Ultrastructure of ovarian follicular epithelium of the amazonian fish Pseudotylosurus microps (Gunther) (Teleostei, Belonidae): I. the follicular cells cycle of development

Cruz-Landim, Carminda da; Cruz-Hofling, Maria Alice da

doi:10.1590/S0101-81752001000100010

Abstract

The present paper deals with the changes on ultrastructural features of the follicular cells along the growth of the oocyte of Pseudotylosurus microps (Gunther, 1868). The epithelium pass from single squamous to pseudostratified and finally cylindric. Remarkable are the changes in the nuclear shapes, the increasing amount of rough endoplasmic reticulum and the formation of large lipid deposits in the follicular cells by the end of vitellogenic phase. Very peculiar intercellular deposits forms between the perinucleolar and vitellogenic phase of oocyte maturation. Although largely referred to in the specialized literature, our attempts to correlate the follicular cells changes with the formation of the oocyte envelopes or even with an endocrine function are not supported by conclusive evidences in the present paper.

Pseudotylosurus microps; follicular epithelium; ultrastructure; teleost

Ultrastructure of ovarian follicular epithelium of the amazonian fish Pseudotylosurus microps (Gunther) (Teleostei, Belonidae). I. The follicular cells cycle of development

Carminda da Cruz-Landim^I; Maria Alice da Cruz-Hofling^II

^IDepartamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista. 13506-900 Rio Claro, São Paulo, Brasil

^IIDepartamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas. 13083-970 Campinas, São Paulo, Brasil

ABSTRACT

The present paper deals with the changes on ultrastructural features of the follicular cells along the growth of the oocyte of Pseudotylosurus microps (Gunther, 1868). The epithelium pass from single squamous to pseudostratified and finally cylindric. Remarkable are the changes in the nuclear shapes, the increasing amount of rough endoplasmic reticulum and the formation of large lipid deposits in the follicular cells by the end of vitellogenic phase. Very peculiar intercellular deposits forms between the perinucleolar and vitellogenic phase of oocyte maturation. Although largely referred to in the specialized literature, our attempts to correlate the follicular cells changes with the formation of the oocyte envelopes or even with an endocrine function are not supported by conclusive evidences in the present paper.

Key words:Pseudotylosurus microps, follicular epithelium, ultrastructure, teleost

Full text available only in PDF format.

ACKNOWLEDGEMENTS. Identification of the specimens by Dr. Naércio Menezes and Dr. Heraldo Britski is gratefully acknowledged. This work was supported by grants from FAPESP, FINEP, CNPq and FAEP-UNICAMP.

Recebido em 28.X.1999; aceito em 29.III.2001

Anderson, H. 1966. A study of the fibrillar appendages associated with the surface of eggs of the killifish, Fundulus heteroclitus. Anat. Rec. 154:308-309.
______. 1967. The formation of the primary envelope during oocyte differentiation in teleosts. Jour. Cell Biol. 35:193-212.
______. 1974. Comparative aspects of ultrastructure of female gamete. Int. Rev. Cytol. 4(Suppl):1-70.
Anderson, W.A. & A. Spielman. 1971. Permability of the ovarian follicle of Aedes aegypti mosquitos. Jour. Cell Biol. 50:201-221.
Britsch, J. 1980. Ultrastructure de l'épithelium folliculaire et des enveloppes de l'ovocyte chez Lepismachilis targionii (Grassi) (Thysanura: Machilidae). Int. Jour. Insect Morphol. Embryol. 9:25-40.
Busson-Mabillot, S. 1977. Un type particulier de sécrétion exocrine: Celui de l'appareil adhésif de l'oeuf d'un poisson téléostéen. Biol. Cellulaire 30:233-244.
Chaudry, H.S. 1956. The origin and structure of the zona pellucida in the ovarian eggs of teleosts. Z. Zellforsch. 43:478-485.
Cruz-Landim, C. 1990. Aspectos ultra-estruturais das células produtoras de esteróides do ovário de Colossoma mitrei (Teleostei: Characidae). Colóquio da Soc. Brasil. Mie. Eletr.: 255-256.
Cruz-Landim, C. & M.A, Cruz-Höfling. 1989a. Electron microscopic studies on the development of the chorion of Astyanax bimaculatus (Teleostei: Characidae). Zool. Jb. Anat. 119:241-249.
______. 1989b. Estudo ao microscópio eletrônico da deposição do envoltório do ovócito de peixes. I. Plagiosoium squamosissimus (Teleostei-Scianidae). Naturalia 14:97-105.
______. 1999a. Fine structure of previtellogenic oocytes in Pseudolylosurus microps (Teleostei: Belonidae). Braz. Jour. Morphol. Sci. 16:167-173.
______. 1999b. Ultrastructure of the oocyte vitelline envelope deposition in the Amazonian fish Pseudolylosurus microps (Teleostei: Belonidae). Acta Microscopica 8:23-28.
Cruz-Höfling, M.A. & C. Cruz-Landim. 1993. Differentiation of the follicular epithelium during oocyte growth in the Amazonian fish, Crenicichla Johanna (Teleostei: Cichlidae). Zool. Jb. Anat. 123:59-74.
Dumont, D. & R. Brummet. 1980. The vitelline envelope, chorion, and micropyle of Fundulus heteroclitus eggs. Gamete Res. 3:25-44.
Favard-Sereno, C. 1971. Cycles sécrétoires successifs au cours de l'élaboration des enveloppes de l'ovocyte chez le Grillon (Insect, Orthoptere). Rôle de l'appareil de Golgi. Jour. Microscopic 11:401-424.
Flügel, H. 1967. Lich-und elektronenmikroscopische untersuchungen an oozyten und eiern einiget knochenfische. Z. Zellforsch. 83:82-116.
Hart, N.H.; R. Pietri & M. Donovan. 1984. The structure of the chorion and associated surface filaments in Oryzias evidence for the presence of extracellular tubules. Jour. Exp. Zool. 230:273-296.
Hyllner, S.J. & C. Haux. 1992. Immunochemical of major vitelline envelope proteins in the plasma and oocytes of the maturing female rainbow trout, Oncorhynchus mykies. Jour. Endocrinol. 135:303-309.
Hirosr, H. 1972. The ultrastructure of the ovarian follicle of medaka Oryzias latipes. Z. Zellforsch. 123:316-329.
Iwasaki, Y. 1973. Histochemical detection of - 3 - hydroxysteroid dehydrogenase in the ovary of medaka, Oryzias latipes, during annual reproductive cycle. Bull. Fac. Fish. Hokkaido Univ. 23:177-184.
Khoo, K.H. 1975. The corpus luteum of gold fish Carassius auratus L. and its functions. Can. Jour. Zool. 53:1306-1323.
Lambert, J.G. 1970. The ovary of the guppy Poecilia reticulata. The granulosa cells as sites of steroid biosynthesis. Gen. Comp. Endocrinol. 15:464-476.
Lopes, R.A.; H.S.L. Santos; J.R.V Costa; M.G. Pelizaro & N. Castagnoli. 1982. Histochemical study of oocyte zona radiata of the lambari Astyanax bimaculatus lacustris - Linnaeus, 1758 (Osteichthyes: Characidae). Zool. Anz. 208:265-268.
Nichols, T.J. & G. Maple. 1972. Ultrastructural observations on possible sites of steroid biosynthesis in the ovarian follicular epithelium of two species of cichlid fish Cichlasoma nigrofascialitum and Haplochomie multicolor. Z. Zellforsch. 128:317-335.
Schackley, S.E. & P.E. King. 1977. Oogenesis in a marine teleost, Blenniuspholis L. Cell Tissue Res. 181:105-128.
Shelton, W.L. 1978. Fate of the follicular epithelium in Dorosoma petenense (Pisces: Clupeidae). Copeia 2:237-244.
Tang, F.; S.T.H. Chan & B. Lofts. 1975. A study on the 3B - and 17B hydroxysteroid dehydrogenase activities in the gonads of Monopterus albus (Pisces: Teleostei) at various sexual phases during natural sex reversal. Jour. Zool. 175:571-580.
Wourms, J.P. & H. Sheldon. 1976. Annual fish oogenesis. II. Formation of the secondary egg envelope. Dev. Biol. 50:355-366.

Publication Dates

Publication in this collection
30 Apr 2009
Date of issue
Mar 2001

History

Accepted
29 Mar 2001
Received
28 Oct 1999

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] Anderson, H. 1966. A study of the fibrillar appendages associated with the surface of eggs of the killifish, Fundulus heteroclitus. Anat. Rec. 154:308-309.

[2] ______. 1967. The formation of the primary envelope during oocyte differentiation in teleosts. Jour. Cell Biol. 35:193-212.

[3] ______. 1974. Comparative aspects of ultrastructure of female gamete. Int. Rev. Cytol. 4(Suppl):1-70.

[4] Anderson, W.A. & A. Spielman. 1971. Permability of the ovarian follicle of Aedes aegypti mosquitos. Jour. Cell Biol. 50:201-221.

[5] Britsch, J. 1980. Ultrastructure de l'épithelium folliculaire et des enveloppes de l'ovocyte chez Lepismachilis targionii (Grassi) (Thysanura: Machilidae). Int. Jour. Insect Morphol. Embryol. 9:25-40.

[6] Busson-Mabillot, S. 1977. Un type particulier de sécrétion exocrine: Celui de l'appareil adhésif de l'oeuf d'un poisson téléostéen. Biol. Cellulaire 30:233-244.

[7] Chaudry, H.S. 1956. The origin and structure of the zona pellucida in the ovarian eggs of teleosts. Z. Zellforsch. 43:478-485.

[8] Cruz-Landim, C. 1990. Aspectos ultra-estruturais das células produtoras de esteróides do ovário de Colossoma mitrei (Teleostei: Characidae). Colóquio da Soc. Brasil. Mie. Eletr.: 255-256.

[9] Cruz-Landim, C. & M.A, Cruz-Höfling. 1989a. Electron microscopic studies on the development of the chorion of Astyanax bimaculatus (Teleostei: Characidae). Zool. Jb. Anat. 119:241-249.

[10] ______. 1989b. Estudo ao microscópio eletrônico da deposição do envoltório do ovócito de peixes. I. Plagiosoium squamosissimus (Teleostei-Scianidae). Naturalia 14:97-105.

[11] ______. 1999a. Fine structure of previtellogenic oocytes in Pseudolylosurus microps (Teleostei: Belonidae). Braz. Jour. Morphol. Sci. 16:167-173.

[12] ______. 1999b. Ultrastructure of the oocyte vitelline envelope deposition in the Amazonian fish Pseudolylosurus microps (Teleostei: Belonidae). Acta Microscopica 8:23-28.

[13] Cruz-Höfling, M.A. & C. Cruz-Landim. 1993. Differentiation of the follicular epithelium during oocyte growth in the Amazonian fish, Crenicichla Johanna (Teleostei: Cichlidae). Zool. Jb. Anat. 123:59-74.

[14] Dumont, D. & R. Brummet. 1980. The vitelline envelope, chorion, and micropyle of Fundulus heteroclitus eggs. Gamete Res. 3:25-44.

[15] Favard-Sereno, C. 1971. Cycles sécrétoires successifs au cours de l'élaboration des enveloppes de l'ovocyte chez le Grillon (Insect, Orthoptere). Rôle de l'appareil de Golgi. Jour. Microscopic 11:401-424.

[16] Flügel, H. 1967. Lich-und elektronenmikroscopische untersuchungen an oozyten und eiern einiget knochenfische. Z. Zellforsch. 83:82-116.

[17] Hart, N.H.; R. Pietri & M. Donovan. 1984. The structure of the chorion and associated surface filaments in Oryzias evidence for the presence of extracellular tubules. Jour. Exp. Zool. 230:273-296.

[18] Hyllner, S.J. & C. Haux. 1992. Immunochemical of major vitelline envelope proteins in the plasma and oocytes of the maturing female rainbow trout, Oncorhynchus mykies. Jour. Endocrinol. 135:303-309.

[19] Hirosr, H. 1972. The ultrastructure of the ovarian follicle of medaka Oryzias latipes. Z. Zellforsch. 123:316-329.

[20] Iwasaki, Y. 1973. Histochemical detection of - 3 - hydroxysteroid dehydrogenase in the ovary of medaka, Oryzias latipes, during annual reproductive cycle. Bull. Fac. Fish. Hokkaido Univ. 23:177-184.

[21] Khoo, K.H. 1975. The corpus luteum of gold fish Carassius auratus L. and its functions. Can. Jour. Zool. 53:1306-1323.

[22] Lambert, J.G. 1970. The ovary of the guppy Poecilia reticulata. The granulosa cells as sites of steroid biosynthesis. Gen. Comp. Endocrinol. 15:464-476.

[23] Lopes, R.A.; H.S.L. Santos; J.R.V Costa; M.G. Pelizaro & N. Castagnoli. 1982. Histochemical study of oocyte zona radiata of the lambari Astyanax bimaculatus lacustris - Linnaeus, 1758 (Osteichthyes: Characidae). Zool. Anz. 208:265-268.

[24] Nichols, T.J. & G. Maple. 1972. Ultrastructural observations on possible sites of steroid biosynthesis in the ovarian follicular epithelium of two species of cichlid fish Cichlasoma nigrofascialitum and Haplochomie multicolor. Z. Zellforsch. 128:317-335.

[25] Schackley, S.E. & P.E. King. 1977. Oogenesis in a marine teleost, Blenniuspholis L. Cell Tissue Res. 181:105-128.

[26] Shelton, W.L. 1978. Fate of the follicular epithelium in Dorosoma petenense (Pisces: Clupeidae). Copeia 2:237-244.

[27] Tang, F.; S.T.H. Chan & B. Lofts. 1975. A study on the 3B - and 17B hydroxysteroid dehydrogenase activities in the gonads of Monopterus albus (Pisces: Teleostei) at various sexual phases during natural sex reversal. Jour. Zool. 175:571-580.

[28] Wourms, J.P. & H. Sheldon. 1976. Annual fish oogenesis. II. Formation of the secondary egg envelope. Dev. Biol. 50:355-366.