A study of phylogenetic relationships of the genera of Mesembrinellinae and of the species of Eumesembrinella Townsend, 1931, on the ground of shared derived character states (synapomorphies) is presented. Resulting the following assumption: Laneella Mello, 1967 presenting sister group relationship with all other Mesembrinellinae, followed by Souzalopesiella Guimarães, 1977, M. peregrina Aldrich, 1922 that was isolated from Mesembrinella Giglio-Tos, 1893. Mesembrinella was considered paraphiletic, its species were judged in changeable positions at the bifurcation point of analysis of Albuquerquea Mello, 1967 with the tricotomic interrelationships of Thompsoniella Guimarães, 1977, Huascaromusca Townsend, 1918 and Eumesembrinella Townsend, 1931. The phylogeny of the genus Eumesembrinella shows E. cyaneicincta in basal position, followed by E. quadrilineata that showed sister group relationship wilh E. randa plus E. benoisti. Mesembrinella currani Guimarães, 1977 and Eumesembrinella benoisti (Séguy 1925) were revalided.
Calliphoridae; Mesembrinellinae; Eumesembrinella; classification; phylogeny