Atividade antimicrobiana de <i>Stomoxys calcitrans</i>contra isolados de <i>Beauveria bassiana sensu lato</i>

Moraes, Ana Paula Rodrigues de; Salles, Cristiane Martins Cardoso de; Bittencourt, Vânia Rita Elias Pinheiro; Bittencourt, Avelino José

doi:10.1590/S1984-29612015064

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Original Article • Rev. Bras. Parasitol. Vet. 24 (3) • Jul-Sep 2015 • https://doi.org/10.1590/S1984-29612015064 copiar

Atividade antimicrobiana de Stomoxys calcitranscontra isolados de Beauveria bassiana sensu lato

Autoria SCIMAGO INSTITUTIONS RANKINGS

Este estudo teve como objetivos avaliar as características antimicrobianas de larvas de Stomoxys calcitrans (Diptera: Muscidae) contra os isolados CG138, CG228 e ESALQ986 de Beauveria bassiana sensu lato (Balsamo-Crivelli) Vuillemin, 1912 (Hypocreales: Cordycipitaceae). Ovos, larvas e pupas de S. calcitrans foram expostos a estes mesmos isolados. Após análise estatística, foi verificado que os estágios imaturos de S. calcitrans não foram susceptíveis aos isolados utilizados, independentemente do método de exposição utilizado. O teste de difusão em meio sólido mostrou que quando o isolado CG138 foi exposto a macerado de larvas houve redução do desenvolvimento fúngico. A análise dos perfis cromatográficos mostrou que o macerado ou muco de larvas do grupo controle e dos grupos expostos ao isolado CG138 apresentaram diferenças nos perfis. Um desenvolvimento reduzido do isolado CG138 na cutícula de larvas foi observado pela microscopia eletrônica de varredura.

Stomoxys calcitrans ; Beauveria bassiana ; atividade antimicrobiana; controle biológico

Isolates/Concentrations		First Assay
		Eggs ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.	Larvae ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.	Pupae ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.	Eggs ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.	Larvae ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.	Pupae ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.
		CM (%)	CM (%)	CM (%)	CM (%)	CM (%)	CM (%)
Control		0	0	0	0	0	0
CG 138	2x5⁵ con.ml^-1	-28.2	0	11.1	-18,5	12.0	4.0
	2x5⁶ con.ml^-1	-5.3	4.5	25.9	0	31.0	-3.9
	2x5⁷ con.ml^-1	-14.2	18.1	-7,4	3.7	32.0	0
	2x5⁸con.ml^-1	-3.6	4.5	11.1	7.4	28.0	4.0
CG 228	2x5⁵ con.ml^-1	10.7	-4.5	3.7	7.4	0	20.0
	2x5⁶ con.ml^-1	-8.9	-27.2	0	9.2	-12.0	12.0
	2x5⁷ con.ml^-1	7.1	-13.6	3.7	-33.3	8.0	-8.0
	2x5⁸con.ml^-1	8.9	4.5	11.1	0	20.0	-11.9
ESALQ 986	2x5⁵ con.ml^-1	-14.9	-3.6	-	17.6	6.7	-
	2x5⁶ con.ml^-1	8.9	-3.6	-	7.3	3.3	-
	2x5⁷ con.ml^-1	6.0	7.1	-	-4.4	13.3	-
	2x5⁸con.ml^-1	1.5	7.1	-	16.2	13.3	-

Isolates/Concentrations		First Assay
		Eggs ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.	Larvae ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.	Eggs ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.	Larvae ^* * = No statistical difference between the mortality percentages in the treated groups and control group in each immature stage of Stomoxys calcitrans.
		CM (%)	CM (%)	CM (%)	CM (%)
Control		0	0	0	0
CG 138	2x5⁸con.ml^-1	-16.3	0	-21.8	-12.5
CG 228	2x5⁸con.ml^-1	-5.4	-11.5	-5.4	-16.7
ESALQ 986	2x5⁸con.ml^-1	-5.4	-7.7	0	-16.7

Larval Exposure Method	Isolate	Concentrations	Emergence
Larval Exposure Method	Isolate	Concentrations	First Assay	Second Assay
First^* * = No statistical difference between the emergence percentages in the treated groups and control group of Stomoxys calcitrans.	CG 138	Control	63.3	60.0
		2x5⁵ con.ml^-1	46.6	53.3
		2x5⁶ con.ml^-1	56.6	36.6
		2x5⁷ con.ml^-1	46.6	43.3
		2x5⁸con.ml^-1	63.3	43.3
	CG 228	Control	63.3	60.0
		2x5⁵ con.ml^-1	66.6	66.6
		2x5⁶ con.ml^-1	63.3	73.3
		2x5⁷ con.ml^-1	63.3	60.0
		2x5⁸con.ml^-1	60.0	53.3
	ESALQ 986	Control	80.0	90.0
		2x5⁵con.ml^-1	90.0	86.6
		2x5⁶con.ml^-1	90.0	73.3
		2x5⁷con.ml^-1	83.3	73.3
		2x5⁸con.ml^-1	86.6	86.6
Second^* * = No statistical difference between the emergence percentages in the treated groups and control group of Stomoxys calcitrans.	C	Control	76.6	80.0
	CG 138	2x5⁸con.ml^-1	83.3	76.6
	CG 228	2x5⁸con.ml^-1	86.6	86.6
	ESALQ 986	2x5⁸con.ml^-1	86.6	86.6

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[1] *Corresponding author: Avelino José Bittencourt. Departamento de Medicina, Instituto de Veterinária e Cirurgia Veterinária, Universidade Federal Rural do Rio de Janeiro – UFRRJ, BR 465 Km 7, CEP 23890-000, Seropédica, RJ, Brasil. e-mail: bittenc@ufrrj.br