Stability of vancomycin hydrochloride employed in antimicrobial seal solutions of central intravenous catheters

Barros, Daniele Porto; Onofre, Priscilla Sete de Carvalho; Fonseca, Fernando Luiz Affonso; Rosa, Paulo César Pires; Pedreira, Mavilde da Luz Gonçalves; Peterlini, Maria Angélica Sorgini

doi:10.1590/1518.8345.5869.3621

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Revista Latino-Americana de Enfermagem

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Original Article • Rev. Latino-Am. Enfermagem 30 • 2022 • https://doi.org/10.1590/1518.8345.5869.3621 copy

Stability of vancomycin hydrochloride employed in antimicrobial seal solutions of central intravenous catheters

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Objective:

to verify the stability of vancomycin hydrochloride in antimicrobial seal solutions with and without association of heparin sodium according to temperature and association time.

Method:

an experimental study designed for the analysis of hydrogenionic potential and concentration by means of high-efficiency liquid chromatography of vancomycin hydrochloride (n=06) and vancomycin hydrochloride and heparin sodium (n=06). The solutions studied were submitted to absence of light, as well as to 22°C and 37°C. Analyses in triplicate (n=192) were performed at the initial moment (T0) and three (T3), eight (T8) and 24 hours (T24) after preparation. The data were submitted to analysis of variance (p≤0.05).

Results:

concentration of the antimicrobial at 22°C presented a reduction (T0-T8) and a subsequent increase (T24); hydrogenionic potential decreased significantly over time. At 37°C, the concentration increased up to T3 and decreased at T24, with a reduction of hydrogenionic potential up to 24 hours. Concentration of the vancomycin hydrochloride and heparin sodium solutions varied with a reduction at 22°C, accompanied by increased hydrogenionic potential. Precipitate formation was observed by visual inspection of the vancomycin hydrochloride-heparin sodium association (T3).

Conclusion:

pharmacological stability of vancomycin hydrochloride (5 mg/mL) and physical incompatibility with heparin sodium (100 IU/mL) were evidenced after three hours of association in the antimicrobial seal solutions studied.

Descriptor:
Catheter-Related Infections; Drug Stability; Infusions, Intravenous; Vancomycin; Heparin; Nursing

Temperature	Type of solution	Moment	Concentration (%) Mean ± SD (Min-Max)	Concentration (mg/mL) Mean ± SD (Min-Max)	Hydrogenioninc potential Mean ± SD (Min-Max)
22ºC	Vancomycin hydrochloride solution	T0	101.93 ± 1.66*	5.10 ± 0.08*	3.76 ± 0.05*
		T0	(100.75-104.33)	(5.04-5.22)	(3.73-3.82)
		T3	101.45 ± 1.46	5.07 ± 0.07	3.73 ± 0.01*
		T3	(99.54-103.58)	(4.98-5.18)	(3.73-3.74)
		T8	100.31 ± 0.87^†	5.02 ± 0.04^†	3.74 ± 0.01*
		T8	(99.39-101.39)	(4.97-5.07)	(3.73-3.75)
		T24	102.50 ± 1.46*	5.12 ± 0.07*	3.59 ± 0.03^†
		T24	(100.52-104.44)	(5.03-5.22)	(3.56-3.62)
		p	<0.05	<0.05	<0.05
	Vancomycin hydrochloride and heparin sodium solution	T0	101.73 ± 3.81*	5.09 ± 0.19*	3.63 ± 0.03^†
		T0	(95.50-106.64)	(4.78-5.33)	(3.60-3.65)
		T3	99.01 ± 1.56^†	4.95 ± 0.08^†	3.59 ± 0.02^†
		T3	(97.49-101.24)	(4.87-5.06)	(3.58-3.61)
		T8	97.38 ± 1.20^†	4.87 ± 0.06^†	3.64 ± 0.04^†
		T8	(95.60-98.80)	(4.78-4.94)	(3.61-3.69)
		T24	96.34 ± 1.70^†	4.82 ± 0.08^†	4.00 ± 0.02*
		T24	(94.36-99.44)	(4.72-4.97)	(3.99-4.02)
		p	<0.05	0.05	<0.05
37ºC	Vancomycin hydrochloride solution	T0	97.79 ± 0.69^†	4.89 ± 0.03^†	3.73 ± 0.02*
		T0	(96.99-98.97)	(4.85-4.95)	(3.71-3.74)
		T3	101.96 ± 1.13*	5.10 ± 0.06*	3.69 ± 0.02^†
		T3	(100.25-102.92)	(5.01-5.15)	(3.68-3.71)
		T8	100.73 ± 1.69*	5.04 ± 0.08*	3.67 ± 0.01^†
		T8	(98.77-102.58)	(4.94-5.13)	(3.66-3.68)
		T24	98.35 ± 2.80^†	4.92 ± 0.14^†	3.68 ± 0.02^†
		T24	(95.41-101.83)	(4.77-5.09)	(3.66-3.69)
		p	<0.05	<0.05	0.05
	Vancomycin hydrochloride and heparin sodium solution	T0	94.91 ± 1.76^†	4.75 ± 0.09^†	3.80 ± 0.02
		T0	(92.68-96.80)	(4.63-4.84)	(3.78-3.82)
		T3	95.12 ± 1.29^†	4.76 ± 0.06^†	3.75 ± 0.07
		T3	(93.19-96.80)	(4.66-4.84)	(3.68-3.82)
		T8	95.05 ± 3.15^†	4.75 ± 0.16^† (4.54-4.97)	3.75 ± 0.04
		T8	(90.84-99.43)	4.75 ± 0.16^† (4.54-4.97)	(3.72-3.79)
		T24	96.94 ± 1.23*	4.85 ± 0.06*	3.78 ± 0.01
		T24	(94.94-98.42)	(4.75-4.92)	(3.78-3.79)
		p	0.001	0.001	0.389

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[1] Corresponding author
Daniele Porto Barros. E-mail: danipbarros@yahoo.com.br