	25%	3	83.33%	5
Level of education
Illiterate		-	-	-	-	-	-
1st degree		44.45%	8	58.33%	7	16.67%	1
2nd degree		44.45%	8	41.67%	5	50%	3
Graduate		11.10%	2	-	-	33.33%	2
Post-graduate		-	-	-	-	-	-
Family income
1–5 minimum wages		88.88%	16	100%	12	66.66%	4
5–10 minimum wages		0 5.56%	1	-	-	16.67%	1
>10 minimum wages		0 5.56%	1	-	-	16.67%	1
Skin color
Very pale		16.67%	3	-	-	50%	3
Fair		22.22%	4	16.67%	2	33.33%	2
Less fair		16.67%	3	16.67%	2	16.67%	1
Light brown		16.67%	3	25%	3	-	-
Dark brown		22.22%	4	33.33%	4	-	-
Black		0 5.55%	1	0 8.33%	1	-	-
		Mean	SD	Mean	SD	Mean	SD	p^† † p***Values between the groups, according to the independent Student t-test. Data are expressed as mean and Standard Deviation (SD); 25(OH)D: 25-hydroxyvitamin D; BMI: Body Mass Index; PTH: Parathyroid Hormone. value
Age (years)	10.61±6.57			11.03±4.400		9.66±10.13		0.6797
BMI (kg/m²)	15.10±2.13			15.38±2.541		14.63±0.93		0.5200
Exposure to sunlight (minutes)	20±19.92			24.54±23.71		11.66±4.02		0.1100

Variables	General (n=18)		Individuals with normal 25(OH)D^* * 25(OH)D ≥75nmoL/L (30ng/mL); levels (n=12)		95% CI	Individuals with low 25(OH)D^ 25(OH)D <75nmoL/L (30ng/mL). levels (n=6)		95% CI	Student t-test
	Mean	SD	Mean	SD	95% CI	Mean	SD	95% CI	p^†value
25(OH)D¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. levels	34.63±13.768		42.15±9.53		36.09–48.02	19.60±6.18		13.11–26.08	<0.001
PTH¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. level	41.1±17.717		42.38±18.25		30.78–53.98	38.53±17.96		19.68–57.38	0.6772
Calcium² 2*** Non-parametric data, comparison between groups according to the Mann–Whitney test presented as medians. level	9,960		10.020		9.87–10.29	9.800		8.59–10.34	0.2161
HMG¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. level	12.91±1.602		13.56±1.26		12.76–14.36	11.53±1.44		10.06–13.09	0.008
HMT¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. level	39.09±4.700		40.85±3.84		38.40–43.29	35.56±4.49		30.84–40.28	0.019
Leukocyte¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. count	10,286.11±3,847.8		8,790.8±2,556.2		7,166.7–10,415	13,277±4,457.1		8,598.4–17,955	0.0114

Genotypes	25(OH)D levels
	Sufficient^* * 25(OH)D ≥75 nmoL/L (30 ng/mL); (n=12)		Insufficient/deficient^ 25(OH)D <75nmoL/L (30ng/mL). (n=6)		Number of participants (18)		OR	95% CI	p
	n	%	n	%	n	%	OR	95% CI	p
FF/FF genotype	8	44	6	33	14	78	0.1453	0.0065–3.212	0.2451
ff genotype	4	22	0		4	22

Variables	FF/Ff genotype (n=14)		95% CI	ff genotype (n=4)		95% CI	Student t-test
Variables	Mean	SD	95% CI	Mean	SD	95% CI	p^†value
25(OH)D¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. level (mg/dL)	32.35±14.02		24.26–40.45	42.60±10.62		25.71–59.49	0.19
PTH¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. level	37.60±13.10		30.03–45.12	53.35±27.80		8.99–97.72	0.11
Calcium² 2*** Non-parametric data, comparison between the groups according to the Mann-Whitney test presented as medians. level	9.925		9.42–10.13	10.16		9.55–10.83	0.27² 2*** Non-parametric data, comparison between the groups according to the Mann-Whitney test presented as medians.
Hemoglobin¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. level	12.59±1.30		11.82–13.33	14.05±2.21		10.53–17.57	0.10
Hematocrit¹ 1*** Parametric data according to normality test, comparison between the groups according to the independent Student t-test. level	38.676±4.11		36.16–40.91	41.00±6.75		30.24–51.75	0.37

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[1] MCR GONÇALVES. E-mail: <mariadaconceicaorgoncalves@gmail.com>.