ABSTRACT
Macrophomina pseudophaseolina has recently been reported in association with weeds in melon producing areas in Northeastern Brazil. Species from this genus are the causal agents of root rot and vine decline (RRVD) in melon, reducing its productivity. It is needed to know the genetic variability of the pathogen to develop effective control methods. Thus, this work aimed to assess the genetic diversity among M. pseudophaseolina isolates collected from the weeds Trianthema portulacastrum L. and Boerhavia diffusa L. using ISSR and RAPD markers. For this, 41 M. pseudophaseolina isolates were submitted to amplification with five ISSR and ten RAPD primers. Genetic similarity was analyzed using the Jaccard’s coefficient and cluster analysis was performed by the UPGMA method. Combining data from both markers, the 41 isolates were separated into eight groups. Most groups were not arranged according to geographical origin and host of the pathogen. The genetic similarity among isolates ranged from 0.15 to 0.87. On the other hand, the highest genetic dissimilarity (85%) was observed between the isolate MpBr11, collected from T. portulacastrum in Icapuí (CE), and MpBr65, collected from B. diffusa in Assú (RN). Results obtained herein can assist breeding programs for the selection of resistance sources and the development of effective control methods against RRVD in melon.
Keywords:
Genetic variability; RAPD; ISSR; Melon
