The first record of Histiotus laephotis Thomas, 1916 from Brazil is reported here. An adult male was caught with a mist net in a barn located within an Araucaria pine forest in the municipality of Passos Maia, State of Santa Catarina, Southern Brazil (26º46'48"S and 52º03'34"W). This record extends the distribution of this species in South America. Morphological and taxonomic notes of this species are described as well.
Atlantic Forest; geographic distribution; Histiotus macrotus laephotis
