Development and characterization of myofibrillar protein film obtained from Nile tilapia mechanically separated meat (MSM)<sup/>

Leite, Lana Oliveira; Nogueira, Sara Monaliza Sousa; Silva, Ana Irene Martins da; Vale, Diego Alves do; Cerqueira, Davi Araújo; Silva, André Luis Coelho da; Souza Filho, Men de Sá Moreira de; Souza, Bartolomeu Warlene Silva de

doi:10.5935/1806-6690.20240036

Lana Oliveira Leite

Postgraduate Program in Fisheries Engineering, Federal University of Ceará (UFC), Campus do Pici s/n, Bloco: 827 60455-970, Fortaleza-CE, Brazil

http://orcid.org/0000-0002-0059-2785

Sara Monaliza Sousa Nogueira

Postgraduate Program in Fisheries Engineering, Federal University of Ceará (UFC), Campus do Pici s/n, Bloco: 827 60455-970, Fortaleza-CE, Brazil

http://orcid.org/0000-0003-4310-8655

Ana Irene Martins da Silva

Postgraduate Program in Fisheries Engineering, Federal University of Ceará (UFC), Campus do Pici s/n, Bloco: 827 60455-970, Fortaleza-CE, Brazil

http://orcid.org/0000-0001-6697-6901

Diego Alves do Vale

Postgraduate Program in Fisheries Engineering, Federal University of Ceará (UFC), Campus do Pici s/n, Bloco: 827 60455-970, Fortaleza-CE, Brazil

http://orcid.org/0000-0002-6388-155X

Davi Araújo Cerqueira

Postgraduate Program in Fisheries Engineering, Federal University of Ceará (UFC), Campus do Pici s/n, Bloco: 827 60455-970, Fortaleza-CE, Brazil

http://orcid.org/0009-0002-5921-0945

André Luis Coelho da Silva

Postgraduate Program in Natural Resources Biotechnology, Federal University of Ceará (UFC), Campus do Pici s/n, Bloco 873, 60455-970, Fortaleza-CE, Brazil

http://orcid.org/0000-0003-0685-6398

Men de Sá Moreira de Souza Filho

Embrapa Agroindustry Tropical, Brazilian Agricultural Research Corporation (Embrapa) - Rua Dra. Sara Mesquita, 2270, Pici, 60511-110, Fortaleza-CE, Brazil

http://orcid.org/0000-0003-4404-2360

Bartolomeu Warlene Silva de Souza ^**Author for correspondence

Fisheries Engineering Department, Federal University of Ceará (UFC), Campus do Pici s/n, Bloco 827, 60455-970, Fortaleza-CE, Brazil

http://orcid.org/0000-0003-4007-2079

*Author for correspondence

1
This study was supported by the Brazilian National Council for Scientifi c and Technological Development (CNPq), Coordination of Improvement of Higher Education Personnel (CAPES), Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP) and Brazilian Agricultural Research Corporation (EMBRAPA)

Treatments	0.0% glycerol	0.1% glycerol	0.2% glycerol
1.0% MP	^T1	T₄	T₇
1.5% MP	t₂	T₅	T₈
2.0% MP	T₃	T₆	T₉

	MSM (%)	MP (%)
Moisture	75.90 ± 0.12	-
Proteins	12.92 ± 0.16	87.33 ± 0.77
Ashes	0.96 ± 0.021	9.46 ± 0.26
Lipids	9.80 ± 0.29	1.68 ± 0.57
Carbohydrates	0.64 ± 0.05	-

Amino acids	mg g^-1 protein	FAO/WHO standard (mg g^-1 protein)
alanine	53.20 + 0.12	-
glutamic acid	121.80 + 0.04	-
aspartic acid	120.50 + 0.05	-
serine	44.30 + 0.06	-
glycine	43.80 + 0.10	-
histidine^ Essential amino acids.	26.30 + 0.07	15.00
arginine^ Essential amino acids.	27.40 + 0.05	-
threonine^ Essential amino acids.	46.90 + 0.08	23.00
tyrosine	34.20 + 0.03	-
tryptophan^* * Not determined.	-	6.00
valine^ Essential amino acids.	63.70 + 0.12	39.00
methionine^ Essential amino acids.	31.70 + 0.03	16.00
cysteine	7.00 + 0.02	6.00
isoleucine^ Essential amino acids.	59.30 + 0.06	30.00
leucine^ Essential amino acids.	84.10 + 0.01	59.00
phenylalanine^ Essential amino acids.	42.00 + 0.06	30.00^* ***** phenylalanine + tyrosine Source: The authors
lysine^ Essential amino acids.	104.20 + 0.04	45.00
proline	89.20 + 0.09	-

Concentration	WVP (g/m.day.atm)	Solubility (%)	Thickness (mm)
1% MP 0.0% G	0.61 ± 0.49 a	17.09 ± 0.32 a	0.084 a
1% MP 0.1% G	0.66 ± 0.27 a	17.93 ± 0.24 a	0.086 a
1% MP 0.2% G	0.71± 0.73 a	17.98 ± 0.13 a	0.109 a
1.5% MP 0.0% G	0.86 ± 0.96 ab	18.09 ± 0.32 ab	0.166 bc
1.5% MP 0.1% G	0.87 ± 0.82 ab	18.13 ± 0.39 b	0.162 b
1.5% MP 0.2% G	0.90 ± 0.22 bcd	19.27 ± 0.23 b	0.167 bc
2% MP 0.0% G	0.93 ± 0.12 bcd	20.09 ± 0.25 c	0.178 bc
2% MP 0.1% G	0.94 ± 0.24 bcd	21.03 ± 0.14 c	0.168 bc
2% MP 0.2% G	1.04 ± 0.53 de	22.27 ± 0 .09 c	0.186 bc

Concentration	Strain at break (MPa)	Tensile strength (MPa)	Elastic modulus (MPa)
1% MP 0.0% G	77.45 ± 0.44 a	10.86 ± 0.99 a	86.18 ± 0.13 a
1% MP 0.1% G	177.14 ± 0.23 b	4.64 ± 0.89 b	29.13 ± 0.09 b
1% MP 0.2% G	155.11 ± 0.78 b	4.40 ± 0.23 b	28.19 ± 0.44 b
1.5% MP 0.0% G	1.68 ± 0.13 c	12.01 ± 0.76 ace	874.39 ± 0.58 c
1.5% MP 0.1% G	47.37 ± 0.69 d	7.44 ± 0.77 d	377.7± 0.28 d
1.5% MP 0.2% G	163.69 ± 0.59 be	4.94 ± 0.23 bd	33.83 ± 0.53 e
2% MP 0.0% G	0.81 ± 0.78 fc	6.91 ± 0.55 d	600.09 ± 0.24 f
2% MP 0.1% G	6.12 ± 0.77 fc	10.20 ± 1.66 ae	434.84 ± 0.23 g
2% MP 0.2% G	1.51 ± 0.29 fc	13.61 ± 0 .93 acef	105.51 ±1.03 h

Concentration	L^*	a^*	b^*	opacity (%)
1% MP 0.0% G	85.64 ± 0.12 a	1.57 ± 0.45 a	0.31 ± 0.34 a	273.07 ± 0.64 a
1% MP 0.1% G	85.44 ± 0.19 a	1.24 ± 0.23 b	0.30 ± 0.44 a	273.18 ± 0.38 a
1% MP 0.2% G	86.09 ± 0.67 a	1.57 ± 0.32 ac	0.31 ± 0.34 a	275.40 ± 0.54 a
1.5% MP 0.0% G	88.45 ± 0.55 b	1.74 ± 0.34 acd	0.35 ± 0.67 a	284.65 ± 0.22 b
1.5% MP 0.1% G	88.45 ± 0.44 b	1.74 ± 0.45 de	0.35 ± 0.12 a	284.65 ± 0.56 b
1.5% MP 0.2% G	88.42 ± 0.33 b	1.76 ± 0.34 def	0.29 ± 0.18 a	283.23 ± 0.22 b
2% MP 0.0%G	90.67 ± 0.89 c	1.90 ± 0.67 defg	-1.33 ± 0.98 b	300.46 ± 0.94 c
2% MP 0.1% G	95.45 ± 1.34 d	1.89 ± 0.56 defg	-1.33 ± 1.04 b	296.01 ± 1.04 c
2% MP 0.2% G	90.67 ± 1.34 ce	1.90 ± 0.45 defg	-1.33 ± 1.04 b	296.01 ± 0.97 c

[1] *Author for correspondence

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