Marine organisms as source of extracts to disrupt bacterial communication: bioguided isolation and identification of quorum sensing inhibitors from <i>Ircinia felix</i>

Quintana, Jairo; Brango-Vanegas, José; Costa, Geison M.; Castellanos, Leonardo; Arévalo, Catalina; Duque, Carmenza

doi:10.1016/j.bjp.2015.03.013

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Revista Brasileira de Farmacognosia

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Original Articles • Rev. bras. farmacogn. 25 (3) • May-Jun 2015 • https://doi.org/10.1016/j.bjp.2015.03.013 copy

Marine organisms as source of extracts to disrupt bacterial communication: bioguided isolation and identification of quorum sensing inhibitors from Ircinia felix

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Abstract

In this study, 39 extracts from marine organisms were evaluated as quorum sensing inhibitors, collected in the Colombian Caribbean Sea and the Brazilian Coast including 26 sponges, seven soft corals, five algae and one zooanthid. The results showed that crude extracts from the soft coral Eunicea laciniata, and the sponges Svenzea tubulosa, Ircinia felix and Neopetrosia carbonaria were the most promising source of quorum sensing inhibitors compounds without affecting bacterial growth, unlike the raw extracts of Agelas citrina, Agelas tubulata, Iotrochota arenosa, Topsentia ophiraphidites, Niphates caycedoi, Cliona tenuis, Ptilocaulis walpersi, Petrosia pellasarca, and the algae Laurencia catarinensis and Laurencia obtusa, which displayed potent antibacterial activity against the biosensors employed. The crude extract from the sponge I. felix was fractionated, obtaining furanosesterterpenes which were identified and evaluated as quorum sensing inhibitors, showing a moderate activity without affecting the biosensor's growth.

Keywords
Quorum sensing; Marine products; Ircinia felix ; Furanosesterterpenes; Biofilm

Type of collected sample	Marine organism extract source	Collection number	Collection date	Geographic origin	Escherichia coli pSB401				Chromobacterium violaceum ATCC 31532
					Growing inhibition^a a halo, in mm.		QS inhibition^a a halo, in mm.		Growing inhibition^a a halo, in mm.			QS inhibition^a a halo, in mm.
					200 μg	400 μg	200 μg	400 μg	100 μg	200 μg	400 μg	100 μg	200 μg	400 μg
Sponges	Agelas citrina	INV-POR 969	05/04/2008	Islas del Rosario (Col)	7–9	7–9	5–7	>12	-	-	-	-	-	7–9
	Agelas tubulata	ICN-MHN(Po) 154	07/12/2005	Santa Marta (Col)	7–9	7–9	-	>12	-	–	NT	–	–	NT
	Agelas marron	ICN-MHN(Po) 0149	07/12/2005	Islas del Rosario (Col)	–	–	–	–	–	–	–	–	–	–
	Aka cachacrouense	INV-POR-412	07/12/2005	Santa Marta (Col)	–	5–7	–	–	–	–	NT	9–12	9–12	NT
	Biemna cribaria	INV-POR-890	07/12/2005	Santa Marta (Col)	7–9	5–7	–	7–9	NT	–	–	NT	–	–
	Cinachyrella kuekenthalli	INV-POR-878	18/11/2009	Santa Marta (Col)	–	–	5–7	5–7	NT	NT	NT	NT	NT	NT
	Cliona delitrix	INV-MHN(Po)-189	18/09/2005	San Andrés (Col)	–	–	–	7–9	NT	–	–	NT	–	–
	Cliona tenuis	INV-POR-669	06/04/2008	Islas del Rosario (Col)	–	7–9	–	–	9–12	9–12	NT	–	–	NT
	Cliona varians	INV-POR-339	07/09/2005	Santa Marta (Col)	–	–	–	–	NT	–	–	NT	–	–
	Iotrochota arenosa (Iotrochota inminuta)^b b Formerly known as: –, no activity halo, <5 mm; NT , no tested; DCM, dichloromethane fraction; WB, butanol fraction; WW, aqueous fraction; Col, Colombia; Bra, Brasil.	INV-POR-883	15/11/2006	Santa Marta (Col)	>12	–	–	7–9	–	–	–	–	–	–
	Ircinia felix	INV-POR-014	18/11/2009	Santa Marta (Col)	NT	NT	NT	NT	–	–	–	7–9	7–9	7–9
	Lissodendoryx colombiensis	ICN-MHN-PO 0246	06/04/2008	Islas del Rosario (Col)	–	–	–	–	NT	–	–	NT	–	–
	Muriceopsis sp.	ICN-MHN-PO 0254	13/02/2006	Santa Marta (Col)	–	5–7	–	–	–	–	NT	-	–	NT
	Neopetrosia carbonaria (DCM) (Xestospongia carbonaria)^b b Formerly known as: –, no activity halo, <5 mm; NT , no tested; DCM, dichloromethane fraction; WB, butanol fraction; WW, aqueous fraction; Col, Colombia; Bra, Brasil.	ICN-MHN-PO 0244	05/04/2008	Islas del Rosario (Col)	–	–	–	7–9	–	–	–	>12	–	9–12
	Neopetrosia carbonaria (WB)	ICN-MHN-PO 0244	05/04/2008	Islas del Rosario (Col)	–	–	7–9	7–9	–	–	–	–	–	>12
	Neopetrosia carbonaria (WW)	ICN-MHN-PO 0244	05/04/2008	Islas del Rosario (Col)	–	–	–	–	NT	–	–	NT	–	–
	Neopetrosia muta (Xestospongia muta)^b b Formerly known as: –, no activity halo, <5 mm; NT , no tested; DCM, dichloromethane fraction; WB, butanol fraction; WW, aqueous fraction; Col, Colombia; Bra, Brasil.	ICN-MHN-PO 0247	06/04/2008	Islas del Rosario (Col)	–	–	–	–	NT	–	–	NT	–	–
	Neopetrosia proxima (Xestospongia proxima)^b b Formerly known as: –, no activity halo, <5 mm; NT , no tested; DCM, dichloromethane fraction; WB, butanol fraction; WW, aqueous fraction; Col, Colombia; Bra, Brasil.	ICN-MHN-PO 0239	04/04/2008	Islas del Rosario (Col)	–	–	–	–	NT	–	–	NT	–	–
	Neopetrosia rosariensis (Xestospongia rosariensis)^b b Formerly known as: –, no activity halo, <5 mm; NT , no tested; DCM, dichloromethane fraction; WB, butanol fraction; WW, aqueous fraction; Col, Colombia; Bra, Brasil.	ICN-MHN-PO 0245	05/04/2008	Islas del Rosario (Col)	–	–	–	-	NT	–	–	NT	–	–
	Neopetrosia subtriangularis (Xestospongia subtriangularis)^b b Formerly known as: –, no activity halo, <5 mm; NT , no tested; DCM, dichloromethane fraction; WB, butanol fraction; WW, aqueous fraction; Col, Colombia; Bra, Brasil.	ICN-MHN-PO 0242	05/04/2008	Islas del Rosario (Col)	–	–	–	–	–	–	–	>12	7–9	7–9
	Ptilocaulis walpersi	ICN-MHN-PO 0248	06/04/2008	Islas del Rosario (Col)	–	–	–	5–7	NT	9–12	9–12	NT	–	–
	Svenzea tubulosa (crude extract)	ICN-MHN-PO 0249	06/04/2008	Islas del Rosario (Col)	–	–	9–12	>12	–	–	–	–	>12	9–12
	Svenzea tubulosa (DCM)	ICN-MHN-PO 0249	06/04/2008	Islas del Rosario (Col)	NT	NT	NT	NT	–	–	–	7–9	9–12	9–12
	Topsentia ophiraphidites	ICN-MHN-PO 0243	05/04/2008	Islas del Rosario (Col)	–	9–12	–	5–7	–	–	–	–	–	–

Soft corals	Eunicea laciniata	ICN-MHN-CR-106	13/02/2006	Santa Marta (Col)	–	–	7–9	7–9	NT	NT	NT	NT	NT	NT
	Eunicea succinea	ICN-MHN-PO 0251	13/02/2006	Santa Marta (Col)	–	–	–	5–7	NT	–	–	NT	–	–
	Eunicea fusca	ICN-MHN-PO 0252	13/02/2006	Santa Marta (Col)	–	–	–	5–7	NT	–	–	NT	–	–
	Eunicea sp1	–	13/02/2006	Santa Marta (Col)	5–7	5–7	–	–	NT	–	–	NT	–	–
	Erythropodium caribaeorum	INV-CNI-1193	07/09/2005	Santa Marta (Col)	–	–	–	–	–	–	–	–	–	9–12
	Pseudopterogorgia elisabethae (Providencia)	INV-CNI-1612–1614	25/05/2008	Providencia and San Andrés (Col)	5–7	–	–	–	–	–	NT	–	–	NT
	Pseudopterogorgia elisabethae (San Andrés)	INV-CNI-1615-1616	18/09/2005	Providencia and San Andrés (Col)	–	–	–	9–12	NT	–	–	NT	–	–

Zoanthid	Palythoa caribaeorum	ICN-MHN-PO 0255	07/09/2005	Santa Marta (Col)	–	–	–	–	–	–	–	–	9–12	–

Algae	Laurencia catarinensis	FLOR 14516	02/2008	Ilha do Arvoredo, Florianópolis (Bra)	–	–	–	–	7–9	9–12	9–12	–	–	–
	Laurencia flagellifera	FLOR 14521	03/2006	Praia da Sepultura, Bombinhas (Bra)	–	–	–	–	–	–	–	–	–	–
	Laurencia majuscule	FLOR 14524	11/2006	Ilha do Francês, Florianópolis (Bra)	–	–	–	–	–	7–9	–	–	–	–
	Laurencia microcladia	FLOR 14520	03/2008	Praia da Sepultura, Bombinhas (Bra)	–	–	–	–	–	–	–	–	–	–
	Laurencia obtusa	FLOR 14512	08/2009	Praia de Canasvieiras, Florianópolis (Bra)	–	–	–	–	7–9	9–12	>12	–	–	–

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[1] * Corresponding author. E-mail: lcastellanosh@unal.edu.co (L. Castellanos).