The aim of this study was to analyze the effect of transdiaphragmatic blockage due to acute infectious peritonitis, induced by inoculation of predetermined qualitative and quantitative bacterial suspension analysis. Forty-one adult, male, Wistar rats were studied. Their weight vary from 118 to 399 g. The animals were allocated into two groups: group A or control group (n=19), and group B or experimental group (n=22). The animals in group B. after inalatory anesthetic, were submitted to laparotomy and diaphragmatic blockage of the peritoneum surface with celluloid membrane and kept under ad libitum conditions for fifteen days. After this period of time, both groups received a percutaneous inoculation of bacterial suspension in the peritoneal cavity containing Pseudomonas aeruginosa 2,7 x l0(9) UFC/ml (American Type Culture Collection - ATCC 25853). The dosage was 1 ml of the suspension for every 100 g of weight. In every death, the animal was submitted to necropsy for peritoneal and pleural cavity macroscopic analysis, as well as pleural effusion and intracardiac blood collected for culture and bacterial analysis. The survivors animals were sacrificed after forty-eight hours and also submitted to necropsy and material collection for bacteriologic evaluation. It was verified that all the animals had objective clinical signs of sepsis in evolution. The incidence of pleural effusion observed in the control group and experimental group was, respectively, 18 (94,7%) and 8 (36,4%), (p=0.0001). To study the survival rate, the Kaplan-Meier method was used, and the result was a higher survival rate in group B, (p=0,024; p=0.0211). The present study demonstrated that the animals submitted to previous transdiaphragmatic blockage with celluloid membrane, had a higher survival rate and less frequency of pleural effusion, statistically significant. when compared with the animals not submitted to the blockage.
Peritonitis; Peritoneal cavity; Diaphragmatic lymphatics