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Revista Brasileira de Parasitologia Veterinária

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Original Article • Rev. Bras. Parasitol. Vet. 31 (2) • 2022 • https://doi.org/10.1590/S1984-29612022020 copy

Immunophenotypical and pathological changes in dogs experimentally infected with Ehrlichia canis

Alterações imunofenotípicas e patológicas em cães experimentalmente infectados por Ehrlichia canis

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstract

Canine monocytic ehrlichiosis (CME) is one of the most important tick-borne diseases worldwide, with multisystemic presentations. Immune dysregulation has been proposed as the primary mechanism involved in its pathogenesis and in tissue injury in dogs with CME. Experimental infection of German Shepherd dogs in the present study demonstrated that CME caused marked pathological changes in their lymph nodes and spleen, and also gave rise to mononuclear infiltration in organs and tissues. Immunophenotyping of cells in lymph nodes, spleen and injured tissues highlighted differences in lymphocyte subsets, local expression of immunoglobulin subclasses and MHCII molecules between infected and control dogs. These findings suggest that the immunophenotypic and immunopathological changes in dogs with acute experimental CME are related to Th1 bias and compartmentalized immune response.

Keywords:
Monocytic ehrlichiosis; canine; tick-borne disease; infection; immunity

Antigen	Antibody clone^*,◊,§,★	Dilution	Source
CD79α	HM57	1:20	Dako Corp.
IgG1	Canine IgG1, polyclonal	1:3000	Bethyl Labs.
IgG2	Canine IgG2, polyclonal	1:2000	Bethyl Labs.
IgE	Canine IgE, polyclonal	1:600	Bethyl Labs.
CD3	Polyclonal	1:200	Dako Corp.
Myeloid/histiocyte	MAC387	1:200	Dako Corp.
CD68	PG-M1	1:50	Dako Corp.
MHCII	TAL.1B5	1:200	Dako Corp.

Organ	Region	Group	CD3	CD79α	IgG1	IgG2	IgE	MAC387	CD68	MHC II
Lymph node	Follicle	Control	1.4 ± 0.4	-	1.9 ± 1.5	4.1 ± 1.1	2.6 ± 0.5	0.3 ± 0.4	0.2 ± 0.1	23.7 ± 5.2
	Follicle	Infected	1.5 ± 0.3	-	1.2 ± 1.1	6.3 ± 2.9	1.2 ± 0.4^* * p<0.05: differences between groups in the same region analyzed and antibody; - not determined.	0.1 ± 0.2	0.2 ± 0.1	15.7 ± 4.1*
	Paracortical	Control	-	9.5 ± 1.9	0.1 ± 0.1	2.5 ± 1.0	12.0 ± 4.7	0.6 ± 0.5	0.9 ± 0.6	17.1 ± 2.7
	Paracortical	Infected	-	10.4 ± 2.9	0.3 ± 0.0	2.3 ± 0.5	2.1 ± 2.5*	0.9 ± 0.8	0.4 ± 0.3	15.6 ± 1.4
	Medulla	Control	22.2 ± 2.9	23.2 ± 4.6	11.9 ± 8.4	20.0 ± 6.2	25.8 ± 6.2	2.7 ± 3.2	9.0 ± 4.6	24.7 ± 3.6
	Medulla	Infected	30.7 ± 7.7*	38.3 ± 4.9*	4.3 ± 2.4	35.3 ± 5.1*	10.2 ± 5.6*	2.7 ± 2.8	9.1 ± 2.8	15.6 ± 4.4*
Spleen	Follicle	Control	6.3 ± 1.2	-	0.9 ± 0.8	2.1 ± 0.8	7.6 ± 2.2	0.2 ± 0.4	0.5 ± 0.3	26.8 ± 6.2
	Follicle	Infected	6.4 ± 2.2	-	0.3 ± 0.5	2.9 ± 1.4	8.0 ± 3.8	0.1 ± 0.1	0.9 ± 1.2	14.7 ± 2.0*
	Marginal zone	Control	28.8 ± 5.1	44.4 ± 1.4	1.1 ± 1.2	9.0 ± 2.0	47.3 ± 4.4	0.0 ± 0.0	7.5 ± 1.5	30.1 ± 5.1
	Marginal zone	Infected	37.2 ± 2.6*	33.1 ± 3.3*	0.8 ± 0.5	37.8 ± 10.2*	33.4 ± 5.3*	0.6 ± 0.8	9.2 ± 2.6	16.2 ± 1.8*
	Splenic cords	Control	18.6 ± 6.2	10.4 ± 1.9	0.5 ± 0.3	3.0 ± 1.5	9.6 ± 3.1	13.6 ± 4.4	9.1 ± 2.3	19.6 ± 3.0
	Splenic cords	Infected	31.6 ± 3.1	15.0 ± 3.1*	0.8 ± 0.5	13.4 ± 4.2*	3.7 ± 0.6*	18.5 ± 4.6	12.6 ± 1.3*	10.8 ± 1.3

Antigen	Organ (%)
Antigen	Liver	Kidneys	Lungs	CNS
CD3	58.4 ± 9.2	67.8 ± 3.6	39.2 ± 3.3	80.6 ± 1.8
CD79α	28.8 ± 2.6	31.8 ± 4.5	27.0 ± 2.9	10.2 ± 1.5
IgG1	1.1 ± 0.5	2.2 ± 0.8	0.7 ± 0.3	1.1 ± 1.1
IgG2	31.0 ± 3.1	32.0 ± 7.7	27.0 ± 8.2	11.0 ± 2.6
IgE	3.0 ± 1.2	0.8 ± 0.8	0.5 ± 0.3	0.0 ± 0.0
MAC387	3.6 ± 2.5	0.8 ± 0.3	1.0 ± 0.0	0.0 ± 0.0
CD68	0.7 ± 0.8	0.7 ± 0.4	0.0 ± 0.0	0.0 ± 0.0
MHCII	26.0 ± 3.8	20.2 ± 1.5	33.6 ± 4.7	-

Colégio Brasileiro de Parasitologia Veterinária FCAV/UNESP - Departamento de Patologia Veterinária, Via de acesso Prof. Paulo Donato Castellane s/n, Zona Rural, , 14884-900 Jaboticabal - SP, Brasil, Fone: (16) 3209-7100 RAMAL 7934 - Jaboticabal - SP - Brazil
E-mail: cbpv_rbpv.fcav@unesp.br

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