Anti-biofilm activity against <i>Staphylococcus aureus</i> MRSA and MSSA of neolignans and extract of <i>Piper regnellii</i>

Brambilla, Lara Z.S.; Endo, Eliana H.; Cortez, Diógenes A.G.; Dias, Benedito P.

doi:10.1016/j.bjp.2016.08.008

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Original articles • Rev. bras. farmacogn. 27 (1) • Jan-Feb 2017 • https://doi.org/10.1016/j.bjp.2016.08.008 copy

Anti-biofilm activity against Staphylococcus aureus MRSA and MSSA of neolignans and extract of Piper regnellii

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Many infections worldwide are associated with bacterial biofilms. The effects of isolated neolignans (conocarpan and eupomathenoid-5) and the dichloromethane extract of Piper regnellii (Miq.) C. DC., Piperaceae, were tested against isolates of methicillin-resistant Staphylococcus aureus and methicillin-sensitive S. aureus biofilms and S. aureus planktonic cells. The dichloromethane extract presented better results than isolated neolignans against all of the biofilms tested, with a minimum inhibitory concentration <400 µg/ml for preformed biofilms and minimal biofilm inhibitory concentration of 15.6 µg/ml for biofilm formation. The minimum inhibitory concentration to planktonic cells was <12.5 µg/ml. These results indicate a good effect of the dichloromethane extract against methicillin-resistant S. aureus and methicillin-sensitive S. aureus biofilms and efficient prophylaxis.

Keywords:
Biofilm; Conocarpan; Dichloromethane extract; Eupomathenoid-5; Staphylococcus aureus

MIC (MBC) µg/ml
Strains	Dichloromethane extract	Eupomathenoid-5	Conocarpan	Vancomycin
72	12.5 (12.5)	6.25 (25)	50 (100)	1.56 (1.56)
73	12.5 (12.5)	6.25 (25)	50 (100)	1.56 (1.56)
74	6.25 (12.5)	6.25 (6.25)	25 (25)	1.56 (1.56)
76	6.25 (25)	6.25 (12.5)	25 (25)	1.56 (1.56)
77	12.5 (25)	6.25 (12.5)	25 (25)	1.56 (1.56)
78	6.25 (12.5)	3.13 (6.25)	12.5 (100)	1.56 (1.56)
79	12.5 (25)	3.13 (6.25)	25 (25)	1.56 (1.56)
81	12.5 (25)	3.13 (6.25)	25 (50)	1.56 (1.56)
83	6.25 (12.5)	3.13 (6.25)	25 (100)	1.56 (1.56)
90	6.25 (25)	3.13 (6.25)	25 (100)	1.56 (1.56)
97	12.5 (25)	6.25 (6.25)	12.5 (12.5)	1.56 (1.56)
170	12.5 (25)	6.25 (12.5)	12.5 (12.5)	1.56 (1.56)
212	12.5 (25)	6.25 (12.5)	12.5 (25)	1.56 (1.56)
ATCC 25923	6.25 (12.5)	6.25 (12.5)	12.5 (25)	1.56 (1.56)

MIC₅₀ (µg/ml)
Strains	Dichloromethane extract	Eupomathenoid-5	Conocarpan	Vancomycin
72	300	–	>1000	–
73	170	–	–	–
74	50	–	–	–
76	360	–	–	–
77	390	–	>1000	–
78	180	–	>1000	–
79	190	–	>1000	–
81	210	–	>1000	–
83	230	–	>1000	–
90	130	–	>1000	–
97	260	–	>1000	–
170	260	–	>1000	–
212	370	–	>1000	–
ATCC 25923	370	–	110	–

MBIC (µg/ml)
Strains	Dichloromethane extract	Eupomathenoid-5	Conocarpan	Vancomycin
72	15.6	15.6	15.6	7.8
73	15.6	15.6	15.6	7.8
74	15.6	31.2	15.6	7.8
76	15.6	31.2	15.6	7.8
77	15.6	250	125	7.8
78	15.6	15.6	15.6	7.8
79	15.6	15.6	15.6	15.6
81	15.6	15.6	15.6	7.8
83	31.2	31.2	31.2	62.5
90	15.6	15.6	15.6	7.8
97	15.6	15.6	15.6	7.8
170	15.6	15.6	15.6	7.8
212	15.6	15.6	15.6	7.8
ATCC 25923	15.6	31.2	15.6	7.8

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[1] * Corresponding author. E-mail:bpdfilho@uem.br (B.P. Dias Filho).